Virosomes Constructed from Lipid and Purified Friend Leukaemia Virus Glycoprotein Free

Abstract

SUMMARY

Liposomes were loaded by a dialysis technique with purified envelope glycopolypeptide, gp85, of the Friend murine leukaemia virus (F-MuLV). The gp85 liposomes prepared from cellular lipid had a buoyant density of 1.05 g/ml and an apparent diameter of 50 to 300 nm. The gp85 was not simply entrapped by liposomes nor adsorbed non-specifically to their outer surface. Experiments with radioactively labelled protein, electron microscopic examinations, protease treatment and concanavalin A binding showed that gp85 is anchored in the liposomal membrane and orientated asymmetrically as in the virus envelope. Moreover, gp85-covered liposomes displayed some functions of the intact F-MuLV envelope, such as absorption of antibodies to gp70 and haemagglutination after enzyme treatment. To some extent, these lipid vesicles appeared to be reconstituted F-MuLV envelopes and thus, by analogy to other systems, were named retrovirosomes.

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1983-03-01
2024-03-28
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References

  1. Almeida J. D., Edwards D. C., Brand C. M., Heath T. D. 1975; Formation of virosomes from influenza subunits and liposomes. Lancet ii:899–901
    [Google Scholar]
  2. Bercovici T., Gitler C. 1978; 5-[12SI]iodonaphthylazide, a reagent to determine the penetration of proteins into the lipid bilayer of biological membranes. Biochemistry 17:1484–1489
    [Google Scholar]
  3. Bligh E. G., Dyer W. J. 1959; A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry 37:911–916
    [Google Scholar]
  4. Bolton A. E., Hunter W. M. 1973; The labelling of proteins to high specific activities by conjugation to a 125I-containing acylating agent. Biochemical Journal 133:529–539
    [Google Scholar]
  5. Greenwood F. C., Hunter W. M., Glover J. S. 1963; The preparation of iodine 131-labelled human growth hormone of high specific activity. Biochemical Journal 89:114–123
    [Google Scholar]
  6. Hartree E. F. 1972; A modification of the Lowry method that gives a linear photometric response. Analytical Biochemistry 48:422–426
    [Google Scholar]
  7. Helenius A., Fries E., Kartenbeck J. 1977; Reconstitution of Semliki Forest virus membrane. Journal of Cell Biology 75:866–880
    [Google Scholar]
  8. Huang R. T. C., Wahn K., Klenk H.-D., Rott R. 1979; Association of the envelope glycoproteins of influenza virus with liposomes – a model study on viral envelope assembly. Virology 97:212–217
    [Google Scholar]
  9. Huang R. T. C., Wahn K., Klenk H.-D., Rott R. 1980; Fusion between cell membrane and liposomes containing the glycoproteins of influenza virus. Virology 104:294–302
    [Google Scholar]
  10. Hunsmann G., Schneider J., Schulz A. 1981; Immunoprevention of Friend virus erythroleukemia by vaccination with viral envelope glycoprotein complexes. Virology 113:602–612
    [Google Scholar]
  11. Hunsmann G., Pedersen N. C., Theilen G. H., Bayer H. 1982; Active immunization with feline leukemia virus envelope glycoprotein suppresses growth of virus-induced feline sarcoma. Medical Microbiology and Immunology (in press)
    [Google Scholar]
  12. Koch W., Hunsmann G., Friedrich R. 1983; Nucleotide sequence of the envelope gene of Friend murine leukemia virus. Journal of Virology (in press)
    [Google Scholar]
  13. Koszinowski U. H., Allen H., Gething M.-J., Waterfield M.-D., Klenk H. D. 1980; Recognition of viral glycoproteins by influenza A-specific cross reactive cytolytic T lymphocytes. Journal of Experimental Medicine 151:945–958
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 221:680–685
    [Google Scholar]
  15. Loh D., Ross A. H., Hale A. H., Baltimore D., Eisen H. N. 1979; Synthetic phospholipid vesicles containing a purified viral antigen and cell membrane proteins stimulate the development of cytotoxic T lymphocytes. Journal of Experimental Medicine 150:1067–1074
    [Google Scholar]
  16. McGrath M. S., Weissmann I. L. 1979; AKR leukemogenesis: identification and biological significance of thymic lymphoma receptors for AKR retroviruses. Cell 17:65–75
    [Google Scholar]
  17. Moennig V., Frank H., Hunsmann G., Schneider I., Schafer W. 1974; Properties of mouse leukemia viruses. VII. The major viral glycoprotein of Friend leukemia virus. Isolation and physicochemical properties. Virology 61:100–111
    [Google Scholar]
  18. Morein B., Helenius A., Simons K., Pettersson R., Kääriäinen L., Schirrmacher V. 1978; Effective subunit vaccines against an enveloped animal vims. Nature, London 276:715–718
    [Google Scholar]
  19. Nermut M. V., Frank H., Schafer W. 1972; Properties of mouse leukemia viruses. 111. Electron microscopic appearance as revealed after conventional techniques as well as freeze-drying and freeze-etching. Virology 49:345–358
    [Google Scholar]
  20. Schneider J., Schwarz H., Hunsmann G. 1979; Rosettes from Friend leukemia virus envelope: preparation and physicochemical and partial biological characterization. Journal of Virology 29:624–632
    [Google Scholar]
  21. Schneider J., Falk H., Hunsmann G. 1980; Envelope polypeptides of Friend leukemia vims: purification and structural analysis. Journal of Virology 33:597–605
    [Google Scholar]
  22. Schneider J., Falk H., Hunsmann G. 1981; Purification of murine and feline type-C virus envelope polypeptides as micellar protein complexes. Zeitschrift für Naturforschung 36c:353–356
    [Google Scholar]
  23. Witter R., Frank H., Moenning V., Hunsmann G., Lange J., Schafer W. 1973; Properties of mouse leukemia viruses. IV. Hemagglutination assay and characterization of hemagglutinating surface components. Virology 54:330–345
    [Google Scholar]
  24. Zarling D. A., Keshet I. 1979; Fusion activity of murine leukemia vims. Virology 95:185–196
    [Google Scholar]
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