@article{mbs:/content/journal/jgv/10.1099/0022-1317-64-3-539, author = "Zerbini, M. and Ernberg, I.", title = "Can Epstein–Barr Virus Infect and Transform All the B-Lymphocytes of Human Cord Blood?", journal= "Journal of General Virology", year = "1983", volume = "64", number = "3", pages = "539-547", doi = "https://doi.org/10.1099/0022-1317-64-3-539", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-3-539", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "lymphocytes", keywords = "EBV", keywords = "EBNA", abstract = "SUMMARY Quantitative aspects of Epstein—Barr virus infection and transformation of human neonatal B-lymphocytes have been investigated. 72 to 90% B-cells were obtained with enrichment. Of the B-cells, 19 to 97% showed nuclear antigen (EBNA) 2 days after infection. A difference between different B-cell donors in susceptibility to infection was noted. Analysis of the virus dose—response curves obtained with twofold virus dilutions showed that one virus particle is sufficient to induce EBNA in a cell. Of the infected cells, 50 to 95% multiplied in microtitre wells containing a human fibroblast feeder layer, while only a small proportion established growing colonies in soft agarose, that could be picked up and subcultured.", }