RT Journal Article SR Electronic(1) A1 Herrero-Uribe, L. A1 Mann, G. F. A1 Zuckerman, A. J. A1 Hockley, D. A1 Oxford, J. S.YR 1983 T1 Replication of Influenza A and B Viruses in Human Diploid Cells JF Journal of General Virology, VO 64 IS 2 SP 471 OP 475 DO https://doi.org/10.1099/0022-1317-64-2-471 PB Microbiology Society, SN 1465-2099, AB SUMMARY Under optimal conditions, of high multiplicities of infection and with trypsin included in the medium throughout the incubation period, high yields of infectious influenza A and B viruses (106.5 p.f.u./ml) and of antigenically active haemagglutinin (HA) (1 µg/HA/106 cells) were produced in human diploid MRC-5 cells. Budding virus particles were seen as spherical or short rod-like protrusions on the surface of the infected cells, and also on cell filopodia. Virus-induced cytoplasmic and nuclear inclusions were present in infected cells. This virus-human cell system may be suitable for studies of influenza virus persistence and for production of immunologically active HA antigen., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-2-471