%0 Journal Article %A Herrero-Uribe, L. %A Mann, G. F. %A Zuckerman, A. J. %A Hockley, D. %A Oxford, J. S. %T Replication of Influenza A and B Viruses in Human Diploid Cells %D 1983 %J Journal of General Virology, %V 64 %N 2 %P 471-475 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-64-2-471 %K antigens %K influenza virus %K diploid cells %K replication %I Microbiology Society, %X SUMMARY Under optimal conditions, of high multiplicities of infection and with trypsin included in the medium throughout the incubation period, high yields of infectious influenza A and B viruses (106.5 p.f.u./ml) and of antigenically active haemagglutinin (HA) (1 µg/HA/106 cells) were produced in human diploid MRC-5 cells. Budding virus particles were seen as spherical or short rod-like protrusions on the surface of the infected cells, and also on cell filopodia. Virus-induced cytoplasmic and nuclear inclusions were present in infected cells. This virus-human cell system may be suitable for studies of influenza virus persistence and for production of immunologically active HA antigen. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-2-471