1887

Abstract

SUMMARY

Under optimal conditions, of high multiplicities of infection and with trypsin included in the medium throughout the incubation period, high yields of infectious influenza A and B viruses (10 p.f.u./ml) and of antigenically active haemagglutinin (HA) (1 µg/HA/10 cells) were produced in human diploid MRC-5 cells. Budding virus particles were seen as spherical or short rod-like protrusions on the surface of the infected cells, and also on cell filopodia. Virus-induced cytoplasmic and nuclear inclusions were present in infected cells. This virus-human cell system may be suitable for studies of influenza virus persistence and for production of immunologically active HA antigen.

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1983-02-01
2022-01-17
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