1887

Abstract

SUMMARY

Polyvalent serum directed against C3 receptors was employed in an attempt to block Epstein—Barr virus (EBV) binding to virus receptor-containing cell lines. The serum eliminated 90% of virus binding to Daudi and BJAB, lines which express only the C3d receptor. Raji and Ramos cells, which express the C3d, C3b and C3bi receptors, still adsorbed 70% of their virus capacity in the presence of excess antiserum. These effects were independent of the virus strain. In the light of previous reports, these data imply that, although the two receptors, EBV and C3d, are closely associated, the binding sites of EBV and complement are distinct. Additionally, an unusual EBV substrain-specific receptor found on U698 and P3HR-1/ASNP lines was shown to be independent of complement receptors.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-2-449
1983-02-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/2/JV0640020449.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-2-449&mimeType=html&fmt=ahah

References

  1. Abu Sinna G., Lundgren E., Nilsson K., Roos G. 1980; Isozymes of amino acid naphthylamidase in human haemapoietic cell lines. British Journal of Haematology 44:25–32
    [Google Scholar]
  2. Epstein M. A., Achong B. (editors) 1979; The Epstein-Barr virus. Berlin, Heidelberg & New York: Springer-Verlag;
    [Google Scholar]
  3. Epstein M. A., Achong B., Barr Y., Zajac B., Henle G., Henle W. 1966; Morphological and virological investigation of cultured Burkitt tumor lymphoblasts (strain Raji). Journal of the National Cancer Institute 37:547–559
    [Google Scholar]
  4. Fresen K., Zur Hausen H. 1976; Establishment of EBNA-expressing cell lines by infection of EBV-genome negative human lymphoma cells with different EBV strains. International Journal of Cancer 17:161–166
    [Google Scholar]
  5. Gerdes J., Stein H. 1981; Human membrane-bound C3 receptors. II. Physicochemical properties of the C3b and C3d receptors isolated from tonsil cells by immunoprecipitation. Scandinavian Journal of Immunology 13:77–86
    [Google Scholar]
  6. Gerdes J., Stein H., Dierich M. P., Conrad D. H. 1982; Antisera with anti-pan C3 receptor reactivity which block complement C3b, C3bi and C3d receptors. Journal of Immunological Methods 51:259–265
    [Google Scholar]
  7. Jondal M., Klein G., Oldstone M., Bokish V., Yefenof E. 1976; Surface markers on human B and T lymphocytes. VIII. Association between complement and Epstein-Barr virus receptors on human lymphoid cells. Scandinavian Journal of Immunology 5:401–410
    [Google Scholar]
  8. Jønsson V., Wells A., Klein G. 1982; Receptors for the complement C3d component and the Epstein-Barr virus are quantitatively co-expressed on a series of B-cell lines and their derived somatic cell hybrids. Cellular Immunology 72: (in press)
    [Google Scholar]
  9. Klein G., Yefenof E., Falk K., Westman A. 1978; Relationship between Epstein-Barr virus (EBV)-production and the loss of the EBV receptor/complement receptor complex in a series of sublines derived from the same original Burkitt’s lymphoma. International Journal of Cancer 21:552–560
    [Google Scholar]
  10. Klein G., Manneborg A., Steinitz M. 1979; Differences in EBV receptor concentration between in vitro EBV-converted lymphoma sublines reflect biological differences between the converting viral substrains. International Journal of Cancer 23:197–200
    [Google Scholar]
  11. Koide N., Wells A., Volsky D., Shapiro I., Klein G. 1981; The detection of Epstein-Barr virus receptors utilizing radiolabelled virus. Journal of General Virology 54:191–195
    [Google Scholar]
  12. Lambris D. J., Dobson N. J., Ross G. D. 1981; Isolation of lymphocyte membrane complement receptor type two (the C3d receptor) and preparation of receptor-specific antibody. Proceedings of the National Academy of Sciences of the United States of America 78:1828–1832
    [Google Scholar]
  13. Nilsson K., Sundstrom C. 1974; Establishment and characteristics of two unique cell lines from patients with lymphosarcoma. International Journal of Cancer 13:808–823
    [Google Scholar]
  14. Stein H., Gerdes J., Tolksdorf G., Klatt U. 1981; Human membrane-bound C3 receptors. I. Serological and immunohistological demonstration of C3 receptors. Scandinavian Journal of Immunology 13:67–76
    [Google Scholar]
  15. Wells A., Koide N., Klein G. 1981; Difference in viral binding between two Epstein-Barr virus substrains to a spectrum of receptor-positive target cells. International Journal of Cancer 27:303–309
    [Google Scholar]
  16. Yefenof E., Klein G. 1977; Membrane receptor stripping confirms the association between EBV receptors and complement receptors on the surface of human B lymphoma lines. International Journal of Cancer 20:347–352
    [Google Scholar]
  17. Yefenof E., Klein G., Jondal M., Oldstone M. 1976; Surface markers on human B and T lymphocytes. IX. Two color immunofluorescence studies on the association between EBV receptors and complement receptors on the surface of lymphoid cells lines. International Journal of Cancer 17:693–700
    [Google Scholar]
  18. Yefenof E., Klein G., Kvarnung K. 1977; Relationship between complement activation, complement binding, and EBV absorption by human hemapoietic cell lines. Cellular Immunology 31:225–233
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-2-449
Loading
/content/journal/jgv/10.1099/0022-1317-64-2-449
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error