1887

Abstract

SUMMARY

The extent of methylation of several avian oncogenic proviruses was determined by using the restriction endonucleases II and I. The results indicated that the transformation-defective proviruses (RAV-O or B77-td), which are exogenously introduced into avian host cells, were not methylated. However, endogenous proviruses (RAV-O) or ASV proviruses present in non-permissive host cells were found to be partly or completely methylated. The methyl-sensitive restriction endonuclease I, which recognizes a unique site within the long terminal repeat in the ASV genome, failed to cleave proviruses present in several non-permissive host cells. From these results we suggest that modification of the sequence around the I site results in reduced levels of transcription.

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1983-02-01
2022-01-21
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References

  1. Bird A. P., Southern E. M. 1978; Use of restriction enzymes to study eukaryotic DNA methylation. I. The methylation pattern in ribosomal DNA from Xenopus laevis. Journal of Molecular Biology 118:27–47
    [Google Scholar]
  2. Cedar H., Solge A., Glaser G., Razin A. 1979; Direct detection of methylated cytosine in DNA by the use of the restriction enzyme Msp I. Nucleic Acids Research 6:2125–2132
    [Google Scholar]
  3. Copeland N. G., Jenkins N. A., Cooper G. M. 1981; Integration of Rous sarcoma virus during transfection. Cell 23:51–60
    [Google Scholar]
  4. De Lorbe W. J., Luciw P. A., Goodman H. M., Varmus H. E., Bishop J. M. 1980; Molecular cloning and characterization of avian sarcoma virus circular DNA molecules. Journal of Virology 36:50–61
    [Google Scholar]
  5. Doerfler W. 1981; DNA methylation—A regulatory signal in eukaryotic gene expression. Journal of General Virology 57:1–20
    [Google Scholar]
  6. Doscocil J., Sorm F. 1962; Distribution of 5-methycytosine pyrimidine sequences of deoxyribonucleic acids. Biochimica et biophysica acta 55:953–962
    [Google Scholar]
  7. Groudine M., Eisenman R., Weintraub H. 1981; Chromatin structure of endogenous retroviral genes and activation by an inhibitor of DNA methylation. Nature, London 292:311–317
    [Google Scholar]
  8. Guntaka R. V., Rao P., Mitsialis S. A., Katz R. A. 1980; Modification of avian sarcoma proviral DNA sequences in non-permissive XC cells, but not in permissive chicken cells. Journal of Virology 34:569–572
    [Google Scholar]
  9. Harbers K., Schnieke A., Stuhlman H., Jahner D., Jaenisch R. 1982; DNA methylation and gene expression: endogenous retroviral genome becomes infectious after molecular cloning. Proceedings of the National Academy of Sciences of the United States of America 78:7609–7613
    [Google Scholar]
  10. Hsu T. W., Sabaran J. L., Mark G. E., Guntaka R. V., Taylor J. M. 1978; Analysis of unintegrated avian RNA tumor virus double-stranded DNA intermediates. Journal of Virology 28:810–818
    [Google Scholar]
  11. Humphries E. A., Glover C., Weiss R. A., Armand J. R. 1979; Differences between endogenous and exogenous DNA sequences of Rous-associated virus-O. Cell 18:803–815
    [Google Scholar]
  12. Jones P. A., Taylor S. M. 1980; Cellular differentiation, cytidine analogs, and DNA methylation. Cell 20:85–93
    [Google Scholar]
  13. Katz R. A., Omer C., Weiss J. H., Mitsialis S. A., Faras A. J., Guntaka R. V. 1982; Restriction endonuclease and nucleotide sequence analyses of molecularly cloned unintegrated avian tumor virus DNA: Structure of large terminal repeats in circle junctions. Journal of Virology 42:346–351
    [Google Scholar]
  14. McClements W., Hanafusa H., Tilghman S., Skalka A. 1979; Structural studies on oncornavirus-related sequences in chicken genomic DNA. Proceedings of the National Academy of Sciences of the United States of America 76:2165–2169
    [Google Scholar]
  15. Nichols W. W. 1963; Relationships of viruses, chromosomes and carcinogenesis. Hereditas 50:53–80
    [Google Scholar]
  16. Razin A., Riggs A. D. 1980; DNA methylation and gene function. Science 210:604–610
    [Google Scholar]
  17. Shank P. R., Hughes S. H., Rung H. J., Majors J. E., Quintrell N., Guntaka R. V., Bishop J. M., Varmus H. E. 1978; Mapping integrated avian sarcoma virus DNA. Termini of linear DNA bear 300 nucleotides present once or twice in two species of circular DNA. Cell 15:1383–1395
    [Google Scholar]
  18. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  19. Tantravahi U., Guntaka R. V., Erlanger B. F., Miller O. J. 1981; Amplified ribosomal RNA genes in a rat hepatoma cell line are enriched in 5-methylcytosine. Proceedings of the National Academy of Sciences of the United States of America 78:489–493
    [Google Scholar]
  20. Temin H. M. 1982; Function of the retrovirus long terminal repeat. Cell 28:3–5
    [Google Scholar]
  21. Vanyushin B. F., Tracheva S. G., Berlozersky A. N. 1970; Rare bases in animal DNA. Nature, London 225:948–949
    [Google Scholar]
  22. Yamamoto T., De Cromburgghe B., Pastan I. 1980; Identification of a functional promoter in the long terminal repeat of Rous sarcoma virus. Cell 22:787–797
    [Google Scholar]
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