1887

Abstract

SUMMARY

The major nucleocapsid protein of single nucleocapsid nuclear polyhedrosis virus is a low mol. wt., basic, DNA-binding protein present in the core of the capsid. It is rich in arginine and helix-destabilizing residues and possesses no lysine nor hydrophobic residues. Circular dichroism analysis showed that the protein undergoes a major conformational change in high salt solutions involving the tyrosine side chains. There is sufficient of this protein in the nucleocapsid for all the genome phosphate to be neutralized by arginine residues. A comparison of the protein with similar basic proteins from granulosis virus, non-occluded baculovirus, and multiple nucleocapsid nuclear polyhedrosis virus showed that they are all arginine-rich, lysine-poor, DNA-binding proteins.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-2-399
1983-02-01
2024-04-19
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/2/JV0640020399.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-2-399&mimeType=html&fmt=ahah

References

  1. Brahms S., Brahms I. 1980; Determination of protein secondary structure in solution by vacuum ultraviolet circular dichroism. Journal of Molecular Biology 138:149–178
    [Google Scholar]
  2. Brown D. A., Bud H. M., Kelly D. C. 1977; Biophysical and biochemical properties of the structural components of a granulosis virus isolated from the cabbage white butterfly (Pieris brassicae). Virology 81:317–327
    [Google Scholar]
  3. Brown D. A., Evans H. F., Allen C. J., Kelly D. C. 1981; Biological and biochemical investigations on five European isolates of Mamestra brassicae nuclear polyhedrosis virus. Archives of Virology 69:209–217
    [Google Scholar]
  4. Bud H. M., Kelly D. C. 1980; An electron microscope study of partially lysed baculovirus nucleocapsids: the intranucleocapsid packaging of viral DNA. Journal of Ultrastructure Research 73:361–380
    [Google Scholar]
  5. Burley S. K., Miller A., Harrap K. A., Kelly D. C. 1982; Structure of the baculovirus nucleocapsid. Virology 120:433–440
    [Google Scholar]
  6. Elliott R. M., Kelly D. C. 1977; The polyamine content of a nuclear polyhedrosis virus from Spodoptera littoralis. Virology 76:472–474
    [Google Scholar]
  7. Elliott R. M., Kelly D. C. 1979; Compartmentalization of the polyamines contained by a nuclear polyhedrosis virus from Heliothis zea. Microbiologica 2:409–413
    [Google Scholar]
  8. Kelly D. C., Wang X. 1981; The infectivity of nuclear polyhedrosis virus DNA. Annals de Virologie 132E:247–259
    [Google Scholar]
  9. Kelly D. C., Barwise A. H., Walker I. O. 1977; DNA contained by two densonucleosis viruses. Journal of Virology 21:396–407
    [Google Scholar]
  10. Kelly D. C., Ayres M. D., Lescott T., Robertson J. S., Happ G. M. 1979; A small iridescent virus (type 29) isolated from Tenebrio molitor: a comparison of its proteins and antigens with six other iridescent viruses. Journal of General Virology 42:95–105
    [Google Scholar]
  11. Kelly D. C., Brown D. A., Robertson J. S., Harrap K. A. 1980; Biochemical, biophysical and serological properties of two singly enveloped nuclear polyhedrosis viruses from Heliothis armigera and Heliothis zea. Microbiologica 3:319–331
    [Google Scholar]
  12. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  13. Leonard K. R. 1970; Physical studies on subunit interactions in oligomeric enzymes. D.Phil thesis University of Oxford;
    [Google Scholar]
  14. Mcpherson A., Jurnak F., Wang A., Molineux I., Rich A. 1979; Preliminary molecular replacement results for a crystalline gene 5 protein deoxynucleotide complex. Journal of Supramolecular Structure 10:457–465
    [Google Scholar]
  15. Maniatis T., Jeffrey A., Kleid D. C. 1975; Nucleotide sequence of the righthand operator of phage λ. Proceedings of the National Academy of Sciences of the United States of America 72:1184–1188
    [Google Scholar]
  16. Panyim S., Chalkley R. 1969; High resolution polyacrylamide gel electrophoresis of histones. Archives of Biochemistry and Biophysics 130:337–346
    [Google Scholar]
  17. Payne C. C., Compson D., De Looze S. M. 1977; Properties of the nucleocapsids of a virus isolated from Oryctes rhinoceros. Virology 77:269–280
    [Google Scholar]
  18. Peacocke A. R., Walker I. O. 1962; The thermal denaturation of sodium deoxyribonucleate. Journal of Molecular Biology 5:550–559
    [Google Scholar]
  19. Revet B. M. J., Guelpa B. 1979; The genome of a baculovirus infecting Tipulapaludosa (Meig.) (Diptera): a high molecular weight closed circular DNA of zero superhelical density. Virology 96:633–639
    [Google Scholar]
  20. Rigby P. W. J., Dieckman N. M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  21. Shapiro A. L., Vinuela E., Maizel J. V. 1967; Molecular weight estimation of polypeptide chains by electrophoresis in SDS polyacrylamide gels. Biochemical and Biophysical Research Communications 28:815–820
    [Google Scholar]
  22. Summers M. D., Smith G. E. 1978; Baculovirus structural proteins. Virology 84:390–402
    [Google Scholar]
  23. Tweeten K. A., Bulla L. A., Consigli R. A. 1980; Characteristics of an extremely basic protein derived from granulosis virus nucleocapsids. Journal of Virology 33:866–876
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-2-399
Loading
/content/journal/jgv/10.1099/0022-1317-64-2-399
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error