@article{mbs:/content/journal/jgv/10.1099/0022-1317-64-2-291, author = "Ghendon, Y. Z. and Markushin, S. G. and Klimov, A. I. and Lotte, V. D. and Ginzburg, V. P.", title = "Studies on a Temperature-sensitive Mutant of Fowl Plague Virus Having a Mutation in Gene 7 Coding for the M Protein", journal= "Journal of General Virology", year = "1983", volume = "64", number = "2", pages = "291-304", doi = "https://doi.org/10.1099/0022-1317-64-2-291", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-2-291", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "fowl plague virus", keywords = "ts mutants", keywords = "orthomyxoviruses", keywords = "M protein", abstract = "SUMMARY A fowl plague virus (FPV) temperature-sensitive mutant, ts 303/1 having a ts mutation in gene 7 coding for the matrix (M) protein has been obtained. The mutant induced synthesis of virus-specific RNA and polypeptides as well as ribonuclear protein (RNP) formation in cells under non-permissive conditions; however, haemagglutinin cleavage was reduced, functionally active haemagglutinin and neuraminidase were absent and virions were not formed. In mutant-infected cells at 36 °C haemagglutinin cleavage was also reduced and virions formed had an altered NP:M ratio as well as a decreased haemagglutinin content. A population of virions formed under these conditions was heterogeneous both in morphology and in buoyant density. The data obtained suggest that a mutation in the M proteins of orthomyxoviruses can affect processing of the haemagglutinin and impair final stages of virion morphogenesis.", }