1887

Abstract

SUMMARY

Mouse interferon (IFN) was produced to high titres after induction of Ehrlich ascites tumour cells with Sendai virus by using an improved procedure. The IFN molecules were labelled during their synthesis by the incorporation of [H]leucine and [H]lysine. Electrophoretically homogeneous labelled IFN with a molecular weight of 34000 was obtained after a two-step purification procedure using poly(I)-agarose and octyl-Sepharose column chromatography followed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The specific radioactivity of this IFN was about 10 ct/min/IFN unit.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-2-285
1983-02-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/2/JV0640020285.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-2-285&mimeType=html&fmt=ahah

References

  1. Adolf G. R., Swetly P. 1979; Interferon production by human lymphoblastoid cells is stimulated by inducers of Friend cell differentiation. Virology 99:158–166
    [Google Scholar]
  2. Baker P. N., Morser J., Burke D. C. 1980; Effects of sodium butyrate on a human lymphoblastoid cell line (Namalwa) and its interferon production. Journal of Interferon Research 1:71–77
    [Google Scholar]
  3. Cabrer B., Taira H., Broeze R. J., Kempe T. D., Williams K., Slattery E., Konisberg W. H., Lengyel P. 1979; Structural characteristics of interferon from mouse Ehrlich ascites tumor cells. Journal of Biological Chemistry 254:3681–3684
    [Google Scholar]
  4. Cachard A., De Maeyer-Guignard J. 1981; Enhancement of mouse interferon production by combined treatment of C 243 cells with butyric acid and theophylline. Annales de Virologie 132:307–312
    [Google Scholar]
  5. Chelbi-Alix M. K., Thang M. N. 1981; Separation of molecular species of human leucocyte and mouse interferon by polynucleotide affinity chromatography. Methods in Enzymology 78:582–591
    [Google Scholar]
  6. Davey M. W., Huang J. W., Sulkowski E., Carter W. A. 1976; Hydrophobic interaction of human, mouse and rabbit interferons with immobilized hydrocarbons. Journal of Biological Chemistry 251:7620–7625
    [Google Scholar]
  7. Demaeyer-Guignard J., Thang M. N., De Maeyer E. 1977; Binding of mouse interferon to polynucleotides. Proceedings of the National Academy of Sciences of the United States of America 74:3787–3790
    [Google Scholar]
  8. Johnston M. D. 1980; Enhanced production of interferon from human lymphoblastoid (Namalwa) cells pretreated with sodium butyrate. Journal of General Virology 50:191–194
    [Google Scholar]
  9. Kawakita M., Cabrer B., Taira H., Rebello M., Slattery E., Weidel H., Lengyel P. 1978; Purification of interferon from mouse Ehrlich ascites tumor cells. Journal of Biological Chemistry 253:598–602
    [Google Scholar]
  10. Knight E., Hunkapiller M. W. 1981; Characterisation of radioactive human fibroblast-derived beta interferon synthesized in vivo. Journal of Interferon Research 1:297–303
    [Google Scholar]
  11. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  12. Mecs I. 1973; Regulation of interferon production in vitro and in vivo by influencing the adenylate cyclase system. Acta microbiologica Academiae scientiarum hungaricae 20:42
    [Google Scholar]
  13. Reizin F. N., Roikhel V. M., Chumakov M. P. 1975; The influence of substances changing the intracellular concentration of cyclic adenosine 3′5′-monophosphate on interferon synthesis in chick embryo cell cultures. Archives of Virology 49:307–315
    [Google Scholar]
  14. Slattery E., Taira H., Broeze R., Lengyel P. 1980; Mouse interferon: production by Ehrlich ascites tumour cells infected with Newcastle disease virus and its enhancement by theophylline. Journal of General Virology 49:91–96
    [Google Scholar]
  15. Spector T. 1978; Refinement of the Coomassie blue method of protein quantitation. Analytical Biochemistry 86:142–146
    [Google Scholar]
  16. Studier F. W. 1973; Analysis of bacteriophage T7: early RNAs and proteins on slab gels. Journal of Molecular Biology 79:237–248
    [Google Scholar]
  17. Taira H., Broeze R. J., Jayaram B. M., Lengyel P., Hunkapiller M. W., Hood L. E. 1980; Mouse interferons: amino terminal amino acid sequences of various species. Science 207:528–530
    [Google Scholar]
  18. Yonehara S., Iwakura Y., Kawade Y. 1980; Rapid purification of mouse L cell interferon labeled with radioactive amino acid by immune precipitation. Virology 100:125–129
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-2-285
Loading
/content/journal/jgv/10.1099/0022-1317-64-2-285
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error