1887

Abstract

SUMMARY

The 7.2 kb genomic DNA of bovine papillomavirus type 3 (BPV-3) was molecularly cloned using its unique RI site, and the 7.3 kb genome of BPV-4 was cloned using its single HI site. The viral genomes were compared by liquid hybridization, Southern blot hybridization and heteroduplex mapping. Low stringency hybridization conditions revealed that the genomes are colinear but the sequences are extensively mismatched. The relative alignment of the restriction endonuclease maps of the two viral genomes has been determined. It was found that the genomes as linearized for cloning are out of phase by 1.7 kb, so that the single RI site of BPV-3 appears to coincide with the BPV-4 RI site at 0.22 map units. It is concluded that the genomes of BPV-3 and BPV-4, both of which cause true epithelial warts, share the same physical organization but exhibit sequence divergence.

Keyword(s): BPV , genome and homology
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-12-2771
1983-12-01
2021-10-27
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/12/JV0640122771.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-12-2771&mimeType=html&fmt=ahah

References

  1. Bonner T. I., Brenner D. J., Neufeld B. R., Britten R. J. 1973; Reduction in the rate of DNA reassociation by sequence divergence. Journal of Molecular Biology 81:123–135
    [Google Scholar]
  2. Campo M. S., Coggins L. W. 1982; Molecular cloning of bovine papillomavirus genomes and comparison of their sequence homologies by heteroduplex mapping. Journal of General Virology 63:255–264
    [Google Scholar]
  3. Campo M. S., Moar M. H., Jarrett W. F. H., Laird H. M. 1980; A new papilloma virus associated with alimentary cancer in cattle. Nature, London 286:180–182
    [Google Scholar]
  4. Campo M. S., Moar M. H., Laird H. M., Jarrett W. F. H. 1981; Molecular heterogeneity and lesion site specificity of cutaneous bovine papillomaviruses. Virology 113:323–335
    [Google Scholar]
  5. Chen E. Y., Howley P. M., Levinson A. D., Seeburg P. H. 1982; The primary structure and genetic organization of the bovine papillomavirus type 1 genome. Nature, London 299:529–534
    [Google Scholar]
  6. Croissant O., Testaniere V., Orth G. 1982; Mise en evidence et localisation de regions conservees dans les génomes du papillomavirus humain la et du papillomavirus bovin par analyse d’heteroduplex au microscope électronique. Comptes rendus des seances de l ′Academie des Sciences 294:581–586
    [Google Scholar]
  7. Danos O., Katinka M., Yaniv M. 1982; Human papillomavirus la: a novel type of genome organization among Papovaviridae. EMBO Journal 1:231–236
    [Google Scholar]
  8. Danos O., Engel L. W., Chen E. Y., Yaniv M., Howley P. M. 1983; Comparative analysis of the human type la and bovine type 1 papillomavirus genomes. Journal of Virology 46:557–566
    [Google Scholar]
  9. Davis R. W., Simon M., Davidson N. 1971; Electron microscope heteroduplex methods for mapping regions of base sequence homology in nucleic acids. Methods in Enzymology 21:413–428
    [Google Scholar]
  10. Gissmann L., Diehl V., Schultz-Coulon H. J., Zur Hausen H. 1982; Molecular cloning and characterization of human papilloma virus DNA derived from a laryngeal papilloma. Journal of Virology 44:393–400
    [Google Scholar]
  11. Jarrett W. F. H., Murphy J., O’Neil B. W., Laird H. M. 1978; Virus-induced papillomas of the alimentary tract of cattle. International Journal of Cancer 22:323–328
    [Google Scholar]
  12. Jarrett W. F. H., Mcneil P. E., Laird H. M., O’Neil B. W., Murphy J., Campo M. S., Moar M. H. 1980; Papilloma viruses in benign and malignant tumors of cattle. In Viruses in Naturally Occurring Cancers pp 215–222 Edited by Essex M., Todaro G., Zur Hausen H. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Lancaster W. D., Olson C. 1978; Demonstration of two distinct classes of bovine papillomavirus. Virology 89:372–379
    [Google Scholar]
  14. Law M. F., Lancaster W. D., Howley P. M. 1979; Conserved polynucleotide sequences among the genomes of papillomaviruses. Journal of Virology 32:199–207
    [Google Scholar]
  15. Pfister H., Linz U., Gissmann L., Huchthausen B., Hoffmann D., Zur Hausen H. 1979; Partial characterization of a new type of bovine papillomavirus. Virology 96:1–8
    [Google Scholar]
  16. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  17. Sharp P. A., Pettersson U., Sambrook J. 1974; Viral DNA in transformed cells. I. A study of the sequences of adenovirus 2 DNA in a line of transformed rat cells using specific fragments of the viral genome. Journal of Molecular Biology 86:709–726
    [Google Scholar]
  18. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  19. Twigg A. J., Sherratt D. J. 1980; Trans-complementable copy number mutants of plasmid ColEl. Nature, London 283:216–218
    [Google Scholar]
  20. Wetmur J. G., Davidson N. 1968; Kinetics of renaturation of DNA. Journal of Molecular Biology 31:349–370
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-12-2771
Loading
/content/journal/jgv/10.1099/0022-1317-64-12-2771
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error