%0 Journal Article %A Weder, Dagmar %A Radsak, Klaus D. %T Induction of a Host-specific Chromatin-associated Glycopolypeptide by Human Cytomegalovirus %D 1983 %J Journal of General Virology, %V 64 %N 12 %P 2749-2761 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-64-12-2749 %K chromatin-associated glycopolypeptide %K cytomegalovirus %I Microbiology Society, %X SUMMARY Analysis of chromatin preparations from [3H]glucosamine-labelled human foreskin fibroblasts revealed that a chromatin-associated glycopolypeptide with the approximate mol. wt. 130000 (130K) is induced in response to either infection with human cytomegalovirus (HCMV) or serum treatment. Comparative limited proteolysis suggested that the [3H]glucosamine-labelled 130K polypeptides induced by these different stimuli were not identical. This observation was in contrast to results obtained by immunoprecipitation with antisera raised against the 130K glycopolypeptide from serum-induced cells which favoured a relatedness to the 130K polypeptide from virus infected cultures. Two-dimensional separation by isoelectric focusing and SDS–polyacrylamide gel electrophoresis subsequently showed that the 130K glycopolypeptide from serum-induced cells consists of two components, one of which is identical to the single component observed in samples from HCMV-infected cultures. Experiments on the effect of glycosylation inhibitors on DNA replication in HCMV-infected as well as in serum-induced cells support the view that the host-specific chromatin-associated glycopolypeptide may be involved in DNA replication in infected cells. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-12-2749