%0 Journal Article %A Gray, M. A. %A Austin, S. A. %A Clemens, M. J. %A Rodrigues, L. %A Pasternak, C. A. %T Protein Synthesis in Semliki Forest Virus-infected Cells is Not Controlled by Permeability Changes %D 1983 %J Journal of General Virology, %V 64 %N 12 %P 2631-2640 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-64-12-2631 %K Semliki Forest virus %K host cell shut-off %K cell permeability %I Microbiology Society, %X SUMMARY The uptake of the GTP analogue guanylyl(β,γ-methylene)diphosphonate (GppCH2p) is the same in Semliki Forest virus (SFV)-infected BHK cells as in mock-infected cells, in spite of the fact that protein synthesis is inhibited by GppCH2p more markedly in SFV-infected cells than in control cells. A possible explanation for this difference is that infected cells have a lower concentration of GTP and a lower ratio of GTP:GDP than uninfected cells, and the analogue may thus be a more effective competitive inhibitor of translation. We conclude that in this system, the difference between infected and uninfected cells lies not at the plasma membrane but within the cytoplasm. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-12-2631