1887

Abstract

SUMMARY

The genes of a field isolate of human rotavirus were cloned into pBR322. The strategy used involved polyadenylation of denatured double-stranded (ds) genomic RNA, followed by cDNA synthesis using reverse transcriptase. Oligo(dC) tails were added to the 3′ end of the single-stranded cDNA and then separated by alkaline agarose electrophoresis. Sized cDNA of both polarities were allowed to hybridize and inserted into the I site of pBR322. Transformations done with sized cDNA always resulted in the selection of hybrid plasmids carrying inserts with a size representative of the original cDNA. Four individual clones were selected for preliminary characterization. One clone has an insert of 1360 base pairs (bp) and corresponds to gene 6. The second clone has an insert of 1140 bp and corresponds to one of the genes in the triplet 7-8-9. The other two genes, with inserts of 780 and 660 bp, were identified as corresponding to dsRNA segments 10 and 11.

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1983-12-01
2021-10-20
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References

  1. Arias C. F., Lopez S., Espejo R. T. 1982; Gene protein products of SA11 simian rotavirus genome. Journal of Virology 41:42–50
    [Google Scholar]
  2. Aviv H., Leder P. 1972; Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid cellulose. Proceedings of the National Academy of Sciences, U,. S,. A 69:1408–1412
    [Google Scholar]
  3. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Research 7:1513–1523
    [Google Scholar]
  4. Both G. W., Bellamy A. R., Siegman I. J. 1982; A general strategy for cloning double-stranded RNA: nucleotide sequence of simian-11 rotavirus gene 8. Nucleic Acids Research 10:7075–7088
    [Google Scholar]
  5. Cashdollar L. W., Esparza J., Hudson G. R., Chmelo R., Lee P. W. K., Joklik W. K. 1982; Cloning the double-stranded RNA genes of reovirus: sequence of the cloned S2 gene. Proceedings of the National Academy of Sciences, U. S. A 79:7644–7648
    [Google Scholar]
  6. Cohen J. 1977; Ribonucleic acid polymerase activity associated with purified calf rotavirus. Journal of General Virology 36:395–402
    [Google Scholar]
  7. Cohen J., Laporte J., Charpiliene A., Scherrer R. 1979; Activation of rotavirus RNA polymerase by calcium chelation. Archives of Virology 60:177–186
    [Google Scholar]
  8. Dyall-Smith M. L., Holmes I. H. 1981a; Gene-coding assignments of rotavirus double-stranded RNA segments 10 and 11. Journal of Virology 38:1099–1103
    [Google Scholar]
  9. Dyall-Smith M. L., Holmes I. H. 1981b; Comparison of rotavirus polypeptides by limited proteolysis: close similarity of certain polypeptides of different strains. Journal of Virology 40:720–728
    [Google Scholar]
  10. Erickson B. L., Graham D. Y., Mason B. B., Estes M. K. 1982; Identification, synthesis, and modification of simian rotavirus SA 11 polypeptides in infected cells. Journal of Virology 42:825–839
    [Google Scholar]
  11. Esparza J., Gil F. 1978; A study on the ultrastructure of human rotavirus. Virology 91:141–150
    [Google Scholar]
  12. Esparza J., Gorziglia M., Gil F., Romer H. 1980; Multiplication of human rotavirus in cultured cells: an electron microscopic study. Journal of General Virology 47:461–472
    [Google Scholar]
  13. Espejo R. T., Avendano L. F., Munoz O., Romero P., Enternod J. G., Lopez S., Moncaya J. 1980; Comparison of human rotaviruses isolated in Mexico City and Santiago, Chile by electrophoretic migration of their double-stranded RNA genome segments. Infection and Immunity 30:342–348
    [Google Scholar]
  14. Espejo R. T., Lopez S., Arias C. 1981; Structural polypeptides of simian rotavirus SA 11 and the effect of trypsin. Journal of Virology 37:156–160
    [Google Scholar]
  15. Flewett T. H., Woode G. N. 1978; The rotaviruses. Archives of Virology 57:1–23
    [Google Scholar]
  16. Gaillard R. K., Joklik W. K. 1982; Quantitation of the relatedness of reovirus serotypes 1, 2 and 3 at the gene level. Virology 123:152–164
    [Google Scholar]
  17. Gething M. J., Bye J., Skehel J., Waterfield M. 1980; Cloning and DNA sequence of double-stranded copies of haemagglutinin genes from H2 and H3 strains elucidates antigenic shift and drift in human influenza virus. Nature, London 287:301–306
    [Google Scholar]
  18. Goldberg M. L., Lefton R. P., Stark G. R., Williams J. G. 1979; Isolation of specific RNAs using DNA covalently linked to diazobenzyloxymethyl-cellulose on paper. Methods in Enzymology 68:206–220
    [Google Scholar]
  19. Holmes I. H. 1979; Viral gastroenteritis. Progress in Medical Virology 25:1–36
    [Google Scholar]
  20. Imai M., Richardson M. A., Ikegami N., Shatkin A. J., Furuichi Y. 1983; Molecular cloning of double-stranded RNA virus genomes. Proceedings of the National Academy of Sciences, U,. S,. A 80:373–377
    [Google Scholar]
  21. Kalica A. R., Sereno M. M., Wyatt R. G., Mebus C. A., Chanock R. M., Kapikian A. Z. 1978; Comparison of human and animal rotavirus strains by gel electrophoresis of viral RNA. Virology 87:247–255
    [Google Scholar]
  22. Kalica A. R., Greenberg H. B., Espejo R. T., Flores J., Wyatt R. G., Kapikian A. Z., Chanock R. M. 1981; Distinctive ribonucleic acid patterns of human rotavirus subgroups 1 and 2. Infection and Immunity 33:958–961
    [Google Scholar]
  23. Killen H. M., Dimmock N. J. 1982; Identification of a neutralization-specific antigen of a calf rotavirus. Journal of General Virology 62:297–311
    [Google Scholar]
  24. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  25. Mccrae M. A., Joklik W. K. 1978; The nature of the polypeptide encoded by each of the 10 double-stranded RNA segments of reovirus type 3. Virology 89:578–593
    [Google Scholar]
  26. Mccrae M. A., Mccorquodale J. G. 1982a; The molecular biology of rotaviruses. II. Identification of the protein-coding assignments of calf rotavirus genome RNA species. Virology 117:435–443
    [Google Scholar]
  27. Mccrae M. A., Mccorquodale J. G. 1982b; Molecular biology of rotaviruses. IV. Molecular cloning of the bovine rotavirus genome. Journal of Virology 44:1076–1079
    [Google Scholar]
  28. Mcdonnell M. W., Simon M. N., Studier F. W. 1977; Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels. Journal of Molecular Biology 110:119–146
    [Google Scholar]
  29. Maizel J. V. Jr 1971; Polyacrylamide gel electrophoresis of viral proteins. Methods in Virology 5:179–246
    [Google Scholar]
  30. Matsuno S., Inouye S. 1983; Purification of an outer capsid glycoprotein of neonatal calf diarrhoea virus and preparation of its antisera. Infection and Immunity 39:155–158
    [Google Scholar]
  31. Novo E., Esparza J. 1981; Composition and topography of structural polypeptides of bovine rotavirus. Journal of General Virology 56:325–335
    [Google Scholar]
  32. Paterson B. M., Roberts B. E., Kuff E. L. 1977; Structural gene identification and mapping by DNA-mRNA hybrid arrested cell-free translation. Proceedings of the National Academy of Sciences, U,. S,. A 74:4370–4374
    [Google Scholar]
  33. Rodger S. M., Holmes I. H. 1979; Comparison of the genomes of simian, bovine, and human rotaviruses by gel electrophoresis and detection of genomic variation among bovine isolates. Journal of Virology 30:839–846
    [Google Scholar]
  34. Rodger S. M., Bishop R. F., Birch C., Mclean B., Holmes I. H. 1981; Molecular epidemiology of human rotaviruses in Melbourne, Australia, from 1973 to 1979, as determined by electrophoresis of genome ribonucleic acid. Journal of Clinical Microbiology 13:272–278
    [Google Scholar]
  35. Roseto A., Escaig J., Delain E., Cohen J., Scherrer R. 1979; Structure of rotaviruses as studied by the freezedrying technique. Virology 98:471–475
    [Google Scholar]
  36. Roychoudhury R., Wu R. 1980; Terminal transferase-catalyzed addition of nucleosides to the 3′ end of DNA. Methods in Enzymology 65:43–62
    [Google Scholar]
  37. Sato K., Inaba Y., Shinozaki T., Fujii R., Matumoto M. 1981; Isolation of human rotavirus in cell cultures. Archives of Virology 69:155–160
    [Google Scholar]
  38. Sippel A. E. 1973; Purification and characterization of adenosine triphosphate: ribonucleic acid adenyltransfer-ase from Escherichia coli. European Journal of Biochemistry 37:31–40
    [Google Scholar]
  39. Smith M. L., Lazdins I., Holmes I. H. 1980; Coding assignments of double-stranded RNA segments of SA 11 rotavirus established by in vitro translation. Journal of Virology 33:976–982
    [Google Scholar]
  40. Smith R. E., Zweerink H. J., Joklik W. K. 1969; Polypeptide components of virions, top component and cores of reovirus type 3. Virology 39:791–810
    [Google Scholar]
  41. Southern E. M. 1975; Detection of specific sequences among DNA fragments separated by gel electrophoresis. Journal of Molecular Biology 98:503–517
    [Google Scholar]
  42. Spencer E. G., Avendano L. F., Garcia B. I. 1983; Analysis of human rotavirus mixed electropherotypes. Infection and Immunity 39:569–574
    [Google Scholar]
  43. Viera de Torres B., Mazzali de Ilja R., Esparza J. 1978; Epidemiological aspects of rotavirus infection in hospitalized Venezuelan children with gastroenteritis. American Journal of Tropical Medicine and Hygiene 27:567–572
    [Google Scholar]
  44. Vogelstein B., Gillespie D. 1979; Preparative and analytical purification of DNA from agarose. Proceedings of the National Academy of Sciences, U,. S,. A 76:615–619
    [Google Scholar]
  45. Wyatt R. G., James W. D., Bohl E. H., Theil K. W., Saif L. V., Kalica A. R., Greenberg H. B., Kapikian A. Z., Chanock R. M. 1980; Human rotavirus type 2: cultivation in vitro. Science 207:189–191
    [Google Scholar]
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