%0 Journal Article %A Archard, L. C. %T Synthesis of Full-length, Virus Genomic DNA by Nuclei of Vaccinia-infected HeLa Cells %D 1983 %J Journal of General Virology, %V 64 %N 12 %P 2561-2575 %@ 1465-2099 %R https://doi.org/10.1099/0022-1317-64-12-2561 %K DNA synthesis %K vaccinia virus %K nuclei %I Microbiology Society, %X SUMMARY It is well established that vaccinia virus infection induces the synthesis of virus-specific DNA in cytoplasmic ‘factories’, which are sites of virus-specific transcription. The present study demonstrates that vaccinia virus-specific DNA is synthesized also in the nuclei of infected cells with a similar time course. Direct observation and radiolabelling confirm the integrity of isolated nuclei. Reconstitution experiments and inhibitor studies demonstrate that virus-induced DNA is synthesized de novo within nuclei and does not result from cytoplasmic contamination. Cell-specific DNA synthesis is inhibited completely after infection and nuclei of infected cells then synthesize DNA which co-sediments with virus genomic DNA in denaturing gradients. Restriction endonuclease cleavage and hybridization with a virus-specific probe indicate that this is full-length, virus genomic DNA. The biological implications of this are discussed. %U https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-12-2561