1887

Abstract

Summary

A physical map has been constructed for bovine parvovirus (BPV) replicative form (RF) DNA synthesized . Analysis of restriction enzyme digestion products for 12 enzymes by neutral gel electrophoresis allowed the mapping of 26 cleavage sites between the 3′ and 5′ termini of the RF DNA. Cleavage sites for these enzymes were not detected within the 3′-terminal hairpin. dIII, I, I, II and II did not cleave BPV DNA, while 12 other enzymes cleaved the genome at one to four sites. Eleven additional enzymes were shown to cleave the genome at 8 to 20 sites. Comparison of the restriction site locations for BPV with those of other autonomous parvoviruses suggests that there may be conservation within the genome of certain restriction sites.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-11-2521
1983-11-01
2019-10-14
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-11-2521
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error