1887

Abstract

SUMMARY

The PC12 cell line, which is derived from a rat pheochromocytoma and possesses a number of ‘differentiated’ neuronal properties, was used to characterize the effect of herpes simplex virus type 1 (HSV-1) infection on the uptake and release of catecholamines. Both the uptake of [H]norepinephrine and the content and K-induced release of endogenous catecholamines were remarkably preserved during the course of productive infection. HSV-1 infection is thus selective in its effects on the host cell and certain specialized functional properties may be retained in the face of otherwise profound metabolic alterations.

Keyword(s): catecholamines , HSV-1 and neurons
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/content/journal/jgv/10.1099/0022-1317-64-11-2505
1983-11-01
2024-04-25
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References

  1. Bornstein M. B. 1958; Reconstituted rat tail collagen used as a substrate for tissue culture on coverslips in Maximow slides and roller tubes. Laboratory Investigation 1:134–137
    [Google Scholar]
  2. Esiri M. M. 1982; Herpes simplex encephalitis an immunohistological study of the distribution of viral antigen within the brain. Journal of Neurological Sciences 54:209–226
    [Google Scholar]
  3. Greene L. A., Rein G. 1977a; Release, storage and uptake of catecholamines by a clonal line of nerve growth factor (NGF) responsive pheochromocytoma cells. Brain Research 129:247–263
    [Google Scholar]
  4. Greene L. A., Rein G. 1977b; Release of [3H]norepinephrine from a clonal line of pheochromocytoma cells (PC12) by nicotinic cholinergic stimulation. Brain Research 138:521–528
    [Google Scholar]
  5. Greene L. A., Tischler A. S. 1976; Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proceedings of the National Academy of Sciences, U,. S,. A 73:2424–2428
    [Google Scholar]
  6. Mobley W. C., Schenker A., Shooter E. M. 1976; Characterization and isolation of proteolytically modified nerve growth factor. Biochemistry 15:5543–5552
    [Google Scholar]
  7. Price R. W., Rubenstein R., Khan A. 1982; Herpes simplex virus infection of isolated autonomic neurons in culture viral replication and spread in a neuronal network. Archives of Neurology 71:127–140
    [Google Scholar]
  8. Ritchie A. K. 1979; Catecholamine secretion in a rat pheochromocytoma cell line: two pathways for calcium entry. Journal of Physiology 286:541–561
    [Google Scholar]
  9. Rubenstein R., Price R. W. 1983; Early inhibition of acetylcholinesterase and choline acetyltransferase activity in herpes simplex virus type 1 infection of PC12 cells. Journal of Neurochemistry (in press)
    [Google Scholar]
  10. Rudy B., Kirschenbaum B., Greene L. A. 1982; Nerve growth factor-induced increase in saxitoxin binding to rat PCI2 pheochromocytoma cells. Journal of Neuroscience 2:1405–1411
    [Google Scholar]
  11. Saller C. F., Zigmond M. J. 1978; A radioenzymatic assay for catecholamines and dihydroxyphenylacetic acid. Life Sciences 23:1117–1130
    [Google Scholar]
  12. Spear P. G., Roizman B. 1980; Herpes simplex viruses. In DNA Tumor Viruses part 2: Molecular Biology of Tumor Viruses 2nd ed pp 615–745 Edited by Tooze J. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  13. Stevens J. G. 1975; Latent herpes simplex virus and the nervous system. Current Topics in Microbiology and Immunology 70:31–50
    [Google Scholar]
  14. Tsukamoto L. F., Price R. W. 1982; Interferon protects neurons in culture infected with vesicular stomatitis and herpes simplex viruses. Journal of Neurological Sciences 56:115–128
    [Google Scholar]
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