1887

Abstract

SUMMARY

By indirect immunofluorescence microscopy (IIF) we have localized glycoprotein D (gD) of herpes simplex virus-infected cells to the vinculin-containing junctional areas and to the focal adhesion sites on the ventral surface of the cells. Double-staining IIF showed that gD and vinculin co-distributed in the infected cells and treatment of the infected cells 4 h post-infection with rabbit antibodies to gD prevented attachment of the cells to the growth substratum. Our results therefore support the hypothesis that gD plays a central role in the social behaviour of infected cells.

Keyword(s): focal adhesion , gD , HSV and vinculin
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/content/journal/jgv/10.1099/0022-1317-64-11-2499
1983-11-01
2021-10-19
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References

  1. Bauke R. B., Spear P. G. 1979; Membrane proteins specified by herpes simplex viruses. V. Identification of an Fc-binding glycoprotein. Journal of Virology 32:779–789
    [Google Scholar]
  2. Eberle R., Courtney R. J. 1980; gA and gB glycoproteins of herpes simplex virus type 1: two forms of a single polypeptide. Journal of Virology 36:665–675
    [Google Scholar]
  3. Eisenberg R. J., Hydran-stern E., Cohen G. H. 1979; Structural analysis of precursor and product forms of type-common envelope glycoprotein D (CP-1 antigen) of herpes simplex virus type 1. Journal of Virology 31:608–620
    [Google Scholar]
  4. Geiger B. 1979; A 130K protein from chicken gizzard: its localization at the termini of microfilament bundles in cultured chicken cells. Cell 18:193–205
    [Google Scholar]
  5. Geiger B., Schmid E., Franke W. W. 1983; Spatial distribution of proteins specific for desmosomes and adherence junctions in epithelial cells demonstrated by double immunofluorescence microscopy. Differentiation 23:189–205
    [Google Scholar]
  6. Hoggan M. D., Roizman B., Turner T. B. 1960; The effect of the temperature of incubation on the spread of herpes simplex virus in an immune environment in cell culture. Journal of Immunology 84:152–159
    [Google Scholar]
  7. Honess R. W., Roizman B. 1975; Proteins specified by herpes simplex virus. XIII. Glycosylation of viral polypeptides. Journal of Virology 16:1308–1326
    [Google Scholar]
  8. Johnson D. C., Spear P. G. 1983; O-linked oligosaccharides are acquired by herpes simplex virus glycoproteins in the Golgi apparatus. Cell 32:987–997
    [Google Scholar]
  9. Lehto V.-P., Hovi T., Vartio T., Badley R. A., Virtanen I. 1982; Reorganization of cytoskeletal and contractile elements during transition of human monocytes into adherent macrophages. Laboratory Investigation 47:391–399
    [Google Scholar]
  10. Manservigi R., Spear P. G., Buchan A. 1977; Cell fusion induced by herpes simplex virus is promoted and suppressed by different viral glycoproteins. Proceedings of the National Academy of Sciences, U,. S,. A 74:3913–3917
    [Google Scholar]
  11. Norrild B., Pedersen B. 1982; The effect of tunicamycin on the synthesis of herpes simplex virus type 1 glycoproteins and on their expression on the cell surface. Journal of Virology 43:395–402
    [Google Scholar]
  12. Norrild B., Shore S. L., Cromeans T. L., Nahmias A. J. 1980; Participation of three major glycoprotein antigens of herpes simplex virus type 1 early in the infectious cycle as determined by antibody-dependent cell- mediated cytotoxicity. Infection and Immunity 28:38–44
    [Google Scholar]
  13. Norrild B., Virtanen L., Pedersen B., Pereira L. 1983; Processing of HSV-1 glycoproteins in human fibroblasts and Vero-cells. Archives of Virology (in press)
    [Google Scholar]
  14. Oesch B., Birchmeier W. 1982; New surface component of fibroblasts’ focal contacts identified by a monoclonal antibody. Cell 31:671–679
    [Google Scholar]
  15. Olofsson S., Norrild B., Andersen A. B., Pereira L., Jeansson S., Lycke E. 1983; Populations of herpes simplex virus glycoprotein gC with and without affinity for the jV-acetylgalactosamine specific lectin of Helix pomatia . Archives of Virology 76:25–38
    [Google Scholar]
  16. Pereira L., Klassen T., Baringer J. R. 1980; Type common and type specific monoclonal antibody to herpes simplex virus 1. Infection and Immunity 29:724–732
    [Google Scholar]
  17. Pereira L., Dondero D., Roizman B. 1982; Herpes simplex virus glycoprotein gA/gB: evidence that the infected VERO cell products comap and arise by proteolysis. Journal of Virology 44:88–97
    [Google Scholar]
  18. Roizman B. 1971; Herpesviruses, membranes and social behaviour of infected cells. In Proceedings of the 3rd International Symposium on Applied and Medical Virology pp 37–72 Edited by Sanders M. St. Louis: W. H. Green;
    [Google Scholar]
  19. Ruyechan W. T., Morse L. S., Knipe D. M., Roizman B. 1979; Molecular genetics of herpes simplex virus. II. Mapping of the major viral glycoproteins and of the genetic loci specifying the social behavior of infected cells. Journal of Virology 29:677–697
    [Google Scholar]
  20. Sarmiento M., Haffey M., Spear P. G. 1979; Membrane proteins specified by herpes simplex viruses. III. Role of glycoprotein VP 7 (B2) in virion infectivity. Journal of Virology 29:1149–1158
    [Google Scholar]
  21. Spear P. G. 1976; Membrane proteins specified by herpes simplex viruses. I. Identification of four glycoprotein precursors and their products in type 1-infected cells. Journal of Virology 17:991–1008
    [Google Scholar]
  22. Vestergaard B. F., Norrild B. 1979; Crossed immuno-electrophoretic analysis and viral neutralizing activity of five monospecific antisera against five different herpes simplex virus glycoproteins. In Oncogenesis and Herpesviruses HI pp 225–234 Edited by De The G. Henle W., Rapp F. Lyon: IARC Scientific Publications, No. 24;
    [Google Scholar]
  23. Virtanen I., Vartio T., Badley R. A., Lehto V.-P. 1982; Fibronectin in adhesion, spreading and cytoskeletal organization of cultured fibroblasts. Nature, London 298:660–663
    [Google Scholar]
  24. Wenske E. A., Bratton M. W., Courtney R. J. 1982; Endo-/i-acetylglucosaminidase H sensitivity of precursors to herpes simplex virus type 1 glycoproteins gB and gC. Journal of Virology 44:241–248
    [Google Scholar]
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