1887

Abstract

SUMMARY

Cultures of hybrid cells secreting monoclonal antibodies to potato virus Y (PVY) were produced by fusing a non-secreting FO myeloma cell line with spleen cells from BALB/c mice immunized with an isolate of the tobacco veinal necrosis strain group (PVY 605). Monoclonal antibodies were obtained which reacted with common antigenic determinants of 24 isolates belonging to the homologous (PVY), common (PVY) and stipple streak (PVY) strain groups of PVY. Other antibodies were either strictly specific to PVY isolates or of intermediate specificity. In enzyme-linked immunosorbent assay (ELISA) for the detection of PVY viruses, a preparation of monoclonal antibody to a common antigenic determinant gave more uniform reactions than polyclonal antibodies from rabbit serum. Enzyme conjugates of monoclonal antibody preparations could be used highly diluted without loss of activity in ELISA, thereby decreasing the background caused by non-specific reactions.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-11-2471
1983-11-01
2024-12-05
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/11/JV0640112471.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-11-2471&mimeType=html&fmt=ahah

References

  1. Al moudallal Z., Briand J. P., Van regenmortel M. H. V. 1982; Monoclonal antibodies as probes of the antigenic structure of tobacco mosaic virus. EMBO Journal 8:1005–1010
    [Google Scholar]
  2. Bartels R. 1957; Serologische Differenzierungsversuche mit Stämmen des Kartoffel-Y-Virus. In Proceedings of the 3rd Conference on Potato Virus Diseases (Lisse-Wageningen, 24–28 June, 1957)
    [Google Scholar]
  3. Bawden F. C., Kassanis B. 1951; Serologically related strains of potato virus Y that are not mutually antagonistic in plants. Annals of Applied Biology 38:402–410
    [Google Scholar]
  4. Briand J. P., AL Moudallal Z., Van Regenmortel M. H. V. 1982; Serological differentiation of tobamoviruses by means of monoclonal antibodies. Journal of Virological Methods 5:293–300
    [Google Scholar]
  5. Calvert E. L., Cooper P., Mcclure J. 1980; An aphid transmitted strain of PVYC recorded in potatoes in Northern Ireland. Record of Agricultural Research (N. Ireland) 28:63–73
    [Google Scholar]
  6. Clark M. F., Adams A. N. 1977; Characteristics of the microplate method of enzyme–linked immunosorbent assay for the detection of plant viruses. Journal of General Virology 34:475–483
    [Google Scholar]
  7. De Bokx J. A., Huttinga H. 1981; Potato virus Y. Commonwealth Mycological Institute/Association of Applied Biologists Descriptions of Plant Viruses No. 242
    [Google Scholar]
  8. De Bokx J. A., Kratchanova B., Maat D. Z. 1975; Some properties of a deviating strain of potato virus Y. Potato Research 18:38–51
    [Google Scholar]
  9. Dietzgen R. G., Sander E. 1981; Production of monoclonal antibodies against a plant virus. In Abstracts of the 5th International Congress of Virology (Strasbourg)
    [Google Scholar]
  10. Dietzgen R. G., Sander E. 1982; Monoclonal antibodies against a plant virus. Archives of Virology 74:197–204
    [Google Scholar]
  11. Fazekas De ST., Groth S., Scheidegger D. 1980; Production of monoclonal antibodies: strategy and tactics. Journal of Immunological Methods 35:1–21
    [Google Scholar]
  12. Fribourg C. E., Nakashima J. 1982; A PVY strain that does not induce local lesions in clone A6. In Proceedings of CIP Decennial Anniversary Congress ‘Research for the Potato in the Year 2000’ pp 86–87 Lima: The International Potato Center;
    [Google Scholar]
  13. Gerhard W., Kendal A. P. 1982; Characterization, structure and variant analysis of viruses with particular reference to influenza. In Monoclonal Antibodies in Clinical Medicine pp 253–276 Edited by McMichael J., Fabre J. W. New York & London: Academic Press;
    [Google Scholar]
  14. Gugerli P. 1978; The detection of two potato viruses by enzyme-linked immunosorbent assay (ELISA). Phytopathologische Zeitschrift 92:51–56
    [Google Scholar]
  15. Gugerli P. 1982; Improvements of routine tests for potato virus identification. In Proceedings of CIP Decennial Anniversary Congress ‘Research for the Potato in the Year 2000’ pp 91–92 Lima: The International Potato Center;
    [Google Scholar]
  16. Gugerli P., Fries P. 1981; Utilisation d’anticorps monoclonaux pour le diagnostic de viruses chez les plantes. Revue suisse de Viticulture, Arboriculture et Horticulture 13:323
    [Google Scholar]
  17. Kӧhler G., Milstein C. 1975; Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  18. Leiser R.-M., Richter J. 1979; Ein Beitrag zur Frage der Differenzierung von Stämmen des Kartoffel-Y-Virus. Archiv für Phytopathologie und Pflanzenschutz 15:289–298
    [Google Scholar]
  19. Maat D. Z., De Bokx J. A. 1978; Enzyme-linked immunosorbent assay (ELISA) for the detection of potato virus A and Y in potato leaves and sprouts. Netherlands Journal of Plant Pathology 84:167–173
    [Google Scholar]
  20. Salazar L. F. 1978; Simultaneous diagnosis of potato viruses by enzyme-linked immunosorbent assay (ELISA). In Abstracts of the 3rd International Congress of Plant Pathology (Munich), P 17
    [Google Scholar]
  21. Torrance L. 1981; Use of Clq enzyme-linked immunosorbent assay to detect plant viruses and their serologically different strains. Annals of Applied Biology 99:291–299
    [Google Scholar]
/content/journal/jgv/10.1099/0022-1317-64-11-2471
Loading
/content/journal/jgv/10.1099/0022-1317-64-11-2471
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error