1887

Abstract

SUMMARY

The specificity of the humoral response of chickens to avian tumour viruses (ATV) was investigated by reacting ATV-immune sera with Triton X-100 extracts of uninfected, infected and transformed chicken embryo fibroblasts. Analysis of these immune reactions by polyacrylamide gel electrophoresis revealed that avian leukosis virus-challenged and Rous sarcoma virus-challenged chickens recognized only two major cell surface antigens of 100000 and 29000 mol. wt. which were present on transformed and non-transformed virus-producing cells. No labelled antigens were precipitated from uninfected cells or transformed cells producing the envelope-defective mutant RSV(-). The antigens were shown to be related to the major envelope glycoproteins of the virus and to contain group-specific determinants common to ATV subgroups B and C. No group-specific determinants common to ATV subgroups A and B or subgroups A and C were detected. Chickens were found to have a strong antibody response to the 100000 and 29000 mol. wt. proteins prior to and during tumour rejection, even in the absence of neutralizing antibody to the challenge virus. No tumour-specific surface antigen distinct from the virion structural antigens was detected by any of the immune chicken sera on any of the transformed cells tested.

Keyword(s): antigens , ATV , cell surface and chicken
Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-11-2387
1983-11-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/11/JV0640112387.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-11-2387&mimeType=html&fmt=ahah

References

  1. Bauer H. 1974; Virion and tumor cell antigens of C-type RNA tumor viruses. Advances in Cancer Research 20:275–341
    [Google Scholar]
  2. Bauer H. 1978 In Avian RNA Tumour Viruses pp 252–267 Edited by Barlatti S. J., De Girli-Morghen C. Padua: Piccin;
    [Google Scholar]
  3. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  4. Brugge J. S., Erikson E., Erikson R. L. 1978; Antibody to virion structural proteins in mammals bearing avian sarcoma virus-induced tumors. Virology 84:429–433
    [Google Scholar]
  5. Brugge J. S., Collett M. S., Siddiqui A., Marczynska B., Deinhardt F., Erikson R. L. 1979; Detection of the viral sarcoma gene product in cells infected with various strains of avian sarcoma virus and of a related protein in uninfected chicken cells. Journal of Virology 29:1196–1203
    [Google Scholar]
  6. Buchhagen D. L., Hanafusa H. 1978; Intracellular precursors to the major glycoproteins of the avian oncoviruses in chicken embryo fibroblasts. Journal of Virology 25:845–851
    [Google Scholar]
  7. Collett M. S., Erikson E., Erkson R. L. 1979; Structural analysis of avian sarcoma virus transforming protein sites of phosphorylation. Journal of Virology 29:770–781
    [Google Scholar]
  8. Collins J. J., Montelaro R. C., Denny T. P., Ishizaki R., Langlois A. J., Bolognesi D. P. 1978; Normal chicken cells (chf) express a surface antigen which cross-reacts with determinants of the major envelope glycoprotein (gp85) of avian myeloblastosis virus. Virology 86:205–216
    [Google Scholar]
  9. Dougherty R. M., Simons P. J., Chesterman F. C. 1963; Biological properties of three variants of Rous sarcoma virus. Journal of the National Cancer Institute 31:1285–1307
    [Google Scholar]
  10. Ellwart-Tschurtz M., Kurth R. 1981; Demonstration of avian sarcoma virus coded pp60src in vivo and anti-pp60src immune response in chickens. Journal of Virology 39:950–953
    [Google Scholar]
  11. England J. M., Bolognesi D. P., Bernhard D., Halpern M. S. 1977; Evidence that a precursor glycoprotein is cleaved to yield the major glycoprotein of avian tumor virus. Journal of Virology 21:810–814
    [Google Scholar]
  12. Gahmberg C. G., Andersson L. C. 1977; Selective radioactive labeling of cell surface sialoglycoproteins by periodate-tritiated borohydride. Journal of Biological Chemistry 252:5888–5894
    [Google Scholar]
  13. Hall M. R., Qualtiere L. F., Meyers P. 1979; Cellular and humoral immune reactivity to tumor-associated antigens in chickens infected with Rous sarcoma virus. Journal of Immunology 123:1097–1105
    [Google Scholar]
  14. Halpern M. S., Friis R. R. 1978; Immunogenicity of the envelope glycoprotein of avian sarcoma virus. Proceedings of the National Academy of Sciences, U. S. A 75:1962–1966
    [Google Scholar]
  15. Hanafusa T., Miyamoto T., Hanafusa H. 1970; A type of chick embryo cell that fails to support formation of infectious RSV. Virology 40:55–64
    [Google Scholar]
  16. Kawai S., Hanafusa H. 1973; Isolation of defective mutant of avian sarcoma virus. Proceedings of the National Academy of Sciences, U. S. A 70:3493–3497
    [Google Scholar]
  17. Klemenz R., Diggelmann H. 1979; Extracellular cleavage of the glycoprotein precursor of Rous sarcoma virus. Journal of Virology 29:285–292
    [Google Scholar]
  18. Koldovsky P. 1969; Tumor specific transplantation antigen. Recent Results in Cancer Research 22:1–74
    [Google Scholar]
  19. Kurth R. M., Wyke J. A., Friis R. R., Rohrschneider L., Bauer H. 1976; The effect of transformation-defective avian oncornavirus mutants on tumor antigen expression. Journal of Supramolecular Structure 4:133–139
    [Google Scholar]
  20. Kurth R., Fenyo E. M., Klein E., Essex M. 1979; Cell surface antigens induced by RNA tumour viruses. Nature, London 279:197–201
    [Google Scholar]
  21. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  22. Meyers P. 1976; Antibody response to related leukosis viruses induced in chickens tolerant to an avian leukosis virus. Journal of the National Cancer Institute 56:381–386
    [Google Scholar]
  23. Meyers P., Dougherty R. M. 1971; Immunologic reactivity to viral antigens in chickens infected with avian leukosis viruses. Journal of the National Cancer Institute 46:701–711
    [Google Scholar]
  24. Meyers P., Qualtiere L. F. 1977; Tumor growth and antibodies after RSV challenge in normal chickens and in chickens congenitally infected with avian leukosis virus. Journal of Immunology 118:1541–1548
    [Google Scholar]
  25. Meyers P., Ritts G. D., Johnson D. R. 1976; Phytohemagglutinin-induced leukocyte blastogenesis in normal and avian leukosis virus-infected chickens. Cellular Immunology 27:140–146
    [Google Scholar]
  26. Morris R. E., Fritz R. R. 1976; Expression of virion and tumor specific antigens on the surface of chick embryo cells infected with strain MC29 avian leukosis virus. Cancer Research 36:414–419
    [Google Scholar]
  27. Phillips E. R., Perdue J. F. 1976; The expression and localization of surface neoantigens in transformed and untransformed cultured cells infected with avian tumor virus. Journal of Supramolecular Structure 4:27–44
    [Google Scholar]
  28. Phillips E. R., Perdue J. F. 1979; Failure to confirm evidence for a non-virion tumor-specific surface antigen in avian retrovirus-transformed cells. Journal of the National Cancer Institute 63:991–994
    [Google Scholar]
  29. Qualtiere L. F., Meyers P. 1979; A reexamination of humoral tolerance in chickens congenitally infected with an avian leukosis virus. Journal of Immunology 122:825–829
    [Google Scholar]
  30. Qualtiere L. F., Pearson G. R. 1979; Epstein-Barr virus induced membrane antigens: immunochemical characterization of Triton X-100 solubilized viral membrane antigens from EBV-superinfected Raji cells. International Journal of Cancer 23:808–817
    [Google Scholar]
  31. Rohrschneider L., Bauer H., Bolognesi D. P. 1975a; Group specific antigenic determinants of the large envelope glycoprotein of avian oncoma-viruses. Virology 67:234–241
    [Google Scholar]
  32. Rohrschneider L., Kurth R., Bauer H. 1975b; Biochemical characterization of tumor specific cell surface antigens (TSSA) of avian oncornavirus transformed cells. Virology 66:481–491
    [Google Scholar]
  33. Schlesinger J. J. 1976; Formation of an infectious virus-antibody complex with Rous sarcoma virus and antibodies directed against the major virus glycoprotein. Journal of Virology 17:1063–1076
    [Google Scholar]
  34. Van Eldik L. J., Paulson J. C., Green R. W., Smith R. E. 1978; The influence of carbohydrate on the antigenicity of the envelope glycoprotein of avian myeloblastosis virus and B77 avian sarcoma virus. Virology 86:193–204
    [Google Scholar]
  35. Vogt P. K., Ishizaki R. 1965; Reciprocal patterns of genetic resistance to avian tumor viruses in two lines of chickens. Virology 26:664–672
    [Google Scholar]
  36. Wainberg M. A., Phillips E. R. 1976; Immunity against avian sarcomas: a review. Israel Journal of Medical Sciences 12:388–406
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-11-2387
Loading
/content/journal/jgv/10.1099/0022-1317-64-11-2387
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error