1887

Abstract

SUMMARY

Detailed antigenic analysis using a panel of monoclonal antibodies was carried out on the haemagglutinin antigen of 53 influenza B viruses isolated from an epidemic in a single school. Thirteen distinguishable antigenic groupings of influenza B viruses could be detected but 26 of the viruses were in two groups (III and IV) which co-existed during the entire epidemic. Antigenically distinguishable influenza B viruses were isolated from an epidemic in a second nearby school. Influenza B viruses isolated from the two schools could be further distinguished by different electrophoretic mobilities of NS1 polypeptides and of genes 1, 2, 3 and 6, whereas viruses from a single school epidemic were very closely related as regards these biochemical characteristics. The findings are consistent with the hypothesis that the outbreak was initiated by a single individual who excreted antigenic mutants of which predominantly two spread and coexisted during the epidemic, although the additional occurrence of random mutations during the evolution of the epidemic cannot be excluded.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-11-2367
1983-11-01
2022-01-26
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/11/JV0640112367.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-11-2367&mimeType=html&fmt=ahah

References

  1. Brand C.M., Palese P. 1980; Sequential passage of influenza virus in embryonated eggs or tissue culture-emergence of mutants. Virology 107:424–433
    [Google Scholar]
  2. Brand C. M., Stealy V. M., Rowe J. 1977; Peptide mapping of 125I-labelled influenza virus proteins. Matrix proteins as markers in recombination. Journal of General Virology 36:385–394
    [Google Scholar]
  3. Ming C. C., Tian S. F., Ren G. F., Zhang T. M., Zhang L. X., Liu G. Q. 1982; Occurrence of temperature sensitive influenza A viruses in nature. Journal of Virology 41:353–359
    [Google Scholar]
  4. De Jong W. W., Zweers A., Cohen L. H. 1978; Influence of single amino acid substitutions on electrophoretic mobility of sodium dodecyl sulfate-protein complexes. Biochemical and Biophysical Research Communications 82:532–539
    [Google Scholar]
  5. Dimmock N. J., Carver A. S., Webster R. G. 1980; Categorising of nucleoprotein and matrix proteins from type A influenza viruses by peptide mapping. Virology 103:350–356
    [Google Scholar]
  6. Haaheim L. R. 1980; Haemagglutination inhibition antibodies in human sera to an antigenic mutant of influenza A/Texas/1/77 (H3N2) virus obtained in vitro . Acta pathologica et microbiologica scandinavica 88:351
    [Google Scholar]
  7. Holland J., Spindler K., Hordyski F., Grabau E., Nichol S., Van De Pol S. 1982; Rapid evolution of RNA genomes. Science 215:1577–1585
    [Google Scholar]
  8. Hoskins T. W., Davies J. R., Smith A. J., Miller C. L., Allchin A. 1979; Assessment of inactivated influenza A vaccine after three outbreaks of influenza A at Christ’s Hospital. Lancet i:33–35
    [Google Scholar]
  9. Hugentobler A. L., Schild G. C., Oxford J. S. 1981; Differences in the electrophoretic migration rates of polypeptides and RNAs of recent isolates of influenza B viruses. Archives of Virology 69:197–207
    [Google Scholar]
  10. Kendal A. P., Joseph J. M., Kobayashi G., Nelson D., Reyes C. R., Ross M. R., Sarandria J. L., White R. W., Woodall D. F., Nobel G. R., Dowdle W. R. 1979; Laboratory based surveillance of influenza virus in the United States during the winter of 1977–78. I. Periods of prevalence of H1N1 and H3N2 influenza strains, their relative rates of isolation in different age groups and detection of antigenic variants. American Journal of Epidemiology 110:449–461
    [Google Scholar]
  11. Köhler G., Milstein C. 1975; Continuous cultures of fused cells secreting antibody of predefined specificity. Nature, London 256:495–497
    [Google Scholar]
  12. Kung H. C., Jen K. F., Yuan W. C., Tien S. F., Chu C. M. 1978; Influenza in China in 1977: recurrence of influenza A virus sub-type (H1N1). Bulletin of the World Health Organization 56:913–918
    [Google Scholar]
  13. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  14. Laver W. G., Downie J. C. 1976; Influenza virus recombination. I. Matrix protein markers and segregation during mixed infections. Virology 70:105–117
    [Google Scholar]
  15. Lu-BaoLan Webster R. G., Brown L. F., Nerome K. 1983; Analysis of antigenic drift in influenza B viruses: do pooled monoclonal antibodies have any utility as standard reference reagents?. Bulletin of the World Health Organization (in press)
    [Google Scholar]
  16. Lubeck M. D., Schulman J. L., Palese P. 1980; Antigenic variants of influenza viruses: marked differences in the frequencies of variants selected with different monoclonal antibodies. Virology 102:458–462
    [Google Scholar]
  17. Nakajima S., Cox N. J., Kendal A. P. 1981; Antigenic and genome analysis of influenza A (H1N1) viruses from different regions of the world, February 1978–March 1980. Infection and Immunity 32:287–294
    [Google Scholar]
  18. Natali A., Oxford J. S., Schild G. C. 1981; Frequency of naturally occurring antibody to influenza virus antigenic variants selected in vitro with monoclonal antibody. Journal of Hygiene 87:185–190
    [Google Scholar]
  19. Oxford J. S., Corcoran T., Schild G. C. 1980; Naturally occurring temperature-sensitive influenza A viruses of the H1N1 and H3N2 subtypes. Journal of General Virology 48:383–389
    [Google Scholar]
  20. Oxford J. S., Corcoran T., Schild G. C. 1981; Intratypic electrophoretic variation of structural and non-structural polypeptides of human influenza A viruses. Journal of General Virology 56:431–436
    [Google Scholar]
  21. Palese P., Schulman J. L. 1976; Differences in RNA patterns of influenza A viruses. Journal of Virology 17:876–884
    [Google Scholar]
  22. Pereira M. S., Chakraverty P. 1982; Influenza in the United Kingdom 1977–81. Journal of Hygiene 88:501–512
    [Google Scholar]
  23. Racaniello V. R., Palese P. 1979; Influenza B virus genome: assignment of viral polypeptides to RNA segments. Journal of Virology 29:361–373
    [Google Scholar]
  24. Ritchey M. B., Palese P., Schulman J. L. 1977; Differences in protein patterns of influenza A viruses. Virology 76:122–128
    [Google Scholar]
  25. Schild G. C., Dowdle W. R. 1975; Influenza virus characterisation and diagnostic serology. In The Influenza Viruses and Influenza pp 135–372 Edited by Kilboume E. D. New York: Academic Press;
    [Google Scholar]
  26. Schild G. C., Oxford J. S., De jong J. C., Webster R. G. 1983; Evidence for host cell selection of influenza virus antigenic variants. Nature, London 303:706–709
    [Google Scholar]
  27. Six H. R., Glezen W. P., Kasel J. A., Couch R. B., Griffis C., WEBSTER R. G. 1981; Heterogeneity of influenza viruses isolated from the Houston community during defined epidemic periods. In Genetic Variation among Influenza Viruses pp 505–514 Edited by Nayak D. P. New York: Academic Press;
    [Google Scholar]
  28. Sriram G., Bean W. J., Hinshaw V. S., Webster R. G. 1980; Genetic diversity among avian influenza viruses. Virology 105:592–599
    [Google Scholar]
  29. Ueda M., Tobita K., Sugiura A., Enomoto C. 1978; Identification of hemagglutinin of neuraminidase genes of influenza B virus. Journal of Virology 25:685–686
    [Google Scholar]
  30. Webster R. G., Berton M. T. 1981; Analysis of antigenic drift in the haemagglutinin molecule of influenza B virus with monoclonal antibodies. Journal of General Virology 54:243–251
    [Google Scholar]
  31. Webster R. G., Kendal A. P., Gerhard W. 1979; Analysis of antigenic drift in recently isolated influenza (H1N1) viruses using monoclonal antibody preparations. Virology 96:258–264
    [Google Scholar]
  32. Yewdell J. W., Webster R. G., Gerhard W. U. 1979; Antigenic variation in three distinct determinants of an influenza A haemagglutinin molecule. Nature, London 279:246–248
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-11-2367
Loading
/content/journal/jgv/10.1099/0022-1317-64-11-2367
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error