1887

Abstract

SUMMARY

Cloned cells of the rat kidney fibroblast line designated NRK-49F, which requires epidermal growth factor (EGF), fibronectin, insulin, and retinoic acid for rapid multiplication in serum-free culture, were transformed by polyoma virus. Cells from two independent transformation events were isolated and cloned, as were cells from two corresponding control untransformed cultures not treated with virus. Tests in serum-free culture showed that the two transformed subclones required EGF and fibronectin but not insulin or retinoic acid for rapid multiplication, whereas the two control subclones retained the requirements for all four factors. Although EGF at 10 to 50 ng/ml stimulated the multiplication of all four subclones, EGF at 500 ng/ml strongly inhibited multiplication of the two transformed subclones but not the two control subclones.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-10-2311
1983-10-01
2022-01-24
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/10/JV0640102311.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-10-2311&mimeType=html&fmt=ahah

References

  1. Barnes D., Sato G. 1980; Serum-free culture: a unifying approach. Cell 22:649–655
    [Google Scholar]
  2. Bradshaw G. L., Dubes G. R. 1983a; Supplementary factors required for serum free culture of rat kidney cells of line NRK-49F. In vitro (in press)
    [Google Scholar]
  3. Bradshaw G. L., Dubes G. R. 1983b; Polyoma virus transformation of rat kidney fibroblasts results in loss of requirement for insulin and retinoic acid. Proceedings of the American Association for Cancer Research (in press)
    [Google Scholar]
  4. Cherington P. V., Smith B. L., Pardee A. B. 1979; Loss of epidermal growth factor requirement and malignant transformation. Proceedings of the National Academy of Sciences, U. S. A 76:3937–3941
    [Google Scholar]
  5. Cooper J. E. K. 1973; Microtest plating. In Tissue Culture Methods and Applications pp 266–268 Edited by Druse P. F. Jr, Patterson M. K. Jr New York: Academic Press;
    [Google Scholar]
  6. Delarco J. E., Todaro G. J. 1978; Epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (EGF) receptors and the effect of mouse sarcoma virus transformation. Journal of Cellular Physiology 94:335–342
    [Google Scholar]
  7. Jainchill J. L., Todaro G. J. 1970; Stimulation of cell growth in vitro by serum with and without growth factor. Experimental Cell Research 59:137–146
    [Google Scholar]
  8. McClure D. B., Hightower M. J., Topp W. C. 1982; Effect of SV40 transformation on the growth-factor requirements of the rat embryo cell line REF52 in serum-free medium. Cold Spring Harbor Conference on Cell Proliferation 9:345–364
    [Google Scholar]
  9. Rockwell G. A., Sato G. H., Mcclure D. B. 1980; The growth requirements of SV40 virus transformed Balb/c- 3T3 cells in serum-free monolayer culture. Journal of Cellular Physiology 103:323–331
    [Google Scholar]
  10. Rudland P. S., Eckhart W., Gospodarowicz D., Seifert W. 1974; Transformation mutants are not susceptible to growth activation by fibroblast growth factor at permissive temperatures. Nature, London 250:337–339
    [Google Scholar]
  11. Scher C. D., Pledger W. J., Martin P., Antoniades H., Stiles C. D. 1978; Transforming viruses directly reduce the cellular growth requirements for a platelet-derived growth factor. Journal of Cellular Physiology 97:371–380
    [Google Scholar]
  12. Shin S. I., Freedman V. H., Risser R., Pollack R. 1975; Tumorigenicity of virus-transformed cells in nude mice is correlated specifically with anchorage-independent growth in vitro. Proceedings of the National Academy of Sciences, U. S. A 72:4435–4439
    [Google Scholar]
  13. Snedecor G. W., Cochran W. G. 1967; Analysis of variance. In Statistical Methods 6th edn pp 258–264 Ames: Iowa State University Press;
    [Google Scholar]
  14. Soeda E., Kimura G., Miura K. 1978; Similarity of nucleotide sequences around the origin of DNA replication in mouse polyoma virus and simian virus 40. Proceedings of the National Academy of Sciences, U. S. A 75:162–66
    [Google Scholar]
  15. Stiles C. D., Capone G. T., Scher C. D., Antoniades H. N., van Wyk J. J., Pledger W. J. 1979; Dual control of cell growth by somatomedins and platelet-derived growth factor. Proceedings of the National Academy of Sciences, U. S. A 76:1279–1283
    [Google Scholar]
  16. Taub M., U B., Chuman L., Rindler M. J., Saier H. Jr, Sato G. 1981; Alterations in growth requirements of kidney epithelial cells in defined medium associated with malignant transformation. Journal of Supramolecular Structure and Cellular Biochemistry 15:63–72
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-10-2311
Loading
/content/journal/jgv/10.1099/0022-1317-64-10-2311
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error