1887

Abstract

SUMMARY

The envelope of the bunyavirus La Crosse contains two glycoproteins, G1 (120000 mol. wt.) and G2 (38000 mol. wt.). When incubated with trypsin or plasmin, the G1 glycoprotein of virus grown in cell culture was cleaved, leaving two different sized polypeptides in the envelope (67000 and 95000 mol. wt.). Chymotrypsin cleaved G1 leaving polypeptides of 70000 and 100000 mol. wt. G2, however, was not altered by these enzymes. When used in antibody neutralization studies, these proteolytically modified viruses were neutralized approximately 1 to 2 log units in 60 min while control virus was neutralized by over 4 log units in 20 min. Because antibody to G1, but not G2, was involved in La Crosse virus neutralization, cleavage of G1 appeared to be directly responsible for these altered kinetics of neutralization. Antibody did bind to the polypeptides remaining associated with the envelope resulting in infectious virus-antibody complexes. This indicated that a critical site in terms of antibody neutralization was removed from G1 by proteolytic enzymes.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-10-2147
1983-10-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/10/JV0640102147.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-10-2147&mimeType=html&fmt=ahah

References

  1. Bonner W. M., Laskey R. A. 1974; A film detection method for tritium-labeled proteins and nucleic acids in polyacrylamide gels. European Journal of Biochemistry 46:83–88
    [Google Scholar]
  2. Chanas A. C., Gould E. A., Clegg J. C. S., Varma M. G. R. 1982; Monoclonal antibodies to Sindbis virus glycoprotein E1 can neutralize, enhance infectivity, and independently inhibit haemagglutination or haemolysis. Journal of General Virology 58:37–46
    [Google Scholar]
  3. Clarke D. H., Casals J. 1958; Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. American Journal of Tropical Medicine and Hygiene 7:561–573
    [Google Scholar]
  4. Clewley J., Gentsch J., Bishop D. H. L. 1977; Three unique viral RNA species of snowshoe hare and La Crosse bunyaviruses. Journal of Virology 22:459–468
    [Google Scholar]
  5. Dalrymple J. M., Schlesinger S., Russell P. K. 1976; Antigenic characterization of two Sindbis envelope glycoproteins separated by isoelectric focusing. Virology 69:93–103
    [Google Scholar]
  6. Fries E., Helenius A. 1979; Binding of Semliki Forest virus and its spike glycoproteins to cells. European Journal of Biochemistry 97:213–220
    [Google Scholar]
  7. Gentsch J. R., Rozhon E. J., Klimas R. A., El Said L. H., Shope R. E., Bishop D. H. L. 1980; Evidence from recombinant bunyavirus studies that the M RNA gene products elicit neutralizing antibodies. Virology 102:190–204
    [Google Scholar]
  8. Habel K. (editor) 1969; Virus neutralization test. In Fundamental Techniques in Virology pp 288–296 New York: Academic Press;
    [Google Scholar]
  9. Homma M., Ohuchi M. 1973; Trypsin action on the growth of Sendai virus in tissue culture cells. Ⅲ. Structural differences of Sendai viruses grown in eggs and tissue culture cells. Journal of Virology 12:1457–1465
    [Google Scholar]
  10. Hummel B. C. W. 1959; A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin. Canadian Journal of Biochemistry and Physiology 37:1393–1399
    [Google Scholar]
  11. Ito H., Iwasa S. 1980; Simple serological diagnosis of arboviruses: a simplified diluent system for Japanese encephalitis virus hemagglutination-inhibition tests using formalinized chick erythrocytes. Journal of Virological Methods 1:299–302
    [Google Scholar]
  12. Kelley J. M., Emerson S. U., Wagner R. R. 1972; The glycoprotein of vesicular stomatitis virus is the antigen that gives rise to and reacts with neutralizing antibody. Journal of Virology 10:1231–1235
    [Google Scholar]
  13. Kingsford L., Emerson S. U., Kelley J. M. 1980; Separation of cyanogen bromide-cleaved peptides of the vesicular stomatitis virus glycoprotein and analysis of their carbohydrate content. Journal of Virology 36:309–316
    [Google Scholar]
  14. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  15. Maizel J. V. Jr 1971; Polyacrylamide gel electrophoresis of viral proteins. Methods in Virology 5:179–246
    [Google Scholar]
  16. Massey R. J., Schochetman G. 1981; Topographical analysis of viral epitopes using monoclonal antibodies. Mechanism of virus neutralization. Virology 115:20–32
    [Google Scholar]
  17. Montelaro R. C., Rueckert R. R. 1975; Radiolabeling of proteins and viruses in vitro by acetylation with radioactive acetic anhydride. Journal of Biological Chemistry 250:1413–1421
    [Google Scholar]
  18. Obijeski J. F., Bishop D. H. L., Murphy F. A., Palmer E. L. 1976; Structural proteins of La Crosse virus. Journal of Virology 19:985–997
    [Google Scholar]
  19. O’Farrell P. Z., Gold L. M., Huang W. M. 1973; The identification of prereplicative bacteriophage T4 proteins. Journal of Biological Chemistry 248:5499–5501
    [Google Scholar]
  20. Porterfield J. S., Casals J., Chumakov M. P., Gaidamovich S. Ya., Hannoun C., Holmes I. H., Horzinek M. C., Mussgay M., Oker-Blom N., Russell P. K. 1975/1976; Bunyaviruses and Bunyaviridae. Intervirology 6:13–24
    [Google Scholar]
  21. Qureshi A. A., Trent D. W. 1973; Group B arbovirus structural and nonstructural antigens. I. Serological identification of Saint Louis encephalitis virus soluble antigens. Infection and Immunity 7:242–248
    [Google Scholar]
  22. Sheffield J. B., Zacharchuk C. M., Taraschi N., Daly T. M. 1976; Effect of trypsin on mouse mammary tumor virus. Journal of Virology 19:255–266
    [Google Scholar]
  23. Skehel J. J., Waterfield M. D. 1975; Studies on the primary structure of the influenza virus hemagglutinin. Proceedings of the National Academy of Sciences, U. S. A 72:93–97
    [Google Scholar]
  24. Ter Meulen V., Lӧffler S., Carter M. J., Stephenson J. R. 1981; Antigenic characterization of measles and SSPE virus haemagglutinin by monoclonal antibodies. Journal of General Virology 57:357–364
    [Google Scholar]
  25. Vorndam A. V., Trent D. W. 1979; Oligosaccharides of the California encephalitis viruses. Virology 95:1–7
    [Google Scholar]
  26. Webster R. G., Hinshaw V. S., Laver W. G. 1982; Selection and analysis of antigenic variants of the neuraminidase on N2 influenza vuses with monoclonal antibodies. Virology 117:93–104
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-10-2147
Loading
/content/journal/jgv/10.1099/0022-1317-64-10-2147
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error