1887

Abstract

SUMMARY

Two strains of human parainfluenza virus 2 (HPV2), P 1972/6 and P 1980, grow to high titre in MEK cells, and their structural proteins and virus-induced protein synthesis have been characterized by gel electrophoresis and immunoprecipitation. Purified viruses contain seven polypeptides, including cellular actin: L (175K mol. wt.), HN (72K to 74K), NP (66K to 67K), F (52K to 58K), P (49K), A (44.5K) and M (39K). Virus-induced polypeptide synthesis was first detected at 8 h post-infection with the appearance of NP; other major structural proteins were detected from 10 to 12 h after infection and onwards. The synthesis of both the structural glycoproteins was demonstrated, although proteolytic processing could not be detected. Reproducible differences in the gel migration of the HN, F and NP polypeptides were found in whole virus, in infected cells and cells subjected to immunoprecipitation. These differences may reflect genetic diversity within HPV2 and provide a means of probing the molecular epidemiology of these viruses.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-10-2117
1983-10-01
2024-03-29
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/10/JV0640102117.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-10-2117&mimeType=html&fmt=ahah

References

  1. Bernstein J. M., Hruska J. F. 1981; Respiratory syncytial virus proteins: identification by immunoprecipitation. Journal of Virology 38:278–285
    [Google Scholar]
  2. Buetti E., Choppin P. W. 1977; The transcriptase complex of paramyxovirus SV5. Virology 82:493–508
    [Google Scholar]
  3. Caliguiri L. A., Klenk H.-D., Choppin P. W. 1969; The proteins of the parainfluenza virus SV5. 1. Separation of virion polypeptides by polyacrylamide gel electrophoresis. Virology 39:460–466
    [Google Scholar]
  4. Chamberlain J. P. 1979; Fluorographic detection of radioactivity in polyacrylamide gels with a water-soluble fluor, sodium salicylate. Analytical Biochemistry 98:132–135
    [Google Scholar]
  5. Chanock R. M. 1956; Association of a new type of cytopathogenic myxovirus with infantile croup. Journal of Experimental Medicine 104:555–576
    [Google Scholar]
  6. Chanock R. M., Parrott R. H., Cook M. K., Andrews B. E., Bell J. A., Reichelderfer T., Kapikian A. Z., Mastrota F. M., Huebner R. J. 1958; Newly recognised myxoviruses from children with respiratory disease. New England Journal of Medicine 258:207–213
    [Google Scholar]
  7. Etkind P. R., Cross R. K., Lamb R. A., Merz D. C., Choppin P. W. 1980; In vitro synthesis of structural and nonstructural proteins of Sendai and SV5 viruses. Virology 100:22–33
    [Google Scholar]
  8. Giuffre R. M., Tovell D. R., Kay C. M., Tyrrell D. L. 1982; Evidence of an interaction between the membrane protein of a paramyxovirus and actin. Journal of Virology 42:963–968
    [Google Scholar]
  9. Glezen W. P., Loda F. A., Denny F. W. 1976; The parainfluenza virus. In Viral Infections of Humans: Epidemiology and Control pp 337–349 Edited by Evans A. S. New York: Plenum Press;
    [Google Scholar]
  10. Goswami K. K. A., Russell W. C. 1982; A comparison of paramyxoviruses by immunoprecipitation. Journal of General Virology 60:177–183
    [Google Scholar]
  11. Guskey L. E., Bergtrom G. 1981; High yield growth and purification of human parainfluenza type 3 virus and initial analysis of viral structural proteins. Journal of General Virology 54:115–123
    [Google Scholar]
  12. Herrler G., Compans P. W. 1982; Synthesis of mumps virus polypeptides in infected Vero cells. Virology 119:430–438
    [Google Scholar]
  13. Hightower L. E., Bratt M. A. 1974; Protein synthesis in Newcastle disease virus (NDV) infected chick embryo cells. Journal of Virology 13:788–800
    [Google Scholar]
  14. Hightower L. E., Morrison T. G., Bratt M. A. 1975; Relationships among the polypeptides of Newcastle disease virus. Journal of Virology 16:1599–1607
    [Google Scholar]
  15. Johnson K. M., Chanock R. M., Cook M. K., Huebner R. J. 1960; Studies of a new human hemadsorption virus. I. Isolation, properties and characterization. American Journal of Hygiene 71:81–92
    [Google Scholar]
  16. Kennett M. L., Ellis A. W., Lewis F. A., Gust I. D. 1972; An epidemic associated with echovirus type 18. Journal of Hygiene 70:325–334
    [Google Scholar]
  17. Kessler S. W. 1975; Rapid isolation of antigens from cells with staphylococcal protein A-antibody adsorbent: parameters of the interaction of antibody-antigen complexes with protein A. Journal of Immunology 115:1617–1624
    [Google Scholar]
  18. Kingsbury D. W. 1977; Paramyxoviruses. In The Molecular Biology of Animal Viruses pp 349–382 Edited by Nayak D. P. New York: Marcel Dekker;
    [Google Scholar]
  19. Lamb R. A., Mahy B. W. J., Choppin P. W. 1976; The synthesis of Sendai virus polypeptides in infected cells. Virology 69:116–131
    [Google Scholar]
  20. Lamb R. A., Peluso R. W., Choppin P. W. 1978; SV5 and Sendai virus polypeptides: aspects of composition, synthesis and phosphorylation. In Negative Strand Viruses and the Host Cell pp 195–203 Edited by Mahy B. W. J., Barry R. D. London: Academic Press;
    [Google Scholar]
  21. Mountcastle W. E., Compans R. W., Lackland H., Choppin P. W. 1974; Proteolytic cleavage of subunits of the nucleocapsid of the paramyxovirus SV5. Journal of Virology 14:1253–1261
    [Google Scholar]
  22. Peluso R. W., Lamb R. A., Choppin P. W. 1977; Polypeptide synthesis in simian virus 5-infected cells. Journal of Virology 23:177–187
    [Google Scholar]
  23. Saborio J. L., Pong S.-S., Koch G. 1974; Selective and reversible inhibition of protein synthesis in mammalian cells. Journal of Molecular Biology 85:195–211
    [Google Scholar]
  24. Scheid A., Choppin P. W. 1977; Two disulfide-linked polypeptide chains constitute the active F protein of paramyxoviruses. Virology 80:54–66
    [Google Scholar]
  25. Shimokata K., Ito Y., Nishiyama Y., Kimura Y. 1980; Influence of trypsin on the infectivity and biological properties of parainfluenza type 2 (croup-associated) virus in Vero cells. Journal of General Virology 48:407–410
    [Google Scholar]
  26. Stephenson J., Ter Meulen V. 1979; Antigenic relationships between measles and canine distemper viruses: comparison of immune response in animals and humans to individual virus-specific polypeptides. Proceedings of the National Academy of Sciences, U. S. A 76:6601–6605
    [Google Scholar]
  27. Welliver R., Wong D. T., Choi T.-S., Ogra P. L. 1982; Natural history of parainfluenza virus infection in childhood. Journal of Pediatrics 101:180–187
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-10-2117
Loading
/content/journal/jgv/10.1099/0022-1317-64-10-2117
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error