1887

Abstract

SUMMARY

We have recently found that, , the murine leukaemia virus (MuLV)-associated protein kinase activity predominantly phosphorylates Pr65, a virus protein present in relatively small amounts in partially purified virus preparations. Other virus proteins, such as p10, Pr27 and Pr40, are also phosphorylated , but to a lesser degree. Furthermore, when immature core subparticles which are enriched in Pr65 are prepared from virions by Sepharose 6B exclusion column chromatography, about 50% of the kinase activity (as assayed with the exogenous substrate phosvitin) remains associated with the cores. We report here that this core-associated activity is distinct from Pr65 since it can be separated from Pr65 by chromatography on denatured DNA-cellulose columns followed by centrifugation of the 0.2 -NaCl-eluted fraction. Under these conditions, Pr65 is pelleted while the kinase activity, which can phosphorylate both endogenous (MuLV Pr65 and p10) as well as exogenous (phosvitin) substrates, remains in the supernatant. Interestingly, when the amount of Pr65 is reduced, as in such preparations, p10 then becomes more heavily phosphorylated.

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1983-01-01
2024-04-25
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References

  1. Bunte T., Owada M. K., Donner P., Boscheck C. B., Moelling K. 1981; Association of the transformation-specific protein pp60src with the membrane of an avian sarcoma virus. Journal of Virology 38:1034–1042
    [Google Scholar]
  2. Erikson R. L., Collett M. S., Erikson E., Purchio A. F. 1979; Evidence that the avian sarcoma virus transforming gene product is a cyclic AMP-independent protein kinase. Proceedings of the National Academy of Sciences of the United States of America 76:6260–6264
    [Google Scholar]
  3. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  4. Long C. W., Henderson L. E., Oroszlan S. 1980; Isolation and characterization of low molecular weight DNA-binding proteins from retroviruses. Virology 104:491–496
    [Google Scholar]
  5. Lu A. H., Soong M. M., Wong P. K. Y. 1979; Maturation of Moloney murine leukemia virus. Virology 93:269–274
    [Google Scholar]
  6. Naso R. B., Karshin W. L., Wu Y. H., Arlinghaus R. B. 1979; Characterization of 40, 000- and 25, 000-dalton intermediate precursors to Rauscher murine leukemia virus gag gene products. Journal of Virology 32:187–198
    [Google Scholar]
  7. Oroszlan S., Gilden R. B. 1980; Primary structure analysis of retrovirus protein. In Molecular Biology of RNA Tumor Viruses pp 299–344 Edited by Stephenson J. E. New York: Academic Press;
    [Google Scholar]
  8. Oroszlan S., Long C. W., Gilden R. B. 1976; Isolation of a murine type-C virus p30 precursor protein by DNA-cellulose chromatography. Virology 72:523–526
    [Google Scholar]
  9. Strand M., August J. T. 1971; Protein kinase and phosphate acceptor proteins in Rauscher murine leukemia virus. Nature New Biology 233:137–140
    [Google Scholar]
  10. Witte O. N., Baltimore D. 1978; Relationship of retrovirus polyprotein cleavages to virion maturation studied with temperature-sensitive murine leukemia virus mutants. Journal of Virology 26:750–761
    [Google Scholar]
  11. Yoshinaka Y., Luftig R. B. 1977; Properties of a P70 proteolytic factor of murine leukemia viruses. Cell 12:709–719
    [Google Scholar]
  12. Yoshinaka Y., Luftig R. B. 1978; Morphological conversion of immature Rauscher leukaemia virus cores to a mature form after addition of the P65–70 (gag gene product) proteolytic factor. Journal of General Virology 40:151–160
    [Google Scholar]
  13. Yoshinaka Y., Luftig R. B. 1980; Physicochemical characterization and specificity of the murine leukaemia virus Pr65gag proteolytic factor. Journal of General Virology 48:329–340
    [Google Scholar]
  14. Yoshinaka Y., Luftig R. B. 1982; In vitro phosphorylation of murine leukemia virus proteins: specific phosphorylation of Pr65gag, the precursor to the internal core antigens. Virology 116:181–195
    [Google Scholar]
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