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Rabies virus may be cultivated in cell culture systems that either enhance the virulence of progeny virus (productive infection of neuroblastoma cells) or lead to attenuation (maintenance in chronically infected cell systems). Our studies using 19 monoclonal antibodies that react with glycoprotein and 19 monoclonal antibodies that react with nucleoprotein have shown that these laboratory-induced changes in the biological properties of rabies virus are accompanied by changes in their antigenic phenotype.
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