Effect of Multiplicity of Infection on Transcription in Cells Infected by Bacteriophage Lambda Free

Abstract

SUMMARY

The effect of multiplicity of infection (m.o.i.) on transcription was studied by infecting with bacteriophage I47, I47293 and I857273. DNA-RNA hybridization with γI47 -strand DNA, φ80-strand DNA, and 80 -strand DNA were used to measure mRNA transcription from the -strand, the -strand of the early region and the late -b2 region of bacteriophage respectively. In I47 -infected cells, transcription from the -strand, -strand of the early region and the late -b2 region all decreased with increasing m.o.i. The response in the region was less marked than in other regions, but the pattern looked similar to that described above. When phage DNA replication was permitted, as in the case of I47, the response was similar to that observed in I47 -infected cells, but the level of transcription was increased two- or three-fold. In I857 -infected cells, the leftward transcription and the rightward transcription from the early region and the late -b2 region all increased with increasing m.o.i., but the extent of change was less drastic than with . This result demonstrated clearly that the decreased in transcription from various regions at increasing m.o.i. of was due to the inhibitory action of the gene product. The results obtained with strongly support the view that gene dosage is a significant controlling factor for the extent of gene expression.

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1983-01-01
2024-03-28
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