1887

Abstract

SUMMARY

The effect of multiplicity of infection (m.o.i.) on transcription was studied by infecting with bacteriophage I47, I47293 and I857273. DNA-RNA hybridization with γI47 -strand DNA, φ80-strand DNA, and 80 -strand DNA were used to measure mRNA transcription from the -strand, the -strand of the early region and the late -b2 region of bacteriophage respectively. In I47 -infected cells, transcription from the -strand, -strand of the early region and the late -b2 region all decreased with increasing m.o.i. The response in the region was less marked than in other regions, but the pattern looked similar to that described above. When phage DNA replication was permitted, as in the case of I47, the response was similar to that observed in I47 -infected cells, but the level of transcription was increased two- or three-fold. In I857 -infected cells, the leftward transcription and the rightward transcription from the early region and the late -b2 region all increased with increasing m.o.i., but the extent of change was less drastic than with . This result demonstrated clearly that the decreased in transcription from various regions at increasing m.o.i. of was due to the inhibitory action of the gene product. The results obtained with strongly support the view that gene dosage is a significant controlling factor for the extent of gene expression.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-1-183
1983-01-01
2022-08-10
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/1/JV0640010183.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-1-183&mimeType=html&fmt=ahah

References

  1. Campbell A. 1961; Sensitive mutants of bacteriophage λ . Virology 14:22–32
    [Google Scholar]
  2. Cohen S. N., Chang A. C. Y. 1970; Genetic expression in bacteriophage λ. Ⅲ. Inhibition of Escherichia coli nucleic acid and protein synthesis during λ development. Journal of Molecular Biology 49:557–575
    [Google Scholar]
  3. Court D., Green L., Echols H. 1975; sPositive and negative regulation by the cⅡ and cⅢ gene products of bacteriophage λ . Virology 63:484–491
    [Google Scholar]
  4. Dove W. F. 1966; Action of the lambda chromosome. I. Control of function late in bacteriophage development. Journal of Molecular Biology 19:187–201
    [Google Scholar]
  5. Edstrom J.-E., Lambert B. 1975; Gene and information diversity in eukaryotes. Progress in Biophysics and Molecular Biology 30:57–82
    [Google Scholar]
  6. Eisen H., Brachet P., Pereira Da Silva L., Jacob F. 1970; Regulation of repressor expression in λ . Proceedings of the National Academy of Sciences of the United States of America 66:855–862
    [Google Scholar]
  7. Franklin N. C., Dove W. F., Yanofsky C. 1965; The linear insertion of a prophage into the chromosome of E.coli shown by deletion mapping. Biochemical and Biophysical Research Communications 18:910–923
    [Google Scholar]
  8. Herskowitz I., Hagen D. 1980; The lysis-lysogeny decision of phage λ: explicit programming and responsiveness. Annual Review of Genetics 14:399–445
    [Google Scholar]
  9. Kaiser A. D. 1957; Mutations in a temperate bacteriophage affecting its ability to lysogenize Escherichia coli . Virology 3:42–61
    [Google Scholar]
  10. Kourilsky P., Bourguignon M. F., Gros F. 1971; Kinetics of viral transcription after induction of prophage. In The Bacteriophage Lambda pp 647–666 Edited by Hershey A. D. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  11. Luk K. C., Mark K. K. 1980; The phage promoter responsible for the expression of the inserted β-galactosidase gene in bacteriophage λpl ac5. Molecular and General Genetics 178:555–560
    [Google Scholar]
  12. Luk K. C., Mark K. K. 1982; Gene dosage as a regulatory factor for gene expression. I. In λplac 5-infected cells. Journal of General Virology 58:297–304
    [Google Scholar]
  13. McMacken R., Mantei N., Butler B., Joyner A., Echols H. 1970; Effect of mutations on the cⅡ and cⅢ genes of bacteriophage λ on macromolecular synthesis in infected cells. Journal of Molecular Biology 49:639–655
    [Google Scholar]
  14. Mark K. K. 1970; The relationship between the synthesis of DNA and the synthesis of phage lysozyme in Escherichia coli infected by bacteriophage T4. Virology 42:20–27
    [Google Scholar]
  15. Mark K. K. 1973; Effect of the N-bypass mutations nin and byp on the rightward transcription in coliphage lambda. Molecular and General Genetics 124:291–304
    [Google Scholar]
  16. Meyer B. J., Manner R., Ptashne M. 1980; Gene regulation at the right operator (OR) of bacteriophage A. II. O R1, O r2, and O R3: their role in mediating the effect of repressor and cro . Journal of Molecular Biology 139:163–194
    [Google Scholar]
  17. Nijkamp H. J. J., Bøvre K., Szybalski W. 1970; Controls of rightward transcription in coliphage λ . Journal of Molecular Biology 54:599–604
    [Google Scholar]
  18. Ogawa T., Tomizawa U. 1968; Replication of bacteriophage DNA. I. Replication of DNA of lambda phage defective in early function. Journal of Molecular Biology 38:217–225
    [Google Scholar]
  19. Ptashne M., Jeffrey A., Johnson A. D., Maurer R., Meyer B. J., Pabo C. O., Roberts T. M., Sauer R. T. 1980; How the λ repressor and cro work. Cell 19:1–11
    [Google Scholar]
  20. Ray P. N., Pearson M. L. 1976; Synthesis of morphogenetic proteins by mutants of bacteriophage lambda carrying tandem genetic duplications. Virology 73:381–388
    [Google Scholar]
  21. Roberts J. W. 1975; Transcription termination and late control in phage lambda. Proceedings of the National Academy of Sciences of the United States of America 72:3300–3304
    [Google Scholar]
  22. Signer E. R. 1964; Recombination between coliphages λ and ϕ 80. Virology 22:650–651
    [Google Scholar]
  23. Sklar J., Yot P., Weissman S. 1975; Determination of genes, restriction sites, and DNA sequences surrounding the 6S RNA template of bacteriophage lambda. Proceedings of the National Academy of Sciences of the United States of America 72:1817–1821
    [Google Scholar]
  24. Szpirer J., Thomas R., Radding C. M. 1969; Hybrids of bacteriophage λ and ϕ80. A study of nonvegetative functions. Virology 37:585–596
    [Google Scholar]
  25. Takeda Y. 1975; Regulation of early protein synthesis in bacteriophage λ . Virology 66:629–631
    [Google Scholar]
  26. Takeda Y., Folkmanis A., Echols H. 1977; Cro regulatory protein specified by bacteriophage A. Journal of Biological Chemistry 252:6177–6183
    [Google Scholar]
  27. Tartof K. D. 1975; Redundant genes. Annual Review of Genetics 9:355–385
    [Google Scholar]
  28. Tsui L., Mark K. K. 1976; The depression of endolysin synthesis in bacteria infected with high multiplicities of phage A. Molecular and General Genetics 143:269–278
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-1-183
Loading
/content/journal/jgv/10.1099/0022-1317-64-1-183
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error