Specificity of IgM Antibodies in Acute Human Coxsackievirus B Infections, Analysed by Indirect Solid Phase Enzyme Immunoassay and Immunoblot Technique Free

Abstract

SUMMARY

The specificity of the IgM response in acute human coxsackievirus B infections was examined by indirect solid phase enzyme immunoassay and immunoblot techniques. IgM antibodies detected by ELISA were either strictly type-specific, type-predominant or group-reactive to coxsackieviruses B-1 to B-5. Homotypic and type-dominant responses were clearly correlated with the serotype isolated from the patient. Analysis of ELISA antigens by SDS-gel electrophoresis, protein transfer and subsequent immunodetection of individual virus polypeptides, revealed VP1 as the major antigen; it was detected by homotypic as well as heterotypic serum specimens.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-64-1-159
1983-01-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/64/1/JV0640010159.html?itemId=/content/journal/jgv/10.1099/0022-1317-64-1-159&mimeType=html&fmt=ahah

References

  1. Bidwell D. E., Bartlett A., Voller A. 1977; Enzyme immunoassay for viral diseases. Journal of Infectious Diseases 136:274–278
    [Google Scholar]
  2. Burnette W. N. 1981; Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Analytical Biochemistry 112:195–203
    [Google Scholar]
  3. Dörries R., Ter Meulen V. 1980; Detection of enterovirus specific IgG and IgM antibodies in humans by an indirect solid phase radioimmunoassay. Medical Microbiology and Immunology 168:159–171
    [Google Scholar]
  4. El-Hagrassy M. M. O., Banatvala J. E., Coltart D. J. 1980; Coxsackie-B-virus-specific IgM responses in patients with cardiac and other diseases. Lancet 1:1160–1162
    [Google Scholar]
  5. Engvall E., Perlman P. 1972; Enzyme-linked immunosorbent assay, ELISA. III. Quantitation of specific antibodies by enzyme-labelled anti-immunoglobulin in antigen-coated tubes. Journal of Immunology 109:129–135
    [Google Scholar]
  6. Grist N. R., Bell E. J., Assad F. 1978; Enteroviruses in human diseases. Progress in Medical Virology 24:114–157
    [Google Scholar]
  7. Herrmann J. E., Hendry R. M., Collins M. F. 1979; Factors involved in enzyme-linked immunoassay of viruses and evaluation of the method for identification of enteroviruses. Journal of Clinical Microbiology 10:210–217
    [Google Scholar]
  8. Katze M. G., Crowell R. L. 1980; Immunological studies of the group B coxsackieviruses by the sandwich enzyme-linked immunosorbent assay (ELISA) and immunoprecipitation. Journal of General Virology 50:357–367
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  10. Philips C. A. 1976; Enteroviruses. In Manual of Clinical Microbiology pp 723–727 Edited by Lennette E. H., Spaulding E. H., Traunt J. P. Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  11. Rueckert R. R. 1976; On the structure and morphogenesis of picomaviruses. In Comprehensive Virology Vol 6: pp 131–200 Edited by Fraenkel-Conrat H., Wagner R. R. New York & London: Plenum Press;
    [Google Scholar]
  12. Schmidt N. J., Lennette E. H., Dennis J. 1968; Characterization of antibodies produced in natural and experimental coxsackievirus infections. Journal of Immunology 100:99–106
    [Google Scholar]
  13. Voller A., Bid Well D., Bartlett A. 1976; Microplate enzyme immunoassays for the immunodiagnosis of virus infections. In Manual of Clinical Immunology pp 506–512 Edited by Rose N. R., Friedman H. Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  14. Ziegler D. W. 1979; Determination of IgM antibodies in diagnostic virology. In Diagnosis of Viral Infections pp 63–71 Edited by Lennette D. A., Specter S., Thompson K. D. Baltimore: University Park Press;
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-64-1-159
Loading
/content/journal/jgv/10.1099/0022-1317-64-1-159
Loading

Data & Media loading...

Most cited Most Cited RSS feed