RT Journal Article SR Electronic(1) A1 Dörries, R. A1 Ter Meulen, V.YR 1983 T1 Specificity of IgM Antibodies in Acute Human Coxsackievirus B Infections, Analysed by Indirect Solid Phase Enzyme Immunoassay and Immunoblot Technique JF Journal of General Virology, VO 64 IS 1 SP 159 OP 167 DO https://doi.org/10.1099/0022-1317-64-1-159 PB Microbiology Society, SN 1465-2099, AB SUMMARY The specificity of the IgM response in acute human coxsackievirus B infections was examined by indirect solid phase enzyme immunoassay and immunoblot techniques. IgM antibodies detected by ELISA were either strictly type-specific, type-predominant or group-reactive to coxsackieviruses B-1 to B-5. Homotypic and type-dominant responses were clearly correlated with the serotype isolated from the patient. Analysis of ELISA antigens by SDS-gel electrophoresis, protein transfer and subsequent immunodetection of individual virus polypeptides, revealed VP1 as the major antigen; it was detected by homotypic as well as heterotypic serum specimens., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-1-159