@article{mbs:/content/journal/jgv/10.1099/0022-1317-64-1-159, author = "Dörries, R. and Ter Meulen, V.", title = "Specificity of IgM Antibodies in Acute Human Coxsackievirus B Infections, Analysed by Indirect Solid Phase Enzyme Immunoassay and Immunoblot Technique", journal= "Journal of General Virology", year = "1983", volume = "64", number = "1", pages = "159-167", doi = "https://doi.org/10.1099/0022-1317-64-1-159", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-64-1-159", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "immunoglobulin M response", keywords = "coxsackievirus B", keywords = "ELISA", keywords = "protein-blot", abstract = "SUMMARY The specificity of the IgM response in acute human coxsackievirus B infections was examined by indirect solid phase enzyme immunoassay and immunoblot techniques. IgM antibodies detected by ELISA were either strictly type-specific, type-predominant or group-reactive to coxsackieviruses B-1 to B-5. Homotypic and type-dominant responses were clearly correlated with the serotype isolated from the patient. Analysis of ELISA antigens by SDS-gel electrophoresis, protein transfer and subsequent immunodetection of individual virus polypeptides, revealed VP1 as the major antigen; it was detected by homotypic as well as heterotypic serum specimens.", }