RNA-dependent RNA Polymerase Activity in Murine Coronavirus-infected Cells Free

Abstract

SUMMARY

The multiplication of murine coronavirus strains A59 or JHM in Sac(-) cells was unaffected by the presence of α-amanitin at concentrations which inhibited the host cell DNA-dependent RNA polymerase activity. In cells infected with the A59 virus strain, actinomycin D-resistant RNA synthesis could readily be detected by pulse-labelling with [H]uridine; this virus-specific RNA synthesis was not induced in the presence of the protein synthesis inhibitor anisomycin. A new RNA-dependent RNA polymerase activity was detected in the large particle fraction of A59 virus-infected cells. Optimal conditions for enzyme activity were established. Maximum activity occurred 5 h after infection, coincident with the peak of virus-specific RNA synthesis detected by pulse-labelling .

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1983-01-01
2024-03-28
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