1887

Abstract

Summary

Prostaglandins of the A series potently inhibited the production of vaccinia virus in mouse L fibroblasts. With the highest non-toxic dose of PGA, 4 µg/ml, the replication of the virus was inhibited by 95.3%. The antiviral activity was dose-dependent and specific for the A series. At the dose used, PGA was not toxic to uninfected cells and did not alter cell metabolism as measured by DNA, RNA and protein synthesis. PGA did not influence the adsorption of the virus by the host cells and the antiviral activity was not dependent on the presence of PGA during the early stages of infection. PGA treatment delayed and partially inhibited virus DNA synthesis and, while it did not produce any change in the pattern of protein synthesis in uninfected cells, it altered both the rate and the pattern of virus protein synthesis. We conclude that PGA selectively inhibits one or more steps involved in the replication of vaccinia virus in mouse L fibroblasts.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-63-2-435
1982-12-01
2022-08-11
Loading full text...

Full text loading...

/deliver/fulltext/jgv/63/2/JV0630020435.html?itemId=/content/journal/jgv/10.1099/0022-1317-63-2-435&mimeType=html&fmt=ahah

References

  1. Esteban M. 1977; Rifampin and vaccinia DNA. Journal of Virology 21:796–801
    [Google Scholar]
  2. Fitzpatrick F. A., Stringfellow D. A. 1980; Virus and interferon effects on cellular prostaglandin biosynthesis. Journal of Immunology 125:431–436
    [Google Scholar]
  3. Goodwin J. S., Webb D. R. 1980; Regulation of the immune response by prostaglandins. Clinical Immunology and Immunopathology 15:106–122
    [Google Scholar]
  4. Hammarstrom S. 1977; Prostaglandin production by normal and transformed 3T3 fibroblasts in cell culture. European Journal of Biochemistry 74:7–12
    [Google Scholar]
  5. Harbour D. A., Blyth W. A., Hill T. J. 1978; Prostaglandins enhance spread of herpes simplex virus in cell cultures. Journal of General Virology 41:87–95
    [Google Scholar]
  6. Jaffe B. M., Santoro M. G. 1977; Prostaglandins and cancer. In The Prostaglandins vol. 3 pp. 329–351 Edited by Ramwell P. W. New York: Plenum Press;
    [Google Scholar]
  7. Jaffe B. M., Behrman J., Parker C. W. 1973; Radioimmunoassay measurement of prostaglandins E, A, and F in human plasma. Journal of Clinical Investigation 52:398–405
    [Google Scholar]
  8. Joklik W. K. 1962; The preparation and characteristics of highly purified radioactively labelled pox virus. Biochimica et biophysica acta 61:290–301
    [Google Scholar]
  9. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  10. Luczak M., Gumulka W., Szwigielski S., Korbecki M. 1975; Inhibition of multiplication of parainfluenza 3 virus in prostaglandin-treated WISH cells. Archives of Virology 49:377–380
    [Google Scholar]
  11. Metz P. H., Esteban M. 1972; Interferon inhibits viral protein synthesis in L cells infected with vaccinia virus. Nature, London 238:385–388
    [Google Scholar]
  12. Pottathil R., Chandrabose K. A., Cuatrecasas P., Lang D. J. 1980; Establishment of the interferon-mediated antiviral state; role of fatty acid cyclooxygenase. Proceedings of the National Academy of Sciences of the United States of America 77:5437–5446
    [Google Scholar]
  13. Ritzi E. M., Stylos W. A. 1976; Prostaglandin production in cultures of BALB/3T3 and SV3T3 mouse fibroblasts. Journal of the National Cancer Institute 56:529–533
    [Google Scholar]
  14. Santoro M. G., Benedetto A., Carruba G., Garaci E., Jaffe B. M. 1980; Prostaglandin A compounds as antiviral agents. Science 209:1032–1034
    [Google Scholar]
  15. Santoro M. G., Carruba G., Garaci E., Jaffe B. M., Benedetto A. 1981; Prostaglandins of the A series inhibit Sendai virus replication in cultured cells. Journal of General Virology 53:75–83
    [Google Scholar]
  16. Stringfellow D. A. 1978; Prostaglandin restoration of the interferon response of hyporeactive animals. Science 201:376–378
    [Google Scholar]
  17. Yaron M., Yaron I., Guarari-Rotman D., Revel M., Lindner H. R., Zor V. 1977; Stimulation of prostaglandin E production in cultured human fibroblasts by poly(I)-poly(C) and human interferon. Nature, London 267:457–459
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-63-2-435
Loading
/content/journal/jgv/10.1099/0022-1317-63-2-435
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error