RT Journal Article SR Electronic(1) A1 Wesley, Ronald D. A1 Pan, I. C.YR 1982 T1 African Swine Fever Virus DNA: Restriction Endonuclease Cleavage Patterns of Wild-type, Vero Cell-adapted and Plaque-purified Virus JF Journal of General Virology, VO 63 IS 2 SP 383 OP 391 DO https://doi.org/10.1099/0022-1317-63-2-383 PB Microbiology Society, SN 1465-2099, AB Summary DNA from African swine fever (ASF) virus was isolated and was characterized by two restriction enzymes, SmaI and EcoRI. Although both enzymes can distinguish Vero cell-adapted ASF isolates by characteristic restriction endonuclease cleavage patterns, all ASF isolates examined exhibited a high degree of similarity, as measured by co-migration of most of the DNA fragments. The molecular weight of ASF DNA, based on size estimates of DNA fragments from cleavage patterns, ranged from 93 × 106 to 100 × 106. Virus genome heterogeneity was observed in uncloned, cell culture-adapted ASF isolates as well as in a plaque-purified virus after serial passage in Vero cells. In contrast to the rather minor differences in restriction pattern among the Vero cell-adapted isolates, a major alteration in restriction endonuclease cleavage sites was observed during adaptation of the wild-type virus to cell culture., UL https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-63-2-383