1887

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Volume 63, Issue 2

Enhancement of Plasminogen Activator Activity by the Culture Medium of Rous Sarcoma Virus-transformed Cells Free

Abstract

Summary

We have tested the effect of the culture medium of chicken embryo fibroblasts (CEF) transformed by Rous sarcoma virus (RSV) on plasminogen activator (PA) activity of normal and RSV-infected cells. The results obtained showed that fibrin digestion by cultures of normal uninfected and RSV-infected cells directly attached to a fibrin substrate was increased when the cells were exposed to the supernatant of an 18 h-culture of CEF transformed by RSV. Addition of cycloheximide to the supernatant completely abolished this effect. Similarly, exposure of both normal and RSV-infected cells to the culture medium of RSV-transformed CEF resulted in a marked increase of the soluble PA activity present in the supernatant. This effect, however, was not abolished by inhibition of protein synthesis. For both the cell-bound and soluble PA activities of normal and RSV-infected cells the stimulatory effect was transient, being maximal between 8 and 12 h of exposure to the transformed cell-conditioned medium and disappearing after 24 h. A possible correlation with transformation-enhancing factor(s), previously reported to be present in the culture medium of transformed cells, is discussed.

  • Received:
  • Accepted:
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Keyword(s): plasminogen activator , Rous sarcoma virus , transformation-enhancing factors and transformed cells
© Society for General Microbiology, 1982
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1982-12-01
2023-06-10
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References

  1. Adelman S. F., Howett M. K., Rapp F. 1980; Quantification of plasminogen activator activity associated with herpesvirus-transformed cells. Journal of General Virology 50:101–110
     [Google Scholar]
  2. Barlati S., Mignatti P. 1980; Transformation-enhancing factors in the plasma of cancer patients. In Control Mechanisms in Animal Cells pp. 269–277 Edited by Jimenez L., De Asua R., Levi-Montalcini R. Shields, Iacobelli S. New York: Raven Press;
     [Google Scholar]
  3. Barlati S., Mignatti P., Brega A., De Petro G., Ascari E. 1978; Utilization of Rous sarcoma virus for the detection of transformation enhancing and inhibiting factors in human plasma. In Avian RNA Tumor Viruses pp. 331–348 Edited by Barlati S., DeGiuli-Morghen C. Padua: Piccin Medical Books;
     [Google Scholar]
  4. Beers W. R., Strickland S., Reich E. 1975; Ovarian plasminogen activator: relationship to ovulation and hormonal regulation. Cell 6:387–394
     [Google Scholar]
  5. Brynes P. J., Schmidt R., Hecher E. 1980; Plasminogen activator induction and platelet aggregation by phorbol and some of its derivatives: correlation with skin irritancy and tumor-promoting activity. Journal of Cancer Research and Clinical Oncology 97:257–266
     [Google Scholar]
  6. Camiolo S. M., Markus G., Evers J. L., Hobika G. H., Depasquale J. L., Beckley S., Grimaldi J. P. 1981; Plasminogen activator content of neoplastic and benign human prostate tissues: fibrin augmentation of an activator activity. International Journal of Cancer 97:191–198
     [Google Scholar]
  7. Christman J. K., Acs G. 1974; Purification and characterisation of a cellular fibrinolytic factor associated with oncogenic transformation: the plasminogen activator from SV40-transformed hamster cells. Biochimica et biophysica acta 340:339–347
     [Google Scholar]
  8. Corasanti J. C., Celik C., Camiolo S. M., Mittelman A., Evers J. L., Barbasch A., Hobika G. H., Markus G. 1980; Plasminogen activator content of human colon tumors and normal mucosae: separation of enzymes and partial purification. Journal of the National Cancer Institute 65:345–351
     [Google Scholar]
  9. De Petro G., Barlati S., Vartio T., Vaheri A. 1981; Transformation-enhancing activity of gelatin-binding fragments of fibronectin. Proceedings of the National Academy of Sciences of the United States of America 78:4965–1969
     [Google Scholar]
  10. Evers J. L., Patel J., Madeja J. M., Schneider S. L., Hobika G. H., Camiolo S. M., Markus G. 1982; Plasminogen activator activity and composition in human breast cancer. Cancer Research 42:219–226
     [Google Scholar]
  11. Fisher A. 1925; Beitrag zur Biologie der Gewebezellen. Eine vergleichend-biologische Studie der normalen und malignen Gewebezellen in vitro . Archiv Entwicklungs 104:210–217
     [Google Scholar]
  12. Goldfarb R. H., Ouigley J. P. 1980; Purification of plasminogen activator from Rous sarcoma virus- transformed chick embryo fibroblasts treated with the tumor promoter phorbol 12-myristate 13-acetate. Biochemistry 19:5463–5471
     [Google Scholar]
  13. Granelli-Piperno A., Vassalli J. D., Reich E. 1977; Secretion of plasminogen activator by human polymorphonuclear leukocytes: modulation by glucocorticoids and other effectors. Journal of Experimental Medicine 146:1693–1706
     [Google Scholar]
  14. Jaken S., Black P. H. 1979; Differences in intracellular distribution of plasminogen activator in growing, confluent and transformed 3T3 cells. Proceedings of the National Academy of Sciences of the United States of America 76:246–250
     [Google Scholar]
  15. Kawai S., Hanafusa H. 1971; The effects of reciprocal changes in temperature on the transformed state of cells infected with a Rous sarcoma virus mutant. Virology 47:426–433
     [Google Scholar]
  16. Kryceve C., Vigier P., Barlati S. 1976; Transformation-enhancing factor(s) produced by virus-transformed and established cells. International Journal of Cancer 17:370–379
     [Google Scholar]
  17. Kryceve-Martinerie C., Biquard J. M., Lawrence D., Vigier P., Barlati S., Mignatti P. 1981; Transformation-enhancing factor(s) released from chicken Rous sarcoma cells: effect on some transformation parameters. Virology 112:436–449
     [Google Scholar]
  18. Lee L. S., Weinstein I. B. 1978; Epidermal growth factor, like phorbol esters, induces plasminogen activator in HeLa cells. Nature, London 274:696–697
     [Google Scholar]
  19. Levin E. J., Loskutoff D. J. 1980; Serum-mediated suppression of cell-associated plasminogen activator activity in cultured endothelial cells. Cell 22:701–707
     [Google Scholar]
  20. Lin H. S., Gordon S. 1979; Secretion of plasminogen activator by bone marrow-derived mononuclear phagocytes and its enhancement by colony-stimulating factor. Journal of Experimental Medicine 150:231–245
     [Google Scholar]
  21. Loskutoff D. J., Edgington T. S. 1977; Synthesis of a fibrinolytic activator and inhibitor by endothelial cells. Proceedings of the National Academy of Sciences of the United Stales of America 74:3903–3907
     [Google Scholar]
  22. Markus G., Takita H., Camiolo S. M., Corasanti J. C., Evers J. L., Hobika G. H. 1980; Content and characterisation of plasminogen activators in human lung tumors and normal lung tissue. Cancer Research 40:841–848
     [Google Scholar]
  23. Mignatti P., Ascari E., Barlati S. 1980; Potential diagnostic and prognostic significance of the transformation-enhancing factor(s) in the plasma cryoprecipitate of tumor patients. International Journal of Cancer 25727–734
     [Google Scholar]
  24. Miskin R., Reich E. 1980; Plasminogen activator: induction of synthesis by DNA damage. Cell 19:217–224
     [Google Scholar]
  25. Miskin R., Easton T. G., Reich E. 1978; Plasminogen activator in chick muscle cells; induction of enzyme by RSV, PMA and retinoic acid. Cell 15:1301–1312
     [Google Scholar]
  26. Moscatelli D., Jaffe E., Rifkin D. B. 1980; Tetradecanoy 1 phorbol acetate stimulates latent collagenase production by cultured human endothelial cells. Cell 20:343–351
     [Google Scholar]
  27. Nagy B., Ban J., Brdar B. 1977; Fibrinolysis associated with human neoplasia: production of plasminogen activator by human tumors. International Journal of Cancer 19:614–620
     [Google Scholar]
  28. O’Donnel-Tormey J., Quigley J. P. 1981; Inhibition of plasminogen activator release from transformed chicken fibroblasts by a protease inhibitor. Cell 27:85–95
     [Google Scholar]
  29. Ossowski L., Quigley J. P., Kellerman G. M., Reich E. 1973; Fibrinolysis associated with oncogenic transformation. Requirement of plasminogen for correlated changes in cellular morphology, colony formation in agar and cell migration. Journal of Experimental Medicine 138:1056–1064
     [Google Scholar]
  30. Ossowski L., Quigley J. P., Reich E. 1974; Fibrinolysis associated with oncogenic transformation. Morphological correlates. Journal of Biological Chemistry 249:4312–4320
     [Google Scholar]
  31. Ossowski L., Biegel D., Reich E. 1979; Mammary plasminogen activator: correlation with involution, hormonal modulation, and comparison between normal and neoplastic tissue. Cell 16:929–940
     [Google Scholar]
  32. Pollack R., Risser R., Conlon S., Rifkin D. B. 1974; Plasminogen activator production accompanies loss of anchorage regulation in transformation of primary rat embryo cells by simian virus 40. Proceedings of the National Academy of Sciences of the United States of America 71:4792–4796
     [Google Scholar]
  33. Quigley J. P. 1976; Association of a protease (plasminogen activator) with a specific membrane fraction isolated from transformed cells. Journal of Cell Biology 71:472–486
     [Google Scholar]
  34. Quigley J. P. 1979; Phorbol ester-induced morphological changes in transformed chick fibroblasts: evidence for direct catalytic involvement of plasminogen activator. Cell 17:131–141
     [Google Scholar]
  35. Quigley J. P., Ossowski L., Reich E. 1974; Plasminogen, the serum proenzyme activated by factors from cells transformed by oncogenic viruses. Journal of Biological Chemistry 249:4306–4311
     [Google Scholar]
  36. Quigley J. P., Goldfarb R. H., Scheiner C., O’Donnel-Tormey J., Yeo T. K. 1980; Plasminogen activator and the membrane of transformed cells. In Tumor Cell Surfaces and Malignancy pp. 773–796 Edited by Hynes R. O., Fox C. F. New York: Alan R. Liss;
     [Google Scholar]
  37. Recklies A. D., Tiltman K. J., Stoker A. M., Poole A. R. 1980; Secretion of proteinases from malignant and non-malignant breast tissue. Cancer Research 40:550–556
     [Google Scholar]
  38. Reich E. 1975; Plasminogen activator: secretion by neoplastic cells and macrophages. In Proteases and Biological Control pp. 333–342 Edited by Reich E., Rifkin D. B., Shaw E. New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  39. Rifkin D. B., Beal L. P., Reich E. 1975; Macromolecular determinants of plasminogen activator synthesis. In Proteases and Biological Control pp. 841–847 Edited by Reich E., Rifkin D. B., Shaw E. New York: Cold Spring Harbor Laboratory;
     [Google Scholar]
  40. Solomon J. A., Chou I. N., Schroder E. W., Black P. H. 1980; Evidence for membrane association of plasminogen activator activity in mouse macrophages. Biochemical and Biophysical Research Communications 94:480–486
     [Google Scholar]
  41. Strickland S., Beers W. H. 1976; Studies on the role of plasminogen activator in ovulation. In vitro response of granulosa cells to gonadotropins, cyclic nucleotides and prostaglandins. Journal of Biological Chemistry 251:5694–5702
     [Google Scholar]
  42. Strickland S., Reich E., Sherman M.I. 1976; Plasminogen activator in early embryogenesis: enzyme production by trophoblast and parietal endoderm. Cell 9:231–240
     [Google Scholar]
  43. Tucker W. S., Kirsch W. M., Martinez-Hernandez A., Fink L. M. 1978; In vitro plasminogen activator activity in human brain tumors. Cancer Research 38:297–302
     [Google Scholar]
  44. Unkeless J. C., Tobia A., Ossowski L., Quigley J. P., Rifkin D. B., Reich E. 1973; An enzymatic function associated with transformation of fibroblasts by oncogenic viruses. I. Chick embryo fibroblast cultures transformed by avian RNA tumor viruses. Journal of Experimental Medicine 137:85–111
     [Google Scholar]
  45. Unkeless J., Dano K., Kellerman G. M., Reich E. 1974; Fibrinolysis associated with oncogenic transformation. Partial purification and characterisation of the cell factor, a plasminogen activator. Journal of Biological Chemistry 249:4295–4305
     [Google Scholar]
  46. Vassalli J. D., Hamilton J., Reich E. 1976; Macrophage plasminogen activator: modulation of enzyme production by anti-inflammatory steroids, mitotic inhibitors and cyclic nucleotides. Cell8271–281
     [Google Scholar]
  47. Werb Z., Aggeler J. 1978; Proteases induce secretion of plasminogen activator by fibroblasts. Proceedings of the National Academy of Sciences of the United States of America 75:1839–1843
     [Google Scholar]
  48. Wigler M., Weinstein I. B. 1976; Tumour promotor induces plasminogen activator. Nature, London 259:232–233
     [Google Scholar]
  49. Wilson E. L., Dowdle E. 1978; Secretion of plasminogen activator by normal reactive and neoplastic human tissues cultured in vitro . International Journal of Cancer 22:309–399
     [Google Scholar]
  50. Wilson E. L., Reich E. 1978; Plasminogen activator in chick fibroblasts: induction of synthesis by retinoic acid: synergism with viral transformation and phorbol esters. Cell 15:385–392
     [Google Scholar]
  51. Wilson E. L., Becker M. L. B., Hoal E. G., Dowdle E. B. 1980; Molecular species of plasminogen activators secreted by normal and neoplastic human cells. Cancer Research 40:933–938
     [Google Scholar]
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Enhancement of Plasminogen Activator Activity by the Culture Medium of Rous Sarcoma Virus-transformed Cells
J. Gen. Virol. 63, 365 (1982); https://doi.org/10.1099/0022-1317-63-2-365
/content/journal/jgv/10.1099/0022-1317-63-2-365
/content/journal/jgv/10.1099/0022-1317-63-2-365
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