1887

Abstract

Summary

We have examined the relationship between induction of interferon (IFN) and prostaglandin E (PGE) production by poly(rI).poly(rC) in cultured human foreskin fibroblasts (FS11). Hydrocortisone and dexamethasone (2.5 × 10 ), which are known inhibitors of PGE synthesis, significantly decreased the induction of both IFN and PGE in IFN-pretreated (primed) cells. Desoxycorticosterone, progesterone and estradiol were devoid of this activity. Hydrocortisone also blocked the induction of IFN by double-stranded RNA (dsRNA), cycloheximide and actinomycin D in FS11 cells. Arachidonic acid overcame the inhibitory effect of hydrocortisone on PGE production, but failed to restore IFN production in the presence of the steroid. Moreover, the prostaglandin synthetase inhibitors, indomethacin, aspirin and flufenamic acid, did not change IFN production by dsRNA in primed FS11 cells, although prostaglandin synthesis was abolished. Although the induction of IFN and PGE by poly(rI).poly(rC) might be consequences of the same initial event in the cell, the accumulation of PGE does not seem to have a regulatory effect on the synthesis of IFN in this system.

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1982-12-01
2024-04-25
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References

  1. Adolf G. R., Swetly P. 1979; Glucocorticoid hormones inhibit DNA synthesis and enhance interferon production in a human lymphoid cell line. Nature, London282736–738
    [Google Scholar]
  2. Armstrong J. A. 1971; Semi-micro dye-binding assay for rabbit interferon. Applied Microbiology21723–725
    [Google Scholar]
  3. Bauminger S., Zor U., Lindner H. R. 1973; Radioimmunological assay of prostaglandin synthetase activity. Prostaglandins4313–324
    [Google Scholar]
  4. Caspi D., Yaron M., Yaron I., Baratz M., Zor U. 1982; Stimulation of prostaglandin E production by rat synovium induced by intraarticular injection of measles virus. Journal of Rheumatology (in press)
    [Google Scholar]
  5. De Maeyer E., De Maeyer J. 1963; Two-sided effect of steroids on interferon in tissue culture. Nature, London197724–725
    [Google Scholar]
  6. Epstein L. B. 1979; The comparative biology of immune and classical interferons. In Biology of the Lymphokines pp. 443–514 Edited by Cohen S., Pick E., Oppenheim J.J. New York: Academic Press;
    [Google Scholar]
  7. Ferraris V. A., Derubertis F. R. 1974; Release of prostaglandin by mitogen-and antigen-stimulated leukocytes in culture. Journal of Clinical Investigation54378–385
    [Google Scholar]
  8. Fitzpatrick F. A., Stringfellow D. A. 1980; Virus and interferon effects on cellular prostaglandin biosynthesis. Journal of Immunology 125:431–437
    [Google Scholar]
  9. Floman Y., Zor U. 1976; Mechanism of steroid action in inflammation: inhibition of prostaglandin synthesis and release. Prostaglandins12403–413
    [Google Scholar]
  10. Gresser I., Tovey M. G. 1978; Antitumor effects of interferon. Biochimica et biophysica acta 516:231–247
    [Google Scholar]
  11. Grossberg S. E. 1972; The interferons and their inducers: molecular and therapeutic considerations. New England Journal of Medicine 287:13–1979–85122–128
    [Google Scholar]
  12. Gryglewski R. J., Panczenko B., Korbut R., Grodzinska L., Ocetkiewicz A. 1975; Corticosteroids inhibit prostaglandin release from perfused mesenteric blood vessels of rabbit and from perfused lungs of sensitized guinea pig. Prostaglandins 10:343–355
    [Google Scholar]
  13. Honn K. V., Bockman R. S., Marnett L. J. 1981; Prostaglandins and cancer: a review of tumor initiation through tumor metastasis. Prostaglandins 21:833–864
    [Google Scholar]
  14. Johnson H. M., Baron S. 1977; Evaluation of effects of interferon and interferon inducers on the immune response. Pharmacology and Therapeutics A 1:349–367
    [Google Scholar]
  15. Kilbourne E. D., Horsfall F. L. Jr. 1951a; Increased virus in eggs injected with cortisone. Proceedings of the Society for Experimental Biology and Medicine 76:116–118
    [Google Scholar]
  16. Kilbourne E. D., Horsfall F. L. Jr. 1951b; Lethal infection with coxsackie virus of adult mice given cortisone. Proceedings of the Society for Experimental Biology and Medicine 77:135–138
    [Google Scholar]
  17. Kilbourne E. D., Smart K. M., Pokorny B. A. 1961; Inhibition by cortisone of the synthesis and action of interferon. Nature, London 190:650–651
    [Google Scholar]
  18. Parks W. P., Ransom J. C., Young H. A., Scolnick E. M. 1975; Mammary tumor virus induction by glucocorticoids. Journal of Biological Chemistry 250:3330–3336
    [Google Scholar]
  19. Pick E. 1977; Lymphokines: physiologic control and pharmacological modulation of their production and action. In Comprehensive Immunology vol. 3 pp. 163–202 Edited by Hadden J. W., Coffey R. G., Spreafico F. New York: Plenum Press;
    [Google Scholar]
  20. Pottathil R., Chandrabose K. A., Cuatrecasas P., Lang D. J. 1980; Establishment of the interferon- mediated antiviral state: role of fatty acid cyclooxygenase. Proceedings of the National Academy of Sciences of the United States of America 77:5437–5440
    [Google Scholar]
  21. Ringold G. M., Shank P. R., Yamamoto K. R. 1978; Production of unintegrated mouse mammary tumor virus DNA in infected rat hepatoma cells is a secondary action of dexamethasone. Journal of Virology 26:93–101
    [Google Scholar]
  22. Roos P., Lindgren J. A., Hammarstrom S. 1980; On the mechanism of elevated prostaglandin E, production in 3T3 fibroblasts transformed by polyoma virus. European Journal of Biochemistry 108:279–283
    [Google Scholar]
  23. Rosztoczy I. 1978; Effect of indomethacin on the antiviral and priming effect of interferon. Acta virologica 22:254
    [Google Scholar]
  24. Santoro M. G., Benedetto A., Carruba G., Garaci E., Jaffe B. M. 1980; Prostaglandin A compounds as antiviral agents. Science 209:1032–1034
    [Google Scholar]
  25. Santoro M. G., Carruba G., Garaci E., Jaffe B. M., Benedetto A. 1981; Prostaglandins of the A series inhibit Sendai virus replication in cultured cells. Journal of General Virology 53:75–83
    [Google Scholar]
  26. Stanley E. D., Jackson G. G., Panusarn C., Rubenis M., Dirda V. 1975; Increased virus shedding with aspirin treatment of rhinovirus infection. Journal of the American Medical Association 231:1248–1251
    [Google Scholar]
  27. Stringfellow D. A. 1978; Prostaglandin restoration of the interferon response of hyporeactive animals. Science 201:376–378
    [Google Scholar]
  28. Vilcek J., Havell E. A. 1973; Stabilization of interferon messenger RNA activity by treatment of cells with metabolic inhibitors and lowering of the incubation temperature. Proceedings of the National Academy of Sciences of the United States of America 70:3909–3913
    [Google Scholar]
  29. Wallach D., Revel M. 1979; Hormonal protection of interferon-treated cells against double-stranded RNA- induced cytolysis. FEBS Letters 101:364–368
    [Google Scholar]
  30. Wu A. M., Schultz A., Gallo R. C. 1976; Synthesis of type C virus particles from murine-cultured cell induced by iododeoxyuridine. V. Effect of interferon and its interaction with dexamethasone. Journal of Virology 19:108–117
    [Google Scholar]
  31. Yaron M., Yaron I., Gurari-Rotman D., Revel M., Lindner H. R., Zor U. 1977; Stimulation of prostaglandin E production in cultured human fibroblasts by poly(I). poly(C) and human interferon. Nature, London 267:457–459
    [Google Scholar]
  32. Yaron M., Yaron I., Wiletzki C., Zor U. 1978; Interrelationship between stimulation of prostaglandin E and hyaluronate production by poly(I).poly(C) and interferon in synovial fibroblast culture. Arthritis and Rheumatism 21:694–698
    [Google Scholar]
  33. Yaron M., Yaron I., Caspi D., Smetana O., Eylan E., Zor U. 1981; RNA and DNA viral stimulation of prostaglandin E production by human synovial fibroblasts. Arthritis and Rheumatism 24:1582–1586
    [Google Scholar]
  34. Young H. A., Shih T. Y., Scolnick E. M., Parks W. P. 1977; Steroid induction of mouse mammary tumor virus: effect upon synthesis and degradation of viral RNA. Journal of Virology 21:139–146
    [Google Scholar]
  35. Zor U., Lamprecht S. A. 1977; Mechanism of prostaglandin action in endocrine glands. In Biochemical Action of Hormones vol. 4 pp. 85–133 Edited by Litwack G. New York & London: Academic Press;
    [Google Scholar]
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