1887

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Volume 63, Issue 2

Characterization of Non-oncogenic Marek's Disease Virus-infected and Turkey Herpesvirus-infected Lymphocytes Free

Abstract

Summary

Splenic leukocytes derived from N- and P-line chickens exposed to turkey herpesvirus (HVT-4), and from UCD-003 chickens exposed to the non-oncogenic SB-1 clone of Marek's disease virus were fractionated into subpopulations at various days post-exposure. The level of infection in these fractions was determined by enumeration of plaque-forming units after co-cultivation of leukocytes with virus-permissive chicken embryo fibroblasts. At 5 days post-exposure most HVT-4-infected lymphocytes were found to be of intermediate to low buoyant density (1.040 to 1.065 g/ml) and not to have detectable Fc receptors. They possessed surface Ia but were not IgM-bearing. At 15 days post-exposure HVT-4-infected lymphocytes were found to be of intermediate to high buoyant density (1.070 to 1.080 g/ml) and, at this time, virus isolation rates (p.f.u./10 cells) from fractions enriched in cells bearing Fc receptors were approximately equal to those leukocyte suspensions depleted of Fc receptor-bearing cells. Furthermore, the percentage reduction in infectivity caused by depletion of Ia-bearing cells at 15 days post-exposure was less than that at 5 days post-exposure. At both 5 and 15 days post-exposure carbonyl iron treatment failed to remove HVT-4-infected lymphocytes. These characteristics were similar whether HVT-4-infected lymphocytes were derived from N- or P-line chickens. SB-1-infected lymphocytes derived from UCD-003 chickens at 6, 7 and 14 days post-exposure were detected with equal frequency in fractions enriched or depleted of cells possessing Fc receptors. SB-1-infected lymphocytes for the most part lacked surface Ia and IgM, and were not depleted by carbonyl iron treatment. From these data it was concluded that most HVT-4- and SB-1-infected lymphocytes detectable by virus isolation were neither B cells nor macrophages.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): lymphocytes , MDV and turkey herpesvirus
© Society for General Microbiology, 1982
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1982-12-01
2024-04-20
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Characterization of Non-oncogenic Marek's Disease Virus-infected and Turkey Herpesvirus-infected Lymphocytes
J. Gen. Virol. 63, 333 (1982); https://doi.org/10.1099/0022-1317-63-2-333
/content/journal/jgv/10.1099/0022-1317-63-2-333
/content/journal/jgv/10.1099/0022-1317-63-2-333
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