1887

Abstract

Summary

The production and properties of three monoclonal antibodies, designated LP1, LP4 and AP2, directed against non-glycosylated polypeptides of herpes simplex virus type 2 are described. LP1 is specific for polypeptide VP16 and cross-reacts with HSV-1; LP4 reacts with the major DNA-binding protein and is type-specific. AP2 is directed against the major capsid antigen of HSV-1 and HSV-2.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-63-2-297
1982-12-01
2021-10-20
Loading full text...

Full text loading...

/deliver/fulltext/jgv/63/2/JV0630020297.html?itemId=/content/journal/jgv/10.1099/0022-1317-63-2-297&mimeType=html&fmt=ahah

References

  1. Anzai T., Dreesman G. R., Courtney R. J., Adam E., Rawls W. E., Benyesh-Melnick M. 1975; Antibodies to herpes simplex virus type-2 induced non-structural proteins in women with cervical cancer and control groups. Journal of the National Cancer Institute 54:1051–1059
    [Google Scholar]
  2. Balachandran N., Harnish D., Killington R. A., Bachetti S., Rawls W. E. 1981; Monoclonal antibodies to two glycoproteins of herpes simplex virus type 2. Journal of Virology 39:438–446
    [Google Scholar]
  3. Baucke R. B., Spear P. G. 1979; Membrane proteins specified by herpes simplex viruses. V. Identification of Fc binding glycoprotein. Journal of Virology 32:779–789
    [Google Scholar]
  4. Cassai E. N., Sarmiento M., Spear P. G. 1975; Comparison of the virion proteins specified by herpes simplex virus types 1 and 2. Journal of Virology 16:1327–1331
    [Google Scholar]
  5. Chen M. S., Prusoff W. H. 1978; Association of thymidilate kinase with pyrimidine nucleoside kinase induced by herpes simplex virus. Journal of Biological Chemistry 253:1325–1327
    [Google Scholar]
  6. Dreesman G. R., Burek J., Adam E., Kaufman R. H., Melnick I. L., Powell K. L., Purifoy D. J. 1980; Expression of herpes virus induced antigens in human cervical cancer. Nature, London 283:591–593
    [Google Scholar]
  7. Dubbs D. R., Kit S. 1964; Mutant strains of herpes simplex deficient in thymidine kinase inducing activity. Virology 22:493–502
    [Google Scholar]
  8. Eberle R., Courtney R. J. 1980; gA and gB glycoproteins of herpes simplex type 1: two forms of a single polypeptide. Journal of Virology 36:665–675
    [Google Scholar]
  9. Ejercito P. M., Kieff E. D., Roizman B. 1968; Characterization of herpes simplex virus strains differing in their effects on the social behaviour of infected cells. Journal of General Virology 2357–364
    [Google Scholar]
  10. Ey P. L., Prowse S. J., Jenkin C. R. 1978; Isolation of pure IgGl, IgG2a, and IgG2b immunoglobulins from mouse serum using protein A sepharose. Immunochemistry 15:429–436
    [Google Scholar]
  11. Flannery V. L., Courtney R. J., Schaffer P. A. 1977; Expression of an early non-structural antigen of herpes simplex virus in cells transformed by herpes simplex virus. Journal of Virology 21:284–291
    [Google Scholar]
  12. Galfre G., Howe S. C., Milstein C. 1977; Antibodies to major histocompatibility antigens produced by a hybrid cell line. Nature, London 266:550–552
    [Google Scholar]
  13. Gibson W., Roizman B. 1974; Proteins specified by herpes simplex virus. X. Staining and radiolabelling properties of B capsid and virion proteins in polyacrylamide gels. Journal of Virology 13:155–165
    [Google Scholar]
  14. Haarr L., Marsden H. S. 1981; Two dimensional gel analysis of HSV type 1 induced polypeptides and glycoprotein processing. Journal of General Virology 52:77–92
    [Google Scholar]
  15. Heine J. W., Honess R. W., Cassai E., Roizman B. 1974; Proteins specified by herpes simplex virus. The virion polypeptides of type 1 strains. Journal of Virology 14:640–651
    [Google Scholar]
  16. Heusser C. H., Stocker J. W., Gisler R. H. 1981; Solid-phase immunoassay for cell antigens. Methods in Enzymology 73:406–418
    [Google Scholar]
  17. Hill T. J., Field H. J., Blyth W. A. 1975; Acute and recurrent infection with herpes simplex virus in the mouse: a model for studying latency and recurrent disease. Journal of General Virology 28:341–353
    [Google Scholar]
  18. Honess R. W., Roizman B. 1973; Proteins specified by herpes simplex virus. XI: Identification and relative molar rates of synthesis of structural and non-structural herpes virus polypeptides in the infected cell. Journal of Virology 12:1347–1365
    [Google Scholar]
  19. Killington R. A., Yeo J., Honess R. W., Watson D. H., Duncan B. E., Halliburton I. W., Mumford J. 1977; Comparative analysis of the proteins and antigens of five herpesviruses. Journal of General Virology 37:297–310
    [Google Scholar]
  20. Knopf K. W., Kaerner H. C. 1980; Virus specific basic phosphoproteins associated with simplex virus type 1 particles and the chromatin of HSV-1 infected cells. Journal of General Virology 46:405–438
    [Google Scholar]
  21. Kronvall G., Grey H. M., Williams R. C. 1970; Protein A reactivity with mouse immunoglobulins. Structural relationships between some mouse and human immunoglobulins. Journal of Immunology 105:116–127
    [Google Scholar]
  22. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  23. Littlefield J. 1964; Selection of hybrids from matings by fibroblasts in vitro and their presumed recombinants. Science 145:709–710
    [Google Scholar]
  24. Littler E., Yeo J., Killington R. A., Purifoy D. J., Powell K. L. 1981; Antigenic and structural conservation of herpesvirus DNA binding proteins. Journal of General Virology 56:409–419
    [Google Scholar]
  25. Marsden H. S., Crombie I. K., Subak-Sharpe J. H. 1976; Control of protein synthesis in herpes virus infected cells: analysis of the polypeptides induced by wild type and sixteen temperature sensitive mutants of HSV-1 strain 17. Journal of General Virology 31:347–372
    [Google Scholar]
  26. Marsden H. S., Stow N. D., Preston V. G., Timbury M. C., Wilkie N. M. 1978; Physical mapping of herpes simplex virus induced polypeptides. Journal of Virology 28:624–642
    [Google Scholar]
  27. Melnick J., Courtney R. J., Powell K. L., Schaffer P. A., Benyesh-Melnick M., Dreesman G. R., Anzai T., Adam E. 1976; Studies on herpes simplex virus and cancer. Cancer Research 36:845–846
    [Google Scholar]
  28. Pereira L., Klassen T., Baringer J. R. 1980; Type common and type specific monoclonal antibody to herpes simplex virus. Infection & Immunity 29:724–732
    [Google Scholar]
  29. Pereira L., Dondero D. V., Gallo D., Devlin V., Woodie J. D. 1982; Serological analysis of herpes simplex virus types 1 and 2 with monoclonal antibodies. Infection & Immunity 35:363–367
    [Google Scholar]
  30. Powell K. L., Courtney R. J. 1975; Polypeptides synthesized in herpes simplex virus type 2 infected HEp-2 cells. Virology 66:217–228
    [Google Scholar]
  31. Powell K. L., Purifoy D. J. 1977; Non-structural proteins of herpes simplex virus. I: Purification of the induced DNA polymerase. Journal of Virology 24:618–626
    [Google Scholar]
  32. Powell K. L., Watson D. H. 1975; Some structural antigens of herpes simplex virus type 1. Journal of General Virology 29:167–178
    [Google Scholar]
  33. Powell K. L., Littler E., Purifoy D. J. 1981; Non-structural proteins of herpes simplex virus. II: Major virus- specific DNA binding protein. Journal of Virology 39:894–902
    [Google Scholar]
  34. Purifoy D. J., Powell K. L. 1976; DNA binding proteins induced by herpes simplex type 2 in HEp-2 cells. Journal of Virology 19:717–731
    [Google Scholar]
  35. Showalter S. D., Zweig M., Hampar B. 1981; Monoclonal antibodies to herpes simplex virus type 1 proteins, including the immediate early protein ICP4. Infection & Immunity 34:684–692
    [Google Scholar]
  36. Spear P. G., Roizman B. 1972; Proteins specified by herpes simplex virus. V: Purification and structural proteins of the herpesvirion. Journal of Virology 9:143–159
    [Google Scholar]
  37. Strobel-Fidler M., Francke B. 1980; Alkaline deoxyribonuclease induced by herpes simplex virus type 1: composition and properties of the purified enzyme. Virology 103:493–501
    [Google Scholar]
  38. Thouless M. E., Skinner G. R. B. 1971; Differences in the properties of thymidine kinase produced in cells infected with type 1 or type 2 herpes virus. Journal of General Virology 12:195–197
    [Google Scholar]
  39. Watson D. H., Wildy P. 1969; The preparation of'monoprecipitin’ antisera of herpes virus specific antigens. Journal of General Virology 4:163–168
    [Google Scholar]
  40. Watson D. H., Shedden W. I. H., Elliot A., Tetsuka T., Wildy P., Bourgaux-Ramoisy D., Gold E. 1966; Virus specific antigens in mammalian cells infected with herpes simplex virus. Immunology 11:399–408
    [Google Scholar]
  41. Yeo J., Killington R. A., Watson D. H., Powell K. L. 1981; Studies on cross-reactive antigens in herpesviruses. Virology 108:256–266
    [Google Scholar]
  42. Zweig M., Heilman C. J., Rabin H., Hopkins R. F., Neubauer R. H., Hampar B. 1979; Production of monoclonal antibodies against nucleocapsid proteins of herpes simplex virus types 1 and 2. Journal of Virology 32:676–678
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-63-2-297
Loading
/content/journal/jgv/10.1099/0022-1317-63-2-297
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error