1887

Abstract

SUMMARY

The ability of adenovirus structural polypeptides to bind nucleic acids was assessed by separating the polypeptides on SDS-polyacrylamide gels, transferring them electrophoretically to nitrocellulose and probing with P-labelled nucleic acids. Polypeptides IVa, V, VI and VII, as well as trace amounts of pVII and a polypeptide of apparent mol. wt. 40 × 10 were able to bind label under these conditions. Labelling was also detected with a smaller polypeptide, possibly related to the cleavage products of pVII and/or pVI. The binding of to polypeptide VI appeared to be more sensitive to detergents than the others. No sequence specificity could be detected in the binding.

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1982-11-01
2022-10-06
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References

  1. Akusjarvi G., Persson H. 1981; Gene and mRNA for precursor polypeptide VI from adenovirus type 2. Journal of Virology 38:469–482
    [Google Scholar]
  2. Anderson C. W., Baum P. R., Gesteland R. F. 1973; Processing of adenovirus 2 induced proteins. Journal of Virology 12:241–252
    [Google Scholar]
  3. Anderson W. F., Ohlerdorf D. H., Takeda Y., Mathews B. W. 1981; Structure of the crorepressor from bacteriophage λ and its interaction with DNA. Nature, London 290:754–758
    [Google Scholar]
  4. Bowen B., Steinberg J., Laemmli U. K., Weintraub H. 1980; The detection of DNA binding proteins by protein blotting. Nucleic Acids Research 8:1–20
    [Google Scholar]
  5. Bradley M. K., Griffin J. D., Livingston D. M. 1982; Relationship of oligomerization to enzymatic and DNA binding properties of the SV40 large T antigen. Cell 28:125–134
    [Google Scholar]
  6. Casjens S., King J. 1975; Virus assembly. Annual Review of Biochemistry 44:555–611
    [Google Scholar]
  7. Chou P. Y., Fasman G. D. 1978; Prediction of the secondary structure of proteins from their amino acid sequence. Advances in Enzymology 47:45–148
    [Google Scholar]
  8. Clarke S. 1981; Direct renaturation of the dodecyl sulfate complexes of proteins with Triton X-100. Biochimica et biophysica acta 670:195–202
    [Google Scholar]
  9. D’Halluin J.-C., Milleville M., Boulanger P. A., Martin G. R. 1978a; Temperature sensitive mutant of adenovirus type 2 blocked in virion assembly: accumulation of light intermediate particles. Journal of Virology 26:344–356
    [Google Scholar]
  10. D’Halluin J.-C., Martin G. R., Torpier G., Boulanger P. A. 1978b; Adenovirus type 2 assembly analysed by reversible cross linking of labile intermediates. Journal of Virology 26:357–363
    [Google Scholar]
  11. Dimitriadis G. J. 1979; Effect of detergents on antibody antigen interaction. Analytical Biochemistry 98:445–451
    [Google Scholar]
  12. Edvardsson B., Everitt E., Jornvall H., Prage L., Philipson L. 1976; Intermediates in adenovirus assembly. Journal of Virology 19:533–547
    [Google Scholar]
  13. Everitt E., Lutter L., Philipson L. 1975; Structural proteins of adenoviruses. XII. Location and neighbor relationship among proteins of adenovirus type 2 as revealed by enzymatic iodination, immunoprecipitation and chemical cross-linking. Virology 52:130–147
    [Google Scholar]
  14. Feiss M., Fisher R. A., Siegele D. A., Nichols B. P., Donelson J. E. 1979; Packaging of the bacteriophage lambda chromosome: a role for the base sequences outside cos. Virology 92:56–67
    [Google Scholar]
  15. Flint S. J. 1980; Structure and genome organisation of adenoviruses. In DNA Tumor Viruses pp. 383–441 Edited by Tooze J. New York: Cold Spring Harbor Laboratory;
    [Google Scholar]
  16. Geisow M. J., Roberts R. D. B. 1980; Amino acid preferences for secondary structure vary with protein class. International Journal of the Biology of Macromolecules 2:387–389
    [Google Scholar]
  17. Ginsberg H. S., Pereira H. G., Valentine R. C., Wilcox W. C. 1966; A proposed terminology for the adenovirus antigens and virion morphological subunits. Virology 28:782–783
    [Google Scholar]
  18. Green M., Pina M. 1963; Biochemical studies on adenovirus multiplication. IV. Isolation, purification and chemical analysis of adenovirus. Virology 20:199–209
    [Google Scholar]
  19. Hammarskjold M., -L. Winberg G. 1980; Encapsidation of adenovirus 16 DNA is directed by a small DNA sequence at the left end of the genome. Cell 20:787–795
    [Google Scholar]
  20. Ishibashi M., Maizel J. V. Jr 1974; The polypeptides of adenovirus. V. Bound virions, structural intermediates between top components and aged virions. Virology 57:409–424
    [Google Scholar]
  21. Langridge J., Langridge P., Bergquist P. L. 1980; Extraction of nucleic acids from agarose gels. Analytical Biochemistry 103:264–271
    [Google Scholar]
  22. Laskey R. A., Mills A. D. 1977; Enhanced autoradiographic detection of 32P and 125I using intensifying screens and hypersensitized film. FEBS Letters 82:314–316
    [Google Scholar]
  23. Laver W. G., Pereira H. G., Russell W. C., Valentine R. C. 1968; Isolation of an internal component from adenovirus type 5. Journal of Molecular Biology 37:379–386
    [Google Scholar]
  24. Mckay D. B., Steitz T. A. 1981; Structure of catabolite gene activator protein at 2.9 A resolution suggests binding to left-handed B-DNA. Nature, London 290:744–749
    [Google Scholar]
  25. Maxam A. M., Gilbert W. 1977; A new method for sequencing DNA. Proceedings of the National Academy of Sciences of the United States of America 74:560–564
    [Google Scholar]
  26. Myers R. M., Williams R. C., Tjian R. 1981; Oligomeric structure of a simian virus 40 T antigen in free form and bound to DNA. Journal of Molecular Biology 148:347–353
    [Google Scholar]
  27. Nermut M. V. 1980; The architecture of adenoviruses: recent views and problems. Archives of Virology 64:175–196
    [Google Scholar]
  28. Persson H., Mathison B., Philipson L., Pettersson U. 1979; A maturation protein in adenovirus morphogenesis. Virology 93:198–208
    [Google Scholar]
  29. Prage L., Pettersson U., Philipson L. 1968; Internal basic proteins in adenovirus. Virology 36:508–514
    [Google Scholar]
  30. Rekosh D. M. K., Russell W. C., Bellett A. J. D., Robinson A. J. 1977; Identification of a protein linked to the ends of adenovirus DNA. Cell 11:283–295
    [Google Scholar]
  31. Rigby P. W. J., Dieckmann M., Rhodes C., Berg P. 1977; Labelling deoxyribonucleic acid to high specific activity in vitroby nick translation with DNA polymerase I. Journal of Molecular Biology 113:237–251
    [Google Scholar]
  32. Russell W. C., Blair G. E. 1977; Polypeptide phosphorylation in adenovirus infected cells. Journal of General Virology 34:19–35
    [Google Scholar]
  33. Russell W. C., Winters W. D. 1975; Assembly of viruses. Progress in Medical Virology 19:1–39
    [Google Scholar]
  34. Russell W. C., Hayashi K., Sanderson P. J., Pereira H. G. 1967; Adenovirus antigens - a study of their properties and sequential development in infection. Journal of General Virology 1:495–508
    [Google Scholar]
  35. Russell W. C., Laver W. G., Sanderson P. J. 1968; The internal components of adenovirus. Nature, London 219:1127–1129
    [Google Scholar]
  36. Russell W. C., Mcintosh K., Skehel J. J. 1971; The preparation and properties of adenovirus cores. Journal of General Virology 11:35–45
    [Google Scholar]
  37. Skehel J. J., Hay A. J. 1978; Nucleotide sequences at the 5' termini of influenza virus RNA’s and their transcripts. Nucleic Acids Research 5:1207–1219
    [Google Scholar]
  38. Tibbetts C. 1977; Viral DNA sequences from incomplete particles of human adenovirus type 7. Cell 12:243–249
    [Google Scholar]
  39. Tibbetts C., Giam C.-Z. 1979; In vitroassociation of empty adenovirus capsids with double stranded DNA. Journal of Virology 32:995–1005
    [Google Scholar]
  40. Towbin H., Staehlin T., Gordon J. 1979; Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences of the United States of America 76:4350–4354
    [Google Scholar]
  41. Van Beveren C. P., Maat J., Dekker B. M. M., Van Ormondt H. 1981; The nucleotide sequence of the gene for protein IVa2 and of the 5' leader segment of the major late mRNAs of adenovirus type 5. Gene 16:179–189
    [Google Scholar]
  42. Weber J. 1976; Genetic analysis of adenovirus type 2. III. Temperature sensitivity of processing of viral proteins. Journal of Virology 17:462–471
    [Google Scholar]
  43. Weber K., Kuter D. J. 1971; Reversible denaturation of enzymes by sodium dodecyl sulphate. Journal of Biological Chemistry 246:4504–4509
    [Google Scholar]
  44. Winter G., Fields S., Gait M. J., Brownlee G. G. 1981; The use of synthetic oligonucleotide primers in cloning and sequencing segment 8 of influenza virus (A/PR/8/34). Nucleic Acids Research 9:237–241
    [Google Scholar]
  45. Winters W. D., Russell W. C. 1971; Studies on the assembly of adenovirus in vitro. Journal of General Virology 10:118–194
    [Google Scholar]
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