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Journal of General Virology header logo Journal of General Virology

Volume 63, Issue 1

Nucleic Acid-binding Properties of Adenovirus Structural Polypeptides No Access

Abstract

SUMMARY

The ability of adenovirus structural polypeptides to bind nucleic acids was assessed by separating the polypeptides on SDS-polyacrylamide gels, transferring them electrophoretically to nitrocellulose and probing with P-labelled nucleic acids. Polypeptides IVa, V, VI and VII, as well as trace amounts of pVII and a polypeptide of apparent mol. wt. 40 × 10 were able to bind label under these conditions. Labelling was also detected with a smaller polypeptide, possibly related to the cleavage products of pVII and/or pVI. The binding of to polypeptide VI appeared to be more sensitive to detergents than the others. No sequence specificity could be detected in the binding.

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Keyword(s): Adenovirus structural polypeptides , electrophoretic blotting and radiolabelled probes
© Society for General Microbiology 1982
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/content/journal/jgv/10.1099/0022-1317-63-1-69
1982-11-01
2026-02-19

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/content/journal/jgv/10.1099/0022-1317-63-1-69
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Nucleic Acid-binding Properties of Adenovirus Structural Polypeptides
J. Gen. Virol. 63, 69 (1982); https://doi.org/10.1099/0022-1317-63-1-69
/content/journal/jgv/10.1099/0022-1317-63-1-69
/content/journal/jgv/10.1099/0022-1317-63-1-69
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