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Volume 63, Issue 1

Rauscher Spleen Focus-forming Virus: Biological Properties and Relationship to Helper Viruses Free

Abstract

SUMMARY

A Rauscher virus (RV)-transformed erythroid cell line, RA-1, was shown to be a non-producer cell line. RA-1 cells express not only gp51–54 -related glycoprotein, but also gp70, which is more closely related to gp51–54 coded by a recombinant gene than to the gp70. RA-1 cells could be infected by Friend, Moloney and Gross viruses, but not by the homologous Rauscher murine leukaemia virus. Rescue of spleen focus-forming activity was obtained on infection of these cells with or , but not with -Gross. The of the RV complex resembles closely that of Friend virus (). It contains a 32S, presumably defective, genome, which most likely is responsible for spleen focus formation, and a 35S helper virus genome. Oligonucleotide fingerprint data suggest that RV has evolved independently of . Erythroid early BFU-E cells of mice infected with RV of Friend helper virus-infected RA-1 cells were shown to require no addition of conditioned medium to form large erythroid colonies (BFU-E) in the presence of only small amounts of erythropoietin.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): BPA independence , Friend virus , Rauscher virus and spleen focus-forming virus
© Society for General Microbiology 1982
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1982-11-01
2024-04-19
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Rauscher Spleen Focus-forming Virus: Biological Properties and Relationship to Helper Viruses
J. Gen. Virol. 63, 45 (1982); https://doi.org/10.1099/0022-1317-63-1-45
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