1887

Abstract

SUMMARY

The biological activity of a molecularly cloned of a rat endogenous C-type leukaemia helper virus, , was assessed by intranuclear microinjection into normal rat kidney cells ( ). Release of rat C-type leukaemia helper viruses by the microinjected cells was examined by superinfection of Kirsten-transformed non-producer cells (K-). Immediate release of helper leukaemia viruses at a very low level was observed only in the cells microinjected with the supercoiled form of , suggesting that the circular form of the virus might have expedited the replication and expression of virus particles. Genome rescue experiments were also performed by co-cultivating the microinjected cells carrying various linear s, or of subgenomic sizes, with K- cells. The results indicated that both the total and the 5.8 to 6.2 kb fragment proximal to the 5′ terminus of the cloned 8.8 kb were able to rescue successfully a transforming replication-competent pseudotype virus. Subgenomic fragments derived from the centre or the 3′ end of the were ineffective in the genome rescue experiments.

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/content/journal/jgv/10.1099/0022-1317-63-1-37
1982-11-01
2022-10-03
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