1887

Abstract

SUMMARY

Ultraviolet irradiation of murine cytomegalovirus (MCMV) caused a rapid dose-related decline in virus infectivity, manifested by virus antigen induction, and in virus production as measured by plaque formation and infectious centre assay. The virus survival curve was multi-component, suggesting host cell-assisted reactivation. Multiplicity reactivation and photo reactivation of MCMV were not observed in these experiments. Productive infection was more sensitive to u.v. irradiation than was virus antigen production, indicating differential inactivation of virus functions. The effects of u.v. irradiation were similar in most respects to those reported for human cytomegalovirus.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-63-1-251
1982-11-01
2024-04-18
Loading full text...

Full text loading...

/deliver/fulltext/jgv/63/1/JV0630010251.html?itemId=/content/journal/jgv/10.1099/0022-1317-63-1-251&mimeType=html&fmt=ahah

References

  1. Abel P. 1962; Multiplicity reactivation and marker rescue with vaccinia virus. Virology 17:511–519
    [Google Scholar]
  2. Albrecht T., Rapp F. 1973; Malignant transformation of hamster embryo fibroblasts following exposure to ultraviolet irradiated human cytomegalovirus. Virology 55:53–61
    [Google Scholar]
  3. Albrecht T., St Jeor S. C., Funk F., Rapp F. 1974; Multiplicity reactivation of human cytomegalovirus inactivated by ultraviolet light. International Journal of Radiation Biology 26:445–454
    [Google Scholar]
  4. Boldogh I., Gonczol E., Gartner L., Vaczi L. 1978; Stimulation of host DNA synthesis and induction of early antigens by ultraviolet light irradiated human cytomegalovirus. Archives of Virology 58:289–299
    [Google Scholar]
  5. Davis D. B., Kingsbury D. T. 1976; DNA present in cells transformed by UV irradiated herpes simplex virus. Journal of Virology 17:788–793
    [Google Scholar]
  6. Duff R., Rapp F. 1971; Properties of hamster embryo fibroblasts transformed in vitro after exposure to ultraviolet irradiated herpes simplex virus, type 2. Journal of Virology 8:469–477
    [Google Scholar]
  7. Dulbecco R. 1978 Organization, alteration and expression of gene information. In Microbiology184–213 Edited by Davis B. D., Dulbecco R., Eisen H. N., Ginsberg H. S. Hagerstown, Pennsylvania: Harper & Row;
    [Google Scholar]
  8. Hlrai K., Maeda F., Watanabe Y. 1977; Expression of early virus functions in human cytomegalovirus infected HEL cells: effects of ultraviolet light irradiation of the virus. Journal of General Virology 38:121–133
    [Google Scholar]
  9. Hudson J. B., Misra V., Mosmann T. R. 1976; Cytomegalovirus infectivity: analysis of the phenomenon of centrifugal enhancement of infectivity. Virology 72:235–243
    [Google Scholar]
  10. Jagger J. 1958; Photoreactivation. Bacteriological Reviews 22:99–142
    [Google Scholar]
  11. Jordan M. C., Shanley J. D., Stevens J. G. 1977; Immunosuppression reactivates and disseminates latent murine cytomegalovirus. Journal of General Virology 37:419–423
    [Google Scholar]
  12. Lussier G. 1975; Murine cytomegalovirus. Advances in Veterinary Science and Comparative Medicine 19:223–247
    [Google Scholar]
  13. Lytle C. D. 1971; Host-cell reactivation in mammalian cells. I. Survival of ultraviolet irradiated herpes virus in different cell lines. International Journal of Radiation Biology 19:329–337
    [Google Scholar]
  14. Mannini A., Medearis D. N. 1961; Mouse salivary gland virus infections. American Journal of Hygiene 73:329–343
    [Google Scholar]
  15. Moon H. M., Sapienza V. J., Carp R. I., Kim K. S. 1976; DNA synthesis in mouse embryo fibroblast cells infected with murine cytomegalovirus. Virology 75:376–383
    [Google Scholar]
  16. Pfefferkorn E. R., Coady H. M. 1968; MEChanism of photo reactivation of pseudo rabies virus. Journal of Virology 2:474–479
    [Google Scholar]
  17. Pfefferkorn E. R., Rutstein C., Burge B. W. 1965; Photoreactivation of pseudo rabies virus. Virology 27:457–459
    [Google Scholar]
  18. Ross L. J. N., Cameron K. R., Wildy K. R. 1972; Ultraviolet irradiation of herpes simplex virus: reactivation processes and delay in virus multiplication. Journal of General Virology 16:299–311
    [Google Scholar]
  19. Sairenj T., Hinuma Y. 1975; Ultraviolet inactivation of Epstein-Barr virus: effect on synthesis of virus associated antigens. International Journal of Cancer 16:1–6
    [Google Scholar]
  20. Selgrade M. K., Osborne J. E. 1974; Role of macrophages in resistance to murine cytomegalovirus. Infection and Immunity 10:1383–1390
    [Google Scholar]
  21. Sethi K. K., Brandis H. 1979; Induction of virus specific and H-2 restricted cytotoxic T cells by UV inactivated murine cytomegalovirus. Archives of Virology 60:227–238
    [Google Scholar]
  22. Setlow R. B., Carrier W. L. 1962; Evidence that ultraviolet induced thymine dimers in DNA cause biological damage. Proceedings of the National Academy of Sciences of the United States of America 43:1250–1257
    [Google Scholar]
  23. Setlow R. B., Carrier W. L. 1964; The disappearance of thymine dimers from DNA: an error correcting mecanism. Proceedings of the National Academy of Sciences of the United States of America 51:226–231
    [Google Scholar]
  24. Shanley J. D., Jordan M. C., Cook M. L., Stevens J. G. 1979; Pathogenesis of reactivated latent murine cytomegalovirus infection. American Journal of Pathology 95:67–78
    [Google Scholar]
  25. Zavadova Z., Zavada J. 1968; Host cell repair of ultraviolet irradiated pseudorabies virus in chick embryo cells. Act a virology 12:507–514
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-63-1-251
Loading
/content/journal/jgv/10.1099/0022-1317-63-1-251
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error