Synthesis of a Unique Polypeptide in Measles Virus-infected BGM Cells Free

Abstract

SUMMARY

In measles virus-infected BGM (African green monkey kidney) cells, a unique polypeptide (84K) was identified. This polypeptide was not found in other cell lines examined and could be immunoprecipitated with anti-NP monoclonal antibody. Peptide mapping studies confirmed that it contained all the peptides of the 60K plus several additional ones. Pulse-chase experiments failed to establish it as a precursor of the 60K polypeptide. The possible origin of this protein is discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-63-1-247
1982-11-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/63/1/JV0630010247.html?itemId=/content/journal/jgv/10.1099/0022-1317-63-1-247&mimeType=html&fmt=ahah

References

  1. Fujinami R. S., Oldstone M. B. A. 1981; Failure to cleave measles virus fusion protein in lymphoid cells. Journal of Experimental Medicine 154:1489–1499
    [Google Scholar]
  2. Gibson W. 1974; Polyoma virus proteins: a description of the structural proteins of the virion based on polyacrylamide gel electrophoresis and peptide analysis. Virology 62:319–336
    [Google Scholar]
  3. Glraudon P., Wild T. F. 1981; Monoclonal antibodies against measles virus. Journal of General Virology 54:325–332
    [Google Scholar]
  4. Hardwick J. M., Bussell R. H. 1978; Glycoproteins of measles virus under reducing and non-reducing conditions. Journal of Virology 25:678–692
    [Google Scholar]
  5. Herman R. C., Schubert M., Keene J. D., Lazzarini R. A. 1980; Polycistronic vesicular stomatitis virus RNA transcripts. Proceedings of the National Academy of Sciences of the United States of America 77:4662–4665
    [Google Scholar]
  6. Joseph B. S., Lampert P. W., Oldstone M. B. A. 1975; Replication and persistence of measles virus in defined sub-populations of human leukocytes. Journal of Virology 16:1638–1649
    [Google Scholar]
  7. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  8. Mottet N. K., Szanton V. 1961; Exfoliated measles giant cells in nasal secretions. Archives of Pathology 72:434–437
    [Google Scholar]
  9. Seifreid A. S., Albrecht P., Milstein J. B. 1978; Characterisation of an RNA polymerase activity associated with measles virus. Journal of Virology 25:781–787
    [Google Scholar]
  10. Stallcup K. C., Fields B. N. 1981; The replication of measles virus in the presence of tunicamycin. Virology 108:391–404
    [Google Scholar]
  11. Zweig M., Heilman C. J., Rabin H., Hampar B. 1980; Shared antigenic determinants between two distinct classes of proteins in cells infected with herpes simplex virus. Journal of Virology 35:644–652
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-63-1-247
Loading
/content/journal/jgv/10.1099/0022-1317-63-1-247
Loading

Data & Media loading...

Most cited Most Cited RSS feed