We have examined the effects of heat and several chemical inactivating agents on the buoyant density, particle-associated polypeptides and ultrastructure of reovirus particles. Treatment at pH 11 removed polypeptide σl from the outer capsid of reovirus type 2 but not from type 1; resultant particles were unchanged in their buoyant density and morphology. Treatment of reovirus types 2 and 3 with 2.5 M-guanidine-HCl produced particles with unchanged polypeptide content but an increased buoyant density, and caused aggregation of type 3 but not type 2. Treatment with 1 % SDS removed polypeptide σ3 from both types 1 and 2 and increased the buoyant density of the virus particles. The outer capsid of SDS-treated virions was greatly altered and often indistinct. Treatment of type 3 with either 1% phenol or 33% ethanol produced particles that had a full complement of polypeptides, were unaltered in buoyant density, but were greatly aggregated. Thus, these inactivating agents affect reovirus particles in specific and distinct ways. The differential effects of such treatments can thus be used to study the structure and function of the reovirus capsid components.
FieldsB. N.,
RaineC.,
BaumS. G.1971; Temperature sensitive mutants of reovirus type 3: defects in virus maturation as studied by immunofluorescence and electron microscopy. Virology 43:569–578
HuismansH.,
AjoklikW. K.1976; Reovirus coded polypeptides in infected cells: isolation of two native monomeric polypeptides with affinity for single-stranded and double-stranded RNA, respectively. Virology 70:411–424
LeeP. K. W.,
HayesE. C.,
JoklikW. K.1981 a; Characterization of anti-reovirus immunoglobulins secreted by cloned hybridoma cells lines. Virology 108:134–146
LuftigR. B.,
KilhamS. S.,
HayA. J.,
ZweerinkH. J.,
JoklikW. K.1972; An ultrastructural study of virions and cores of reovirus type 3. Virology 48:170–181
MaizelJ. V.,
PhillipsB. A.,
SummersD. F.1967; Composition of artificially produced and naturally occurring empty capsids of poliovirus type 1. Virology 32:692–699
MustoeT. A.,
RamigR. F.,
SharpeA. H.,
FieldsB. N.1978; Genetics of reovirus: identification of the dsRNA segments encoding the polypeptides of the p and <r size classes. Virology 89:594–604
NorrbyE.,
SkaaretP.1967; The relationship between the soluble antigens and the virion of adenovirus type 3. III. Immunological identification of fiber antigen and isolated vertex capsomer antigen. Virology 32:489497
PetterssonW.,
HÖglunds.1969; Structural properties of adenoviruses. III. Purification and characterization of the adenovirus type 2 penton antigen. Virology 39:90–108
WeinerH. L.,
AultK.,
FieldsB. N.1980; Interaction of reovirus with cell surface receptors. I. Murine and human lymphocytes have a receptor for the hemagglutinin of reovirus type 3. Journal of Immunology 124:2143–2148