1887

Abstract

SUMMARY

We have examined the effects of heat and several chemical inactivating agents on the buoyant density, particle-associated polypeptides and ultrastructure of reovirus particles. Treatment at pH 11 removed polypeptide σl from the outer capsid of reovirus type 2 but not from type 1; resultant particles were unchanged in their buoyant density and morphology. Treatment of reovirus types 2 and 3 with 2.5 M-guanidine-HCl produced particles with unchanged polypeptide content but an increased buoyant density, and caused aggregation of type 3 but not type 2. Treatment with 1 % SDS removed polypeptide σ3 from both types 1 and 2 and increased the buoyant density of the virus particles. The outer capsid of SDS-treated virions was greatly altered and often indistinct. Treatment of type 3 with either 1% phenol or 33% ethanol produced particles that had a full complement of polypeptides, were unaltered in buoyant density, but were greatly aggregated. Thus, these inactivating agents affect reovirus particles in specific and distinct ways. The differential effects of such treatments can thus be used to study the structure and function of the reovirus capsid components.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-63-1-161
1982-11-01
2022-11-28
Loading full text...

Full text loading...

/deliver/fulltext/jgv/63/1/JV0630010161.html?itemId=/content/journal/jgv/10.1099/0022-1317-63-1-161&mimeType=html&fmt=ahah

References

  1. Davis B. D., Dulbecco R., Eisen H. N., Ginsberg H. S. (editors) 1980 Microbiology, 3rd. edn. New York: Harper and Row;
    [Google Scholar]
  2. Drayna D., Fields B. N. 1982; Genetic studies on the mechanism of chemical and physical inactivation of reovirus. Journal of General Virology 63:149–159
    [Google Scholar]
  3. Fields B. N., Raine C., Baum S. G. 1971; Temperature sensitive mutants of reovirus type 3: defects in virus maturation as studied by immunofluorescence and electron microscopy. Virology 43:569–578
    [Google Scholar]
  4. Huismans H., Ajoklik W. K. 1976; Reovirus coded polypeptides in infected cells: isolation of two native monomeric polypeptides with affinity for single-stranded and double-stranded RNA, respectively. Virology 70:411–424
    [Google Scholar]
  5. Joklik W. K. 1972; Studies on the effect of chymotrypsin on reovirions. Virology 49:700–715
    [Google Scholar]
  6. Laemmli U. K. 1970; Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, London 227:680–685
    [Google Scholar]
  7. Lee P. K. W., Hayes E. C., Joklik W. K. 1981 a; Characterization of anti-reovirus immunoglobulins secreted by cloned hybridoma cells lines. Virology 108:134–146
    [Google Scholar]
  8. Lee P. K. W., Hayes E. C., Joklik W. K. 1981 b; Protein σl is the reovirus cell attachment protein. Virology 108:156–163
    [Google Scholar]
  9. Luftig R. B., Kilham S. S., Hay A. J., Zweerink H. J., Joklik W. K. 1972; An ultrastructural study of virions and cores of reovirus type 3. Virology 48:170–181
    [Google Scholar]
  10. Maizel J. V., Phillips B. A., Summers D. F. 1967; Composition of artificially produced and naturally occurring empty capsids of poliovirus type 1. Virology 32:692–699
    [Google Scholar]
  11. Mustoe T. A., Ramig R. F., Sharpe A. H., Fields B. N. 1978; Genetics of reovirus: identification of the dsRNA segments encoding the polypeptides of the p and <r size classes. Virology 89:594–604
    [Google Scholar]
  12. Norrby E., Skaaret P. 1967; The relationship between the soluble antigens and the virion of adenovirus type 3. III. Immunological identification of fiber antigen and isolated vertex capsomer antigen. Virology 32:489497
    [Google Scholar]
  13. Pettersson W., HÖglund s. 1969; Structural properties of adenoviruses. III. Purification and characterization of the adenovirus type 2 penton antigen. Virology 39:90–108
    [Google Scholar]
  14. Silverstein S. C., Astell C., Levin D. H., Schonberg M., Acs G. 1971; The mechanisms of reovirus uncoating and gene activation in vivo. Virology 47:797–806
    [Google Scholar]
  15. Smith R. E., Zweerink H. J., Joklik W. K. 1969; Polypeptide components of virions, top component and cores of reovirus type 3. Virology 39:791–810
    [Google Scholar]
  16. Weiner H. L., Ramig R. F., Mustoe T. A., Fields B. N. 1978; Identification of the gene coding for the hemagglutinin of reovirus. Virology 86:581–584
    [Google Scholar]
  17. Weiner H. L., Ault K., Fields B. N. 1980; Interaction of reovirus with cell surface receptors. I. Murine and human lymphocytes have a receptor for the hemagglutinin of reovirus type 3. Journal of Immunology 124:2143–2148
    [Google Scholar]
  18. Williams R. C., Richards K. E. 1974; Capsid structure of bacteriophage lambda. Journal of Molecular Biology 88:547–550
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-63-1-161
Loading
/content/journal/jgv/10.1099/0022-1317-63-1-161
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error