1887

Abstract

SUMMARY

Enterovirus type 70 (EV70) agglutinated human ‘O’ erythrocytes at 4 °C as well as 22 °C, but visible agglutination was lost when wanned at 37 °C although the virus remained attached to the surface of the erythrocyte. The receptor sites for the virus were neuraminidase-sensitive. A direct involvement of sialic acid on the cell surface in virus-cell interaction was confirmed by the fact that the presence of fetuin or freeacetylneuraminic acid inhibited the haemagglutinating activity of EV70. Similar numbers of virus particles were required for 1 haemagglutinating unit () of EV70 and 1 HAU of mengovirus, whereas 2.6-fold or more of virus particles of echovirus type 7 and type 11 gave the same activity. On the other hand, the number of receptor sites on the cell surface for EV70 was found to be sevenfold more than for mengovirus. Therefore, the erythrocyte receptor for EV70 is different from that for common enteroviruses and similar, though not identical, to the cardiovirus receptor. However, serological tests such as neutralization, complement fixation or haemagglutination inhibition did not reveal any common antigen between EV70 and cardiovirus.

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/content/journal/jgv/10.1099/0022-1317-63-1-141
1982-11-01
2022-01-26
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References

  1. Angel M. A., Burness A. T. H. 1977; The attachment of encephalomyocarditis virustoerythrocytes from several animal species. Virology 83:428–432
    [Google Scholar]
  2. Hierholzer J. C., Suggs M. T. 1969; Standardizedviralhemagglutination and hemagglutination-inhibition tests.I.Standardization of erythrocyte suspensions. Applied Microbiology 18:816–823
    [Google Scholar]
  3. Kono R., Sasagawa A., Ishii K., Sugiura S., Ochi M., Matsumiya H., Uchida Y., Kameyama K., Kaneko M., Sakurai N. 1972; Pandemic of newtypeof conjunctivitis. Lancet i1191–1194
    [Google Scholar]
  4. Kono R., Tajiri E., Miyamura K., Sasagawa A., Tsuruhara T. 1978; Hemagglutination and hemagglutinationinhibition tests withenterovirus type 70. Journal of ClinicalMicrobiology 7:595–598
    [Google Scholar]
  5. Kono R., Sasagawa A., Yamazaki S., Nakazono N., Minami K., Otatsume S., Robin Y., Renaudet J., Cornet M., Afoakwa S. N., Mingle J. A. A., Obinim J. K., Huros A. 1981; Seroepidemiologic studies of acute hemorrhagic conjunctivitisvirus(enterovirus type 70) in West Africa. III. Studies with animalserafrom Ghana and Senegal. American Journal of Epidemiology 114:362–368
    [Google Scholar]
  6. Mak T. W., Callaghan D. J., Colter J. S. 1970; Studies of the pH inactivation of three variants ofmengoencephalomyelitis virus. Virology 40:565–571
    [Google Scholar]
  7. Mirkovic R. R., Kono R., Yin-Murphy M., Sohier R., Schmidt N. J., Melnick J. L. 1973; Enterovirus type 70 : theétiologieagent of pandemic acute haemorrhagic conjunctivitis. Bulletin of theWorld Health Organization 49:341–346
    [Google Scholar]
  8. Miyamura K., Yamazaki S., Tajiri E., Kono R. 1974; Growth characteristics ofacute hemorrhagic conjunctivitis (AHC) virusin monkey kidney cells.I. Effect of temperature on viral growth. Intervirology 4:279–286
    [Google Scholar]
  9. Nbwman J. F. E., Rowlands D. J., Brown F. 1973; A physico-chemical sub-grouping of themammalianpicomaviruses. Journal of General Virology 18:171–180
    [Google Scholar]
  10. Podoplekin V. D. 1964; Effect of tissue cultures onthehemagglutinating properties of echoviruses and mechanism oftheinteraction of these viruses with erythrocytes. Acta virologica 8:254–262
    [Google Scholar]
  11. Podoplekin V. D., Ivanova T. I. 1965; Some intratypic differences between hemagglutinating ECHOviruses. Acta virologica 9:379
    [Google Scholar]
  12. Sasagawa A., Miyamura K., Kono R. 1982; Enterovirus type 70-neutralizing IgM inanimal sera. Japanese Journal of Medical Science and Biology 35:63–73
    [Google Scholar]
  13. Stott E. J., Killington R. A. 1972; Haemagglutination by rhinoviruses. Lancet i1369–1370
    [Google Scholar]
  14. Verlinde J. D., De Baan P. 1949; Sur l’hemagglutination par desvirus poliomyelitiques murins et la destruction enzymatique desrecepteurs de virus poliomyelitique de la cellule receptive. Annalesde l’Institut Pasteur 77:632–641
    [Google Scholar]
  15. Yamazaki S., Natori K., Kono R. 1974; Purification and biophysicalproperties of acute haemorrhagic conjunctivitis virus. Journal ofVirology 14:1357–1360
    [Google Scholar]
  16. Yoshii T., Natori K., Kono R. 1977; Replication of enterovirus 70 in non-primate cellcultures. Journal of General Virology 36:377–384
    [Google Scholar]
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