1887

Abstract

SUMMARY

Twelve temperature-sensitive () and two morphology mutants of nuclear polyhedrosis virus were generated using the mutagen 5-bromo-2′-deoxyuridine (BrdUrd). The mutants grew normally at 25 °C but exhibited abnormal occlusion body formation when grown at 33 °C whereas the morphology mutants produced an abnormal cytopathic effect at 25 °C and 33 °C. None of the mutants was severely restricted for production of non-occluded virus; thus, with the eight mutants and the two morphology mutants incubated at 33 °C the drop in titre was 0.5 to 3.0 log from that at 25 °C. The mutants fell into three groups based on the amount of polyhedrin synthesized at 25 °C and 33 °C in infected cells. The eight mutants were combined with mutants constructed in a previous series of experiments and the collection was sorted into complementation groups. The collection comprised ten groups and the new mutants were sorted into seven of these. It is concluded that gene products of these complementation groups have essential roles in occlusion body formation.

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/content/journal/jgv/10.1099/0022-1317-62-2-369
1982-10-01
2022-06-29
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References

  1. Brown M., Faulkner P. 1975; Factors affecting the yield of virus in a cloned cell line of Trichoplusia ni infected with a nuclear polyhedrosis virus. Journal of Invertebrate Pathology 26:251–257
    [Google Scholar]
  2. Brown M., Faulkner P. 1978; Plaque assay of nuclear polyhedrosis viruses in cell culture. Applied and Environmental Microbiology 36:31–35
    [Google Scholar]
  3. Brown M., Faulkner P. 1980; A partial genetic map of the baculovirus, Autographa califomica nuclear polyhedrosis virus, based on recombination studies with ts mutants. Journal of General Virology 48:247–251
    [Google Scholar]
  4. Brown M., Crawford A. M., Faulkner P. 1979; Genetic analysis of a baculovirus Autographa californica nuclear polyhedrosis virus. I. Isolation of temperature sensitive mutants and assortment into complementation groups. Journal of Virology 31:190–198
    [Google Scholar]
  5. Dobos P., Cochran M. A. 1980; Protein synthesis in cells infected by Autographa californica nuclear polyhedrosis virus (Ac-NPV): the effect of cytosine arabinoside. Virology 103:446–464
    [Google Scholar]
  6. Gardiner G. R., Stockdale H. 1975; Two tissue culture media for production of lepidopteran cells and nuclear polyhedrosis viruses. Journal of Invertebrate Pathology 25:363–370
    [Google Scholar]
  7. Granados R. R. 1976; Infection and replication of insect pathogenic viruses in tissue culture. Advances in Virus Research 20:189–236
    [Google Scholar]
  8. Granados R. R. 1980; Infectivity and mode of action of Baculoviruses. Biotechnology and Bioengineering 22:1377–1405
    [Google Scholar]
  9. Lee H. H., Miller L. K. 1979; Isolation, complementation, and initial characterization of temperature-sensitive mutants of the baculovirus Autographa californica nuclear polyhedrosis virus. Journal of Virology 31:240–252
    [Google Scholar]
  10. Miller L. K. 1981; Construction of a genetic map of the baculovirus Autographa californica nuclear polyhedrosis virus by marker rescue of temperature sensitive mutants. Journal of Virology 39:973–976
    [Google Scholar]
  11. Vaughn J. L., Goodwin R. H., Tompkins G. J., McCawley P. 1977; The establishment of two cell lines from the insect Spodoptera frugiperda (Lepidoptera: Noctuidae). In Vitro 13:213–217
    [Google Scholar]
  12. Volkman L. E., Summers M. D., Hsieh C. H. 1976; Occluded and nonoccluded nuclear polyhedrosis virus grown in Trichoplusia ni: comparative neutralization, comparative infectivity, and in vitro growth studies. Journal of Virology 19:820–832
    [Google Scholar]
  13. Wood H. A., Hughes P. R., Johnston L. B., Langridge W. H. 1981; Increased virulence of Autographa californica nuclear polyhedrosis virus by mutagenesis. Journal of Invertebrate Pathology 38:236–241
    [Google Scholar]
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