1887

Abstract

SUMMARY

Discrete subgenomic DNA fragments were found in three out of thirty-two preparations of adenovirus type 2 incomplete particles grown in human Hep-2 cells and examined over the course of 1 year. One preparation contained three fragments corresponding to 5, 14 and 19% of the genome, another contained a 37% fragment and the third a 40% fragment. Each fragment hybridized exclusively to the left end of the genome. Digestion of the nick-translated 37% fragment with dIII confirmed that it contained the left 37.3% of the genome. Synthesis of these fragments was not dependent on high input multiplicity of infection. Comparable fragments were not found in unpackaged DNA from the corresponding infected cells. This is consistent with the hypothesis that such fragments are generated during virus assembly or, alternatively, may reflect the very small proportion of these fragments relative to the pool of unpackaged DNA within the cells. The possibility that they are generated by errors in DNA replication is discussed.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-62-1-81
1982-09-01
2022-08-11
Loading full text...

Full text loading...

/deliver/fulltext/jgv/62/1/JV0620010081.html?itemId=/content/journal/jgv/10.1099/0022-1317-62-1-81&mimeType=html&fmt=ahah

References

  1. Bodnar J. W., Pearson G. D. 1980; Kinetics of adenovirus DNA replication. II. Initiation of adenovirus DNA replication. Virology 105:357–370
    [Google Scholar]
  2. Brown M., Weber J. 1980; Virion core-like organization of intranuclear adenovirus chromatin late in infection. Virology 107:306–310
    [Google Scholar]
  3. Burlingham B. T., Brown D. T., Doerfler W. 1974; Incomplete particles of adenovirus. I. Characteristics of the DNA associated with incomplete adenovirus of types 2 and 12. Virology 60:419–430
    [Google Scholar]
  4. Challberg M. D., Desiderio S. V., Kelly T. J. Jr 1980; Adenovirus DNA replication in vitro: characterization of a protein covalently linked to nascent DNA strands. Proceedings of the National Academy of Sciences of the United States of America 77:5015–5109
    [Google Scholar]
  5. Daniell E. 1976; Genome structure of incomplete particles of adenovirus. Journal of Virology 19:685–708
    [Google Scholar]
  6. Daniell E. 1981; Subgenomic viral DNA species synthesized in simian cells by human and simian adenoviruses. Journal of Virology 37:620–627
    [Google Scholar]
  7. Daniell E., Mullenbach T. 1978; Synthesis of defective viral DNA in HeLa cells infected with adenovirus type 3. Journal of Virology 26:61–70
    [Google Scholar]
  8. Hammarskjold M. L., Winberg G. 1980; Encapsidation of adenovirus 16 DNA is directed by a small DNA sequence at the left end of the genome. Cell 20:787–795
    [Google Scholar]
  9. Hammarskjold M. L., Winberg G., Norrby E., Wadell G. 1977; Isolation of incomplete adenovirus 16 particles containing viral and host cell DNA. Virology 82:449–461
    [Google Scholar]
  10. HIRT B. 1967; Selective extraction of polyoma DNA from infected mouse cultures. Journal of Molecular Biology 26:365–369
    [Google Scholar]
  11. Khittoo G., Weber J. 1977; Genetic analysis of adenovirus type 2. VI. Adenovirus ts4 gene function is required for DNA encapsidation. Virology 81:126–137
    [Google Scholar]
  12. Khittoo G., Weber J. 1981; The nature of the DNA associated with incomplete particles of adenovirus type 2. Journal of General Virology 54:343–355
    [Google Scholar]
  13. Prage L. S., Hoglund S., Philipson L. 1972; Structural proteins of adenoviruses. III. Characterization of incomplete particles of adenovirus type 3. Virology 49:745–757
    [Google Scholar]
  14. Rajagopalan S., Chinnadurai G. 1979; A unique subgenomic species of adenovirus 2 DNA generated under high multiplicities of infection. Nucleic Acids Research 7:1163–1174
    [Google Scholar]
  15. Tibbetts c. 1977; Viral sequences from incomplete particles of human adenovirus type 7. Cell 12:243–249
    [Google Scholar]
  16. Wahl G. M., Stern M., Stark G. R. 1979; Efficient transfer of large DNA fragments from agarose gels to diazobenzyloxymethyl-paper and rapid hybridization by using dextran sulfate. Proceedings of the National Academy of Sciences of the United States of America 76:3683–3687
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-62-1-81
Loading
/content/journal/jgv/10.1099/0022-1317-62-1-81
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error