@article{mbs:/content/journal/jgv/10.1099/0022-1317-62-1-181, author = "Walker, J. R. and Nagington, J. and Scott, G. M. and Secher, D. S.", title = "An Immunoradiometric Assay of Serum Interferon using a Monoclonal Antibody", journal= "Journal of General Virology", year = "1982", volume = "62", number = "1", pages = "181-185", doi = "https://doi.org/10.1099/0022-1317-62-1-181", url = "https://www.microbiologyresearch.org/content/journal/jgv/10.1099/0022-1317-62-1-181", publisher = "Microbiology Society", issn = "1465-2099", type = "Journal Article", keywords = "monoclonal antibody", keywords = "IFN-α", keywords = "immunoradiometric assay", keywords = "human serum", abstract = "SUMMARY An immunoradiometric assay for human interferon-α (HuIFN-α) has been adapted for the assay of low concentrations of HuIFN-α in human serum. The sensitivity of the assay is 5 to 10 IU/ml and the coefficient of variation less than 10%. The assay was shown to compare well with a biological (antiviral) assay in the measurement of serum interferon following intramuscular injection of HuIFN-α in nine volunteers. Serum interferon was also measured in the serum from 250 normal human donors. Two donors appeared to have detectable levels of HuIFN-α.", }