1887

Abstract

SUMMARY

An immunoradiometric assay for human interferon-α (-α) has been adapted for the assay of low concentrations of -α in human serum. The sensitivity of the assay is 5 to 10 IU/ml and the coefficient of variation less than 10%. The assay was shown to compare well with a biological (antiviral) assay in the measurement of serum interferon following intramuscular injection of -α in nine volunteers. Serum interferon was also measured in the serum from 250 normal human donors. Two donors appeared to have detectable levels of -α.

Loading

Article metrics loading...

/content/journal/jgv/10.1099/0022-1317-62-1-181
1982-09-01
2023-02-02
Loading full text...

Full text loading...

/deliver/fulltext/jgv/62/1/JV0620010181.html?itemId=/content/journal/jgv/10.1099/0022-1317-62-1-181&mimeType=html&fmt=ahah

References

  1. Atherton K. T., Burke D. C. 1975; Interferon induction by viruses and polynucleotides: a differential effect of camptothecin. Journal of General Virology 29:297–304
    [Google Scholar]
  2. Finter N. B., Fantes K. H. 1980 The purity and safety of interferons prepared for clinical use: the case for lymphoblastoid interferon. In Interferon 2 1980 pp. 65–79 Edited by Gresser I. London: Academic Press;
    [Google Scholar]
  3. Goeddel D. U., Leung D. W., Dull T. J., Gross M., Lawn R. M., Mccandliss R., Seeburg P. H., Ullrich A., Yelverton E., Gray P. W. 1981; The structure of eight distinct cloned human leucocyte interferon cDNAs. Nature, London 290:20–25
    [Google Scholar]
  4. Greenwood F. C., Hunter W. M., Glover J. S. 1963; The preparation of 131I-labelled human growth hormone. Biochemical Journal 89:114–123
    [Google Scholar]
  5. Hayes T. G., Yip Y. K., Vilček J. 1979; Le interferon production by human fibroblasts. Virology 98:351–363
    [Google Scholar]
  6. Hooks J. J., Moutsopoulos H. M., Geis S. A., Stahl N. I., Decker J. L., Notkins A. L. 1979; Immune interferon in the circulation of patients with autoimmune disease. New England Journal of Medicine 301: part 1 5–8
    [Google Scholar]
  7. Jensenius J. C., Williams A. F. 1974; The binding of anti-immunoglobulin antibodies to rat thymocytes and thoracic duct lymphocytes. European Journal of Immunology 4:91–97
    [Google Scholar]
  8. Mogensen K. E., Pyhala L., Cantell K. 1975; Raising antibodies to human leucocyte interferon. Acta pathologica et microbiologica scandinavica 83:443–458
    [Google Scholar]
  9. Nagata S., Taira H., Hall A., Johnsrud L., Streuli M., Ecsodi J., Boll W., Cantell K., Weissman C. 1980; Synthesis in E. coli of a polypeptide with human leucocyte interferon activity. Nature, London 284:316–320
    [Google Scholar]
  10. Scott G. M., Secher D. S., Flowers D., Bate J., Cantell K., Tyrrell D. A. J. 1981; Toxicity of interferon. British Medical Journal 282:1345–1348
    [Google Scholar]
  11. Secher D. S. 1981; Immunoradiometric assay of human leukocyte interferon using monoclonal antibody. Nature, London 290:501–503
    [Google Scholar]
  12. Secher D. S., Burke D. C. 1980; A monoclonal antibody for large-scale purification of human leucocyte interferon. Nature, London 285:446–450
    [Google Scholar]
  13. Yelverton E., Leung D., Weck P., Gray P. W., Goeddel D. V. 1981; Bacterial synthesis of a novel human leukocyte interferon. Nucleic Acids Research 9:731–741
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/jgv/10.1099/0022-1317-62-1-181
Loading
/content/journal/jgv/10.1099/0022-1317-62-1-181
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error